RT Journal Article
T1 Photographic-Based Optical Evaluation of Tissues and Biomaterials Used for Corneal Surface Repair: A New Easy-Applied Method.
A1 Gonzalez-Andrades, Miguel
A1 Cardona, Juan de la Cruz
A1 Ionescu, Ana Maria
A1 Mosse, Charles A
A1 Brown, Robert A
K1 Animales
K1 Materiales biocompatibles
K1 Colágeno
K1 Córnea
K1 Trasplante de córnea
K1 Diseño de equipo
K1 Luz
K1 Fotografía
K1 Fotometría
K1 Regeneración
K1 Andamios del tejido
K1 Amnios
AB PURPOSETissues and biomaterials used for corneal surface repair require fulfilling specific optical standards prior to implantation in the patient. However, there is not a feasible evaluation method to be applied in clinical or Good Manufacturing Practice settings. In this study, we describe and assess an innovative easy-applied photographic-based method (PBM) for measuring functional optical blurring and transparency in corneal surface grafts.METHODSPlastic compressed collagen scaffolds (PCCS) and multilayered amniotic membranes (AM) samples were optically and histologically evaluated. Transparency and image blurring measures were obtained by PBM, analyzing photographic images of a standardized band pattern taken through the samples. These measures were compared and correlated to those obtained applying the Inverse Adding-Doubling (IAD) technique, which is the gold standard method.RESULTSAll the samples used for optical evaluation by PBM or IAD were histological suitable. PCCS samples presented transmittance values higher than 60%, values that increased with increasing wavelength as determined by IAD. The PBM indicated that PCCS had a transparency ratio (TR) value of 80.3 ± 2.8%, with a blurring index (BI) of 50.6 ± 4.2%. TR and BI obtained from the PBM showed a high correlation (ρ>|0.6|) with the diffuse transmittance and the diffuse reflectance, both determined using the IAD (p<0.005). The AM optical properties showed that there was a largely linear relationship between the blurring and the number of amnion layers, with more layers producing greater blurring.CONCLUSIONSThis innovative proposed method represents an easy-applied technique for evaluating transparency and blurriness of tissues and biomaterials used for corneal surface repair.
PB Public Library of Science
YR 2015
FD 2015-11-13
LK http://hdl.handle.net/10668/2564
UL http://hdl.handle.net/10668/2564
LA en
NO Gonzalez-Andrades M, Cardona Jde LC, Ionescu AM, Mosse CA, Brown RA. Photographic-Based Optical Evaluation of Tissues and Biomaterials Used for Corneal Surface Repair: A New Easy-Applied Method. PLoS ONE. 2015; 10(11):e0142099
NO Journal Article; Correction: doi: 10.1371/journal.pone.0146205.
DS RISalud
RD May 11, 2025