RT Journal Article
T1 Functional Changes of T-Cell Subsets with Age and CMV Infection.
A1 Hassouneh, Fakhri
A1 Goldeck, David
A1 Pera, Alejandra
A1 van Heemst, Diana
A1 Slagboom, P. Eline
A1 Pawelec, Graham
A1 Solana, Rafael
K1 CD57
K1 T-cell response
K1 Aging
K1 Cytomegalovirus
AB Cytomegalovirus (CMV) latent infection and aging contribute to alterations in the function and phenotype of the T-cell pool. We have demonstrated that CMV-seropositivity is associated with the expansion of polyfunctional CD57+ T-cells in young and middle-aged individuals in response to different stimuli. Here, we expand our results on the effects of age and CMV infection on T-cell functionality in a cohort of healthy middle-aged and older individuals stratified by CMV serostatus. Specifically, we studied the polyfunctional responses (degranulation, IFN-γ and TNF-α production) of CD4+, CD8+, CD8+CD56+ (NKT-like), and CD4-CD8- (DN) T-cells according to CD57 expression in response to Staphylococcal Enterotoxin B (SEB). Our results show that CD57 expression by T-cells is not only a hallmark of CMV infection in young individuals but also at older ages. CD57+ T-cells are more polyfunctional than CD57- T-cells regardless of age. CMV-seronegative individuals have no or a very low percentages of cytotoxic CD4+ T-cells (CD1017a+) and CD4+CD57+ T-cells, supporting the notion that the expansion of these T-cells only occurs in the context of CMV infection. There was a functional shift in T-cells associated with CMV seropositivity, except in the NKT-like subset. Here, we show that the effect of CMV infection and age differ among T-cell subsets and that CMV is the major driving force for the expansion of highly polyfunctional CD57+ T-cells, emphasizing the necessity of considering CMV serology in any study of immunosenescence.
PB MDPI
YR 2021
FD 2021-09-09
LK http://hdl.handle.net/10668/18580
UL http://hdl.handle.net/10668/18580
LA en
NO Hassouneh F, Goldeck D, Pera A, van Heemst D, Slagboom PE, Pawelec G, et al.  Functional Changes of T-Cell Subsets with Age and CMV Infection. Int J Mol Sci. 2021 Sep 15;22(18):9973
DS RISalud
RD Apr 11, 2025