%0 Journal Article
%A Claros, Silvia
%A Rodríguez-Losada, Noela
%A Cruz, Encarnación
%A Guerado, Enrique
%A Becerra, José
%A Andrades, José A
%T Characterization of Adult Stem/Progenitor Cell Populations From Bone Marrow in a Three-Dimensional Collagen Gel Culture System.
%D 2012
%@ 0963-6897
%U http://hdl.handle.net/10668/735
%X Stem cell transplantation therapy using mesenchymal stem cells (MSCs) is considered a useful strategy. Although MSCs are commonly isolated by exploiting their plastic adherence, several studies have suggested that there are other populations of stem and/or osteoprogenitor cells which are removed from primary culture during media replacement. Therefore, we developed a three-dimensional (3D) culture system in which adherent and non-adherent stem cells are selected and expanded. Here, we described the characterization of 3D culture-derived cell populations in vitro and the capacity of these cells to differentiate into bone and/or cartilage tissue when placed inside of demineralized bone matrix (DBM) cylinders, implanted subcutaneously into the backs of rat for 2, 4 and 8 weeks. Our results demonstrates that 3D culture cells were a heterogeneous population of uncommitted cells that express pluripotent, hematopoietic, mesenchymal and endothelial specific markers in vitro and can undergo osteogenic differentiation in vivo.
%K Desmineralización Ósea Patológica
%K Molécula de Adhesión Celular del Leucocito Activado
%K Integrina alfa1
%K Integrina alfa2
%K Antígenos CD29
%K Colágeno Tipo I
%K Antígenos CD34
%~