RT Journal Article
T1 ADAMTS1 Supports Endothelial Plasticity of Glioblastoma Cells with Relevance for Glioma Progression
A1 Serrano-Garrido, Orlando
A1 Peris-Torres, Carlos
A1 Redondo-García, Silvia
A1 Asenjo, Helena G.
A1 Plaza-Calonge, María del Carmen
A1 Fernandez-Luna, José Luis
A1 Rodríguez-Manzaneque, Juan Carlos
K1 ADAMTS proteases
K1 Endothelial-like phenotype
K1 Extracellular metalloprotease
K1 Glioblastoma
K1 Plasticity
K1 Tumor microenvironment
K1 Cell line
K1 Endothelial cells
K1 Glioma
K1 Proteínas ADAMTS
K1 Fenotipo
K1 Metaloproteasas
K1 Matriz extracelular
K1 Plasticidad de la célula
K1 Microambiente tumoral
K1 Línea celular
K1 Células endoteliales
AB Gliomas in general and the more advanced glioblastomas (GBM) in particular are the most usual tumors of the central nervous system with poor prognosis. GBM patients develop resistance to distinct therapies, in part due to the existence of tumor cell subpopulations with stem-like properties that participate in trans-differentiation events. Within the complex tumor microenvironment, the involvement of extracellular proteases remains poorly understood. The extracellular protease ADAMTS1 has already been reported to contribute to the plasticity of cancer cells. Accordingly, this basic knowledge and the current availability of massive sequencing data from human gliomas, reinforced the development of this work. We first performed an in silico study of ADAMTS1 and endothelial markers in human gliomas, providing the basis to further assess these molecules in several primary glioblastoma-initiating cells and established GBM cells with the ability to acquire an endothelial-like phenotype. Using a co-culture approach of endothelial and GBM cells, we noticed a relevant function of ADAMTS1 in GBM cells leading the organization of endothelial-like networks and, even more significantly, we found a blockade of the formation of tumor-spheres and a deficient response to hypoxia in the absence of ADAMTS1. Our data support a chief role of this protease modulating the phenotypic plasticity of GBM.
PB MDPI
YR 2020
FD 2020-12-31
LK http://hdl.handle.net/10668/4225
UL http://hdl.handle.net/10668/4225
LA en
NO Serrano-Garrido O, Peris-Torres C, Redondo-García S, Asenjo HG, Plaza-Calonge MDC, Fernandez-Luna JL, et al. ADAMTS1 Supports Endothelial Plasticity of Glioblastoma Cells with Relevance for Glioma Progression. Biomolecules. 2020 Dec 31;11(1):44
DS RISalud
RD Apr 8, 2025