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dc.contributor.authorTinahones Madueño, Francisco José-
dc.contributor.authorSalas, Julian-
dc.contributor.authorMayas Torres, María Dolores-
dc.contributor.authorRuiz-Villalba, Adrián-
dc.contributor.authorMacías-González, Manuel-
dc.contributor.authorGarrido-Sánchez, Lourdes-
dc.contributor.authorDeMora, Manuel-
dc.contributor.authorMoreno-Santos, Inmaculada-
dc.contributor.authorBernal, Rosa-
dc.contributor.authorCardona Díaz, Fernando-
dc.contributor.authorEl Bekay, Rajaa-
dc.identifier.citationTinahones F, Salas J, Mayas MD, Ruiz-Villalba A, Macias-Gonzalez M, Garrido-Sanchez L, et al. VEGF gene expression in adult human thymus fat: a correlative study with hypoxic induced factor and cyclooxygenase-2. PLoS ONE; 4(12):e8213es
dc.identifier.issn1932-6203 (Online)-
dc.descriptionJournal Article; Research Support, Non-U.S. Gov't;es
dc.description.abstractIt is well known that the adult human thymus degenerates into fat tissue; however, it has never been considered as a potential source of angiogenic factors. Recently, we have described that this fat (TAT) produces angiogenic factors and induces human endothelial cell proliferation and migration, indicating its potential angiogenic properties. DESIGN Adult thymus fat and subcutaneous adipose tissue specimens were obtained from 28 patients undergoing cardiac surgery, making this tissue readily available as a prime source of adipose tissue. We focused our investigation on determining VEGF gene expression and characterizing the different genes, mediators of inflammation and adipogenesis, and which are known to play a relevant role in angiogenesis regulation. RESULTS We found that VEGF-A was the isoform most expressed in TAT. This expression was accompanied by an upregulation of HIF-1alpha, COX-2 and HO-1 proteins, and by increased HIF-1 DNA binding activity, compared to SAT. Furthermore, we observed that TAT contains a high percentage of mature adipocytes, 0.25% of macrophage cells, 15% of endothelial cells and a very low percentage of thymocyte cells, suggesting the cellular variability of TAT, which could explain the differences in gene expression observed in TAT. Subsequently, we showed that the expression of genes known as adipogenic mediators, including PPARgamma1/gamma2, FABP-4 and adiponectin was similar in both TAT and SAT. Moreover the expression of these latter genes presented a significantly positive correlation with VEGF, suggesting the potential association between VEGF and the generation of adipose tissue in adult thymus. CONCLUSION Here we suggest that this fat has a potential angiogenic function related to ongoing adipogenesis, which substitutes immune functions within the adult thymus. The expression of VEGF seems to be associated with COX-2, HO-1 and adipogenesis related genes, suggesting the importance that this new fat has acquired in research in relation to adipogenesis and
dc.description.sponsorshipThis work was supported in part by grants from Andalusian Health Service [SAS PI-0251]; R. El Bekay, M. Macias and F. Cardona are recipients of a post doctoral grant ‘Miguel Servet’(FIS-2007) [CP07/00288, CP04/00039, CP07/0095] from the Spanish Ministry of Health; and part of this project was supported by grant from Spanish Ministry of Health (FIS) [PI070953].es
dc.publisherPublic Library of Sciencees
dc.relation.ispartofPloS onees
dc.subjectFABP4 protein, humanes
dc.subjectPPAR gammaes
dc.subjectProtein Isoformses
dc.subjectVEGFA protein, humanes
dc.subjectCyclooxygenase 2es
dc.subjectPTGS2 protein, humanes
dc.subjectPPAR gammaes
dc.subjectIsoformas de Proteínases
dc.subjectCiclooxigenasa 2es
dc.subjectTejido adiposoes
dc.subjectMarcadores Biológicoses
dc.subjectMovimiento Celulares
dc.subjectCiclooxigenasa 2es
dc.subjectProteínas de Unión a Ácidos Grasoses
dc.subjectRegulación de la Expresión Génicaes
dc.subjectHemo-Oxigenasa 1es
dc.subjectHipoxia Induciblees
dc.subjectUnión proteicaes
dc.subjectIsoformas de proteínases
dc.subjectARN Mensajeroes
dc.subjectTejido subcutáneoes
dc.subjectFactor A de crecimiento endotelial vasculares
dc.subject.meshMedical Subject Headings::Anatomy::Tissues::Connective Tissue::Adipose Tissuees
dc.subject.meshMedical Subject Headings::Named Groups::Persons::Age Groups::Adultes
dc.subject.meshMedical Subject Headings::Named Groups::Persons::Age Groups::Adult::Agedes
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Anti-Inflammatory Agentses
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Biological Factors::Biological Markerses
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Movementes
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Multienzyme Complexes::Prostaglandin-Endoperoxide Synthases::Cyclooxygenase 2es
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Structures::Nucleic Acid Conformationes
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Carrier Proteins::Fatty Acid-Binding Proteinses
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression Regulationes
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Oxidoreductases::Oxygenases::Mixed Function Oxygenases::Heme Oxygenase (Decyclizing)es
dc.subject.meshMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humanses
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Transcription Factors::Basic Helix-Loop-Helix Transcription Factors::Hypoxia-Inducible Factor 1es
dc.subject.meshMedical Subject Headings::Diseases::Pathological Conditions, Signs and Symptoms::Pathologic Processes::Inflammationes
dc.subject.meshMedical Subject Headings::Anatomy::Cells::Connective Tissue Cells::Macrophageses
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::Protein Bindinges
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Protein Isoformses
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::RNA::RNA, Messengeres
dc.subject.meshMedical Subject Headings::Anatomy::Tissues::Connective Tissue::Subcutaneous Tissuees
dc.subject.meshMedical Subject Headings::Anatomy::Tissues::Lymphoid Tissue::Thymus Glandes
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides::Intercellular Signaling Peptides and Proteins::Angiogenic Proteins::Vascular Endothelial Growth Factors::Vascular Endothelial Growth Factor Aes
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Hormones, Hormone Substitutes, and Hormone Antagonists::Hormones::Peptide Hormones::Adipokines::Adiponectines
dc.titleVEGF gene expression in adult human thymus fat: a correlative study with hypoxic induced factor and
dc.rights.accessRightsAcceso abiertoes
dc.contributor.authoraffiliation[Tinahones Madueño,FJ; Mayas Torres,MD; Macías-González,M; Garrido-Sánchez,L; Moreno-Santos,I; Bernal,R; Cardona Díaz,F; El Bekay,R] Instituto Carlos III, Madrid, Spain. [Tinahones Madueño,FJ] Servicio de Endocrinología, Hospital Universitario Virgen de la Victoria, Málaga, Spain. [Salas,J] Departamento de Cirugía Cardiovascular, Hospital Carlos Haya, Málaga, Spain. [Ruiz-Villalba,A; El Bekay,R] Fundación Instituto Mediterráneo para el Avance de la Biotecnología y la Investigación Sanitaria, Laboratorio de Investigación Biomédica, Hospital Universitario Virgen de la Victoria, Málaga, Spain. [DeMora,M] Departamento de Enfermedades Cardiovasculares, Hospital Carlos Haya, Málaga,
Appears in Collections:01- Artículos - Hospital Regional de Málaga
01- Artículos - Hospital Virgen de la Victoria
01- Artículos - IBIMA. Instituto de Investigación Biomédica de Málaga

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