RT Journal Article
T1 Evaluation of the speed-oligo direct Mycobacterium tuberculosis assay for molecular detection of mycobacteria in clinical respiratory specimens.
A1 Lara-Oya, Ana
A1 Mendoza-Lopez, Pablo
A1 Rodriguez-Granger, Javier
A1 Fernández-Sánchez, Ana María
A1 Bermúdez-Ruiz, María Pilar
A1 Toro-Peinado, Inmaculada
A1 Palop-Borrás, Begoña
A1 Navarro-Marí, Jose María
A1 Martínez-Lirola, Miguel José
K1 Humanos
K1 Técnicas de diagnóstico molecular
K1 Mycobacterium tuberculosis
K1 Oligonucleótidos
K1 Sensibilidad y especificidad
K1 Esputo
K1 Tuberculosis pulmonar
K1 Técnicas bacteriológicas
AB We present the first evaluation of a novel molecular assay, the Speed-oligo Direct Mycobacterium tuberculosis (SO-DMT) assay, which is based on PCR combined with a dipstick for the detection of mycobacteria and the specific identification of M. tuberculosis complex (MTC) in respiratory specimens. A blind evaluation was carried out in two stages: first, under experimental conditions on convenience samples comprising 20 negative specimens, 44 smear- and culture-positive respiratory specimens, and 11 sputa inoculated with various mycobacterium-related organisms; and second, in the routine workflow of 566 fresh respiratory specimens (4.9% acid-fast bacillus [AFB] smear positives, 7.6% MTC positives, and 1.8% nontuberculous mycobacteria [NTM] culture positives) from two Mycobacterium laboratories. SO-DMT assay showed no reactivity in any of the mycobacterium-free specimens or in those with mycobacterium-related organisms. Compared to culture, the sensitivity in the selected smear-positive specimens was 0.91 (0.92 for MTC and 0.90 for NTM), and there was no molecular detection of NTM in a tuberculosis case or vice versa. With respect to culture and clinical data, the sensitivity, specificity, and positive and negative predictive values for the SO-DMT system in routine specimens were 0.76 (0.93 in smear positives [1.0 for MTC and 0.5 for NTM] and 0.56 in smear negatives [0.68 for MTC and 0.16 for NTM]), 0.99, 0.85 (1.00 in smear positives and 0.68 in smear negatives), and 0.97, respectively. Molecular misidentification of NTM cases occurred when testing 2 gastric aspirates from two children with clinically but not microbiologically confirmed lung tuberculosis. The SO-DMT assay appears to be a fast and easy alternative for detecting mycobacteria and differentiating MTC from NTM in smear-positive respiratory specimens.
PB American Society for Microbiology
SN 0095-1137
YR 2013
FD 2013-01
LK http://hdl.handle.net/10668/2130
UL http://hdl.handle.net/10668/2130
LA en
NO Lara-Oya A, Mendoza-Lopez P, Rodriguez-Granger J, Fernández-Sánchez AM, Bermúdez-Ruiz MP, Toro-Peinado I, et al. Evaluation of the speed-oligo direct Mycobacterium tuberculosis assay for molecular detection of mycobacteria in clinical respiratory specimens. J Clin Microbiol. 2013; 51(1):77-82
NO Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't;
DS RISalud
RD Apr 7, 2025