RT Journal Article
T1 Cytotoxicity and Epidermal Barrier Function Evaluation of Common Antiseptics for Clinical Use in an Artificial Autologous Skin Model
A1 Quiñones-Vico, María I.
A1 Fernández-González, Ana
A1 Pérez-Castejón, Elena
A1 Montero-Vílchez, Trinidad
A1 Arias-Santiago, Salvador
K1 Antiseptic/antibiotic testing
K1 Bioengineered artificial skin substitute
K1 Cell viability
K1 Epidermal barrier function
K1 Regenerative medicine
K1 Wound healing
K1 Antiinfecciosos locales
K1 Piel artificial
K1 Supervivencia celular
K1 Células epidérmicas
K1 Medicina regenerativa
K1 Cicatrización de heridas
AB Bioengineered artificial skin substitutes (BASS) are the main treatment used in addition to autografts when skin injuries involve a large body surface area. Antiseptic/antibiotic treatment is necessary to prevent infections in the BASS implant area. This study aims to evaluate the effect of antiseptics and antibiotics on cell viability, structural integrity, and epidermal barrier function in BASS based on hyaluronic acid during a 28 day follow-up period. Keratinocytes (KTs) and dermal fibroblasts (DFs) were isolated from skin samples and used to establish BASS. The following antibiotic/antiseptic treatment was applied every 48 h: colistin (1%), chlorhexidine digluconate (1%), sodium chloride (0.02%), and polyhexanide (0.1%). Cell viability (LIVE/DEAD® assay), structural integrity (histological evaluation), and epidermal barrier function (trans-epidermal water loss, (TEWL), Tewameter®) were also evaluated. Cell viability percentage of BASS treated with chlorhexidine digluconate was significantly lower (p ≤ 0.001) than the other antiseptics at day 28. Compared to other treatments, chlorhexidine digluconate and polyhexanide significantly affected the epithelium. No significant differences were found regarding epidermal barrier. These results may be useful for treatment protocols after implantation of BASS in patients and evaluating them in clinical practice. BASS represent a suitable model to test in vitro the impact of different treatments of other skin wounds.
PB MDPI
YR 2021
FD 2021-02-08
LK http://hdl.handle.net/10668/4200
UL http://hdl.handle.net/10668/4200
LA en
NO Quiñones-Vico MI, Fernández-González A, Pérez-Castejón E, Montero-Vílchez T, Arias-Santiago S. Cytotoxicity and Epidermal Barrier Function Evaluation of Common Antiseptics for Clinical Use in an Artificial Autologous Skin Model. J Clin Med. 2021 Feb 8;10(4):642
DS RISalud
RD Apr 6, 2025