RT Journal Article
T1 Selection of Tumor-Specific Cytotoxic T Lymphocytes in Acute Myeloid Leukemia Patients Through the Identification of T-Cells Capable to Establish Stable Interactions With the Leukemic Cells: "Doublet Technology".
A1 Garcia-Guerrero, Estefania
A1 Sanchez-Abarca, Luís I
A1 Domingo, Esther
A1 Ramos, Teresa L
A1 Bejarano-Garcia, Jose A
A1 Gonzalez-Campos, Jose A
A1 Caballero-Velazquez, Teresa
A1 Perez-Simon, Jose A
K1 T cell-tumor cell synapse
K1 acute myeloid leukemia
K1 cell selection
K1 immunotherapy
K1 tumor-specific T cells
AB The relevance of the immune system in cancer has long been studied. Autologous adoptive T cell therapies, based on the use of tumor infiltrating lymphocytes (TILs), have made great progress in recent years for the treatment of solid tumors, especially melanoma. However, further work is needed to isolate tumor-reactive T cells among patients diagnosed with hematologic malignancies. The dynamics of the interaction between T cells and antigen presenting cells (APC) dictate the quality of the immune responses. While stable joints between target cells and T lymphocytes lead to the induction of T cell activation and immune response, brief contacts contribute to the induction of immune-tolerance. Taking advantage of the strong interaction between target cell and activated T-cells, we show the feasibility to identify and isolate tumor-specific cytotoxic T lymphocytes (CTLs) from acute myeloid leukemia (AML) patients by flow cytometry. Using this technology, CTLs bound through T cell receptor (TCR) to tumor cells can be identified in peripheral blood and bone marrow and subsequently selected and isolated by FACS-based cell sorting. These CTLs display higher percentage of effector cells and marked cytotoxic activity against AML blasts. In conclusion, we have developed a new procedure to identify and select specific cytotoxic T cells in patients diagnosed with acute myeloid leukemia.
PB Frontiers Research Foundation
YR 2018
FD 2018-09-03
LK http://hdl.handle.net/10668/12967
UL http://hdl.handle.net/10668/12967
LA en
NO García-Guerrero E, Sánchez-Abarca LI, Domingo E, Ramos TL, Bejarano-García JA, Gonzalez-Campos JA, et al. Selection of Tumor-Specific Cytotoxic T Lymphocytes in Acute Myeloid Leukemia Patients Through the Identification of T-Cells Capable to Establish Stable Interactions With the Leukemic Cells: "Doublet Technology". Front Immunol. 2018 Sep 3;9:1971.
DS RISalud
RD Apr 4, 2025