RT Generic
T1 Dynamic Reorganization of the Cytoskeleton during Apoptosis: The Two Coffins Hypothesis
A1 Povea-Cabello, Suleva
A1 Oropesa-Avila, Manuel
A1 de la Cruz-Ojeda, Patricia
A1 Villanueva-Paz, Marina
A1 de la Mata, Mario
A1 Miguel Suarez-Rivero, Juan
A1 Alvarez-Cordoba, Monica
A1 Villalon-Garcia, Irene
A1 Cotan, David
A1 Ybot-Gonzalez, Patricia
A1 Sanchez-Alcazar, Jose A.
K1 apoptosis
K1 apoptotic microtubule network
K1 microtubules
K1 actin filaments
K1 genotoxic drugs
K1 Programmed cell-death
K1 Intermediate-filaments
K1 Microtubule dynamics
K1 Secondary necrosis
K1 Execution phase
K1 Genetic-control
K1 Cycle entry
K1 Activation
K1 Protein
K1 Pathways
AB During apoptosis, cells undergo characteristic morphological changes in which the cytoskeleton plays an active role. The cytoskeleton rearrangements have been mainly attributed to actinomyosin ring contraction, while microtubule and intermediate filaments are depolymerized at early stages of apoptosis. However, recent results have shown that microtubules are reorganized during the execution phase of apoptosis forming an apoptotic microtubule network (AMN). Evidence suggests that AMN is required to maintain plasma membrane integrity and cell morphology during the execution phase of apoptosis. The new two coffins hypothesis proposes that both AMN and apoptotic cells can adopt two morphological patterns, round or irregular, which result from different cytoskeleton kinetic reorganization during the execution phase of apoptosis induced by genotoxic agents. In addition, round and irregular-shaped apoptosis showed different biological properties with respect to AMN maintenance, plasma membrane integrity and phagocyte responses. These findings suggest that knowing the type of apoptosis may be important to predict how fast apoptotic cells undergo secondary necrosis and the subsequent immune response. From a pathological point of view, round-shaped apoptosis can be seen as a physiological and controlled type of apoptosis, while irregular-shaped apoptosis can be considered as a pathological type of cell death closer to necrosis.
PB Mdpi
SN 1422-0067
YR 2017
FD 2017-11-01
LK http://hdl.handle.net/10668/19286
UL http://hdl.handle.net/10668/19286
LA en
NO Povea-Cabello S, Oropesa-Ávila M, de la Cruz-Ojeda P, Villanueva-Paz M, de la Mata M, Suárez-Rivero JM, et al. Dynamic Reorganization of the Cytoskeleton during Apoptosis: The Two Coffins Hypothesis. Int J Mol Sci. 2017 Nov 11;18(11)
NO This work was supported by PI16/00786 grant, Instituto de Salud Carlos III, Spain and Fondo Europeo de Desarrollo Regional (FEDER-Unión Europea), and by AEPMI (Asociación de Enfermos de Patología Mitocondrial) and ENACH (Asociación de Enfermos de Neurodegeneración con Acumulación Cerebral de Hierro).
DS RISalud
RD Apr 6, 2025