RT Journal Article
T1 Genetic diversity of HIV in seminal plasma remains higher than in blood after short-term antiretroviral therapy.
A1 Lopez Zuñiga, Miguel Angel
A1 Chueca, Natalia
A1 de Salazar, Adolfo
A1 Fernandez Caballero, Jose Angel
A1 Gutierrez Valencia, Alicia
A1 Vinuesa Garcia, David
A1 Omar Mohamed Balgahata, Mohamed
A1 Hidalgo Tenorio, Carmen
A1 Lopez-Ruz, Miguel Angel
A1 Garcia, Federico
K1 DNA amplification
K1 HIV
K1 HIV therapeutics
K1 antiretroviral therapy
K1 virology
AB To provide insight on viral kinetics and genetic diversity of HIV in seminal plasma at baseline and 1 month after initiating antiretroviral therapy (ART). Blood and seminal samples from patients with newly diagnosed HIV were obtained before ART initiation (T0) and 1 month after ART initiation (T1). HIV env genetic diversity was studied using deep sequencing Nextera and V3 chemistry in a MiSeq Illumina platform. The number of viral quasispecies (5% cut-off) and Shannon Index were used to analyse diversity. Forty-seven ART-naive patients were recruited between September 2016 and November 2018. At enrolment, the number of quasispecies in blood (median 4 (IQR 2-5)) was lower than in the seminal compartment (median 6, (IQR 4-8)) (p We provide here further evidence of a larger genetic diversity in seminal plasma, both at diagnosis and short term after ART initiation. Our results strengthen previous findings on HIV diversity in seminal plasma. In addition, INIs decrease variability more rapidly than PI and NNRTI in both blood and seminal plasma.
PB BMJ Group
YR 2020
FD 2020-03-17
LK http://hdl.handle.net/10668/15323
UL http://hdl.handle.net/10668/15323
LA en
NO López Zúñiga MÁ, Chueca N, de Salazar A, Fernández Caballero JA, Gutierrez Valencia A, Vinuesa García D, et al. Genetic diversity of HIV in seminal plasma remains higher than in blood after short-term antiretroviral therapy. Sex Transm Infect. 2020 Aug;96(5):337-341
NO This paper has been funded in part by grants from Fondo de InvestigaciónSanitaria (www.isciii.es) (PI18/ 00819), Plan Nacional de I+D+I and Fondo Europeode Desarrollo Regional-FEDER (www.redes/redes/inicio) (RD16/0025/0040).
DS RISalud
RD Apr 18, 2025