RT Journal Article
T1 Early restoration of immune and vascular phenotypes in systemic lupus erythematosus and rheumatoid arthritis patients after B cell depletion.
A1 Perez-Sanchez, Carlos
A1 Cecchi, Irene
A1 Barbarroja, Nuria
A1 Patiño-Trives, Alejandra M
A1 Luque-Tevar, Maria
A1 Perez-Sanchez, Laura
A1 Ibañez-Costa, Alejandro
A1 Arias de la Rosa, Ivan
A1 Ortega, Rafaela
A1 Escudero, Alejandro
A1 Castro, María Carmen
A1 Radin, Massimo
A1 Roccatello, Dario
A1 Sciascia, Savino
A1 Aguirre, Maria Angeles
A1 Collantes, Eduardo
A1 Lopez-Pedrera, Chary
K1 NETosis
K1 endothelial dysfunction
K1 inflammation
K1 rheumatoid arthritis
K1 rituximab
K1 systemic lupus erythematosus
AB This translational multi-centre study explored early changes in serologic variables following B lymphocyte depletion by rituximab (RTX) treatment in systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) patients and investigated in vitro effects on the activity of other immune cells and the vascular endothelium. Eighty-five SLE patients, seventy-five RA patients and ninety healthy donors were enrolled. Two additional cohorts of selected SLE and RA patients were treated with RTX for 3 months. Changes in circulating levels of inflammatory mediators, oxidative stress markers and NETosis-derived bioproducts were evaluated. Serum miRNomes were identified by next-generation sequencing, and RTX-induced changes were delineated. Mechanistic in vitro studies were performed to assess activity profiles. Altered inflammatory, oxidative and NETosis-derived biomolecules were found in SLE and RA patients, closely interconnected and associated to specific miRNA profiles. RTX treatment reduced SLE and RA patients' disease activity, linked to a prominent alteration in those biomolecules and the reversal of altered regulating miRNAs. In vitro studies showed inhibition of NETosis and decline of pro-inflammatory profiles of leucocytes and human umbilical vein endothelial cells (HUVECs) after B cell depletion. This study provides evidence supporting an early RTX-induced re-setting of the pro-inflammatory status in SLE and RA, involving a re-establishment of the homeostatic equilibrium in immune system and the vascular wall.
PB Wiley-Blackwell Publishing Ltd.
YR 2019
FD 2019-06-04
LK http://hdl.handle.net/10668/14310
UL http://hdl.handle.net/10668/14310
LA en
NO érez-Sánchez C, Cecchi I, Barbarroja N, Patiño-Trives AM, Luque-Tévar M, Pérez-Sánchez L, Ibáñez-Costa A, Arias de la Rosa I, Ortega R, Escudero A, Castro MC, Radin M, Roccatello D, Sciascia S, Aguirre MÁ, Collantes E, et al. Early restoration of immune and vascular phenotypes in systemic lupus erythematosus and rheumatoid arthritis patients after B cell depletion. J Cell Mol Med. 2019 Sep;23(9):6308-6318
DS RISalud
RD Apr 5, 2025