Direct Detection of miR-122 in Hepatotoxicity Using Dynamic Chemical Labeling Overcomes Stability and isomiR Challenges.

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dc.contributor.authorLópez-Longarela, Barbara
dc.contributor.authorMorrison, Emma E
dc.contributor.authorTranter, John D
dc.contributor.authorChahman-Vos, Lianne
dc.contributor.authorLéonard, Jean-François
dc.contributor.authorGautier, Jean-Charles
dc.contributor.authorLaurent, Sébastien
dc.contributor.authorLartigau, Aude
dc.contributor.authorBoitier, Eric
dc.contributor.authorSautier, Lucile
dc.contributor.authorCarmona-Saez, Pedro
dc.contributor.authorMartorell-Marugan, Jordi
dc.contributor.authorMellanby, Richard J
dc.contributor.authorPernagallo, Salvatore
dc.contributor.authorIlyine, Hugh
dc.contributor.authorRissin, David M
dc.contributor.authorDuffy, David C
dc.contributor.authorDear, James W
dc.contributor.authorDíaz-Mochón, Juan J
dc.date.accessioned2025-01-07T17:29:53Z
dc.date.available2025-01-07T17:29:53Z
dc.date.issued2020-01-27
dc.description.abstractCirculating microRNAs are biomarkers reported to be stable and translational across species. MicroRNA-122 (miR-122) is a hepatocyte-specific microRNA biomarker for drug-induced liver injury (DILI). We developed a single molecule, dynamic chemical labeling (DCL) assay to directly detect miR-122 in blood. The DCL assay specifically measured miR-122 directly from 10 μL of serum or plasma without any extraction steps, with a limit of detection of 1.32 pM that enabled the identification of DILI. Testing of 192 human serum samples showed that DCL accurately identified patients at risk of DILI after acetaminophen overdose (area under ROC curve 0.98 (95% CI; 0.96-1), P
dc.identifier.doi10.1021/acs.analchem.9b05449
dc.identifier.essn1520-6882
dc.identifier.pmid31939284
dc.identifier.unpaywallURLhttp://hdl.handle.net/20.500.11820/6a3d708a-fc7c-4d13-939a-e1fedda53e81
dc.identifier.urihttps://hdl.handle.net/10668/28411
dc.issue.number4
dc.journal.titleAnalytical chemistry
dc.journal.titleabbreviationAnal Chem
dc.language.isoen
dc.organizationCentro Pfizer-Andalucía de Genómica e Investigación Oncológica (GENYO)
dc.organizationCentro Pfizer-Andalucía de Genómica e Investigación Oncológica (GENYO)
dc.page.number3388-3395
dc.pubmedtypeJournal Article
dc.pubmedtypeResearch Support, Non-U.S. Gov't
dc.rights.accessRightsopen access
dc.subject.meshAcetaminophen
dc.subject.meshAdolescent
dc.subject.meshAdult
dc.subject.meshAnimals
dc.subject.meshBiomarkers
dc.subject.meshChemical and Drug Induced Liver Injury
dc.subject.meshDogs
dc.subject.meshHepatocytes
dc.subject.meshHumans
dc.subject.meshMale
dc.subject.meshMicroRNAs
dc.subject.meshRats
dc.subject.meshYoung Adult
dc.titleDirect Detection of miR-122 in Hepatotoxicity Using Dynamic Chemical Labeling Overcomes Stability and isomiR Challenges.
dc.typeresearch article
dc.type.hasVersionSMUR
dc.volume.number92

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