RT Journal Article
T1 Complement Factor C3 Methylation and mRNA Expression Is Associated to BMI and Insulin Resistance in Obesity.
A1 Castellano-Castillo, Daniel
A1 Moreno-Indias, Isabel
A1 Fernandez-Garcia, Jose Carlos
A1 Clemente-Postigo, Mercedes
A1 Castro-Cabezas, Manuel
A1 Tinahones, Francisco Jose
A1 Queipo-Ortuño, Maria Isabel
A1 Cardona, Fernando
K1 ASP
K1 C3
K1 DNA methylation
K1 complement factor
K1 insulin resistance
K1 obesity
AB  Epigenetic marks, and especially DNA methylation, are becoming an important factor in obesity, which could help to explain its etiology and associated comorbidities. Adipose tissue, now considered as an important endocrine organ, produces complement system factors. Complementcomponent 3 (C3) turns out to be an important protein in metabolic disorders, via either inflammation or the C3 subproduct acylation stimulating protein (ASP) which directly stimulates lipid storage. In this study, we analyze C3 DNA methylation in adipose tissue from subjects with a different gradeof obesity. Adipose tissue samples were collected from subjects with a different degree of obesity determined by their body mass index (BMI) as: Overweight subjects (BMI ≥ 25 and <30), obese class 1/2 subjects (BMI ≥ 30 and <40) and obese class 3 subjects (BMI ≥ 40). C3 DNA methylationwas measured for 7 CpGs by pyrosequencition using the Pyromark technology (Qiagen, Madrid Spain). C3 messenger RNA (mRNA) levels were analyzed by pre-designed Taqman assays (Applied biosystems, Foster City, CA, USA) and ASP/C3a was measured using a ELISA kit. The data wereanalyzed using the statistic package SPSS. C3 DNA methylation levels were lower in the morbid obese group. Accordingly, C3 methylation correlated negatively with BMI and leptin. However, C3 mRNA levels were more associated with insulin resistance, and positive correlations with insulin,glucose and homeostasis model assessment-estimated insulin resistance (HOMA-IR) existed. ASP correlated negatively with high density lipoprotein (HDL) cholesterol. C3 methylation levels were associated to adiposity variables, such as BMI and leptin, while the C3 mRNA levels were associatedto glucose metabolism.
PB MDPI AG
SN 2073-4425
YR 2018
FD 2018-08-08
LK http://hdl.handle.net/10668/12832
UL http://hdl.handle.net/10668/12832
LA en
NO Castellano-Castillo D, Moreno-Indias I, Fernandez-Garcia JC, Clemente-Postigo M, Castro-Cabezas M, Tinahones FJ, et al. Complement factor C3 methylation and mRNA expression is associated to BMI and insulin resistance in obesity. Genes (Basel). 2018 Aug 13;9(8):410
DS RISalud
RD Apr 18, 2025