RT Journal Article
T1 Infection with multiple hepatitis C virus genotypes detected using commercial tests should be confirmed using next generation sequencing.
A1 Fernandez-Caso, Belen
A1 Fernandez-Caballero, Jose Angel
A1 Chueca, Natalia
A1 Rojo, Eukene
A1 de Salazar, Adolfo
A1 Garcia Buey, Luisa
A1 Cardeñoso, Laura
A1 Garcia, Federico
AB Current HCV genotyping methods may have some limitations in detecting mixed infections. We aimed to determine the accuracy of genotyping and the detection of mixed-genotype infections using the Abbott-RealTime HCV Genotype II assay (Abbott-RT-PCR) in comparison with a Roche-Next Generation Sequencing assay (Roche-NGS). Plasma samples collected from 139 HCV-infected patients tested with Abbott-RT-PCR, 114 with single genotype (GT) and 25 with mixed GTs were genotyped using Roche-NGS. Roche-NGS confirmed all single GTs obtained with Abbott-RT-PCR. One case of Abbott GT 4 was found as GT 1a using Roche-NGS. Genotype 5 was confirmed using Roche-NGS in 75% cases (3 out of 4 cases). Twenty-five patients were identified as having mixed HCVinfections using Abbott-RT-PCR. The concordance between Abbott-RT-PCR and Roche-NGS was 76% (19 out of 25 cases). Three mixed-GT infections identified with the Abbott assay (two (1b + 4); one (1a + 3)) were reported as pure 1b using Roche-NGS. Very divergent results were found for the other three samples. When compared to Roche-NGS, Abbott-RT-PCR has performed excellently for the determination of patients infected with single GTs. For patients that are categorized as having a mixed infection using Abbott-RT-PCR, we recommend an NGS assay as a confirmation test.
PB Nature Publishing Group
YR 2019
FD 2019-04-01
LK http://hdl.handle.net/10668/14175
UL http://hdl.handle.net/10668/14175
LA en
NO Fernández-Caso B, Fernández-Caballero JÁ, Chueca N, Rojo E, de Salazar A, García Buey L, et al. Infection with multiple hepatitis C virus genotypes detected using commercial tests should be confirmed using next generation sequencing. Sci Rep. 2019 Jun 25;9(1):9264.
DS RISalud
RD Apr 11, 2025