RT Journal Article
T1 Exploring the Healthy Eye Microbiota Niche in a Multicenter Study.
A1 Borroni, Davide
A1 Paytuvi-Gallart, Andreu
A1 Sanseverino, Walter
A1 Gomez-Huertas, Carmen
A1 Bonci, Paola
A1 Romano, Vito
A1 Giannaccare, Giuseppe
A1 Rechichi, Miguel
A1 Meduri, Alessandro
A1 Oliverio, Giovanni William
A1 Rocha-de-Lossada, Carlos
K1 16
K1 OSDI
K1 Eye
K1 Healthy
K1 Metagenomics
K1 Microbiome
AB This study aims to explore and characterize healthy eye microbiota. Healthy subjects older than 18 years were selected for this descriptive cross-sectional study. Samples were collected with an eSwab with 1 mL of Liquid Amies Medium (Copan Brescia, Italy). Following DNA extraction, libraries preparation, and amplification, PCR products were purified and end-repaired for barcode ligation. Libraries were pooled to a final concentration of 26 pM. Template preparation was performed with Ion Chef according to Ion 510, Ion 520, and Ion 530 Kit-Chef protocol. Sequencing of the amplicon libraries was carried out on a 520 or 530 chip using the Ion Torrent S5 system (Thermo Fisher; Waltham, MA, USA). Raw reads were analyzed with GAIA (v 2.02). Healthy eye microbiota is a low-diversity microbiome. The vast majority of the 137 analyzed samples were highly enriched with Staphylococcus, whereas only in a few of them, other genera such as Bacillus, Pseudomonas, and Corynebacterium predominate. We found an average of 88 genera with an average Shannon index of 0.65. We identified nine different ECSTs. A better understanding of healthy eye microbiota has the potential to improve disease diagnosis and personalized regimens to promote health.
PB MDPI
YR 2022
FD 2022-09-06
LK http://hdl.handle.net/10668/21194
UL http://hdl.handle.net/10668/21194
LA en
NO Borroni D, Paytuví-Gallart A, Sanseverino W, Gómez-Huertas C, Bonci P, Romano V, et al. Exploring the Healthy Eye Microbiota Niche in a Multicenter Study. Int J Mol Sci. 2022 Sep 6;23(18):10229
DS RISalud
RD Apr 7, 2025