RT Journal Article
T1 A versatile workflow to integrate RNA-seq genomic and transcriptomic data into mechanistic models of signaling pathways.
A1 Garrido-Rodriguez, Martín
A1 Lopez-Lopez, Daniel
A1 Ortuno, Francisco M
A1 Peña-Chilet, María
A1 Muñoz, Eduardo
A1 Calzado, Marco A
A1 Dopazo, Joaquin
AB MIGNON is a workflow for the analysis of RNA-Seq experiments, which not only efficiently manages the estimation of gene expression levels from raw sequencing reads, but also calls genomic variants present in the transcripts analyzed. Moreover, this is the first workflow that provides a framework for the integration of transcriptomic and genomic data based on a mechanistic model of signaling pathway activities that allows a detailed biological interpretation of the results, including a comprehensive functional profiling of cell activity. MIGNON covers the whole process, from reads to signaling circuit activity estimations, using state-of-the-art tools, it is easy to use and it is deployable in different computational environments, allowing an optimized use of the resources available.
YR 2021
FD 2021-02-11
LK http://hdl.handle.net/10668/17147
UL http://hdl.handle.net/10668/17147
LA en
DS RISalud
RD May 11, 2025