Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/683
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dc.contributor.authorSanchez-Quevedo, M.C.-
dc.contributor.authorAlaminos, M.-
dc.contributor.authorCapitan, L.M.-
dc.contributor.authorMoreu, G.-
dc.contributor.authorGarzon, I.-
dc.contributor.authorCrespo, P.V.-
dc.contributor.authorCampos, A-
dc.date.accessioned2012-12-03T10:06:09Z-
dc.date.available2012-12-03T10:06:09Z-
dc.date.issued2007-06-
dc.identifier.citationSanchez-Quevedo MC, Alaminos M, Capitan LM, Moreu G, Garzon I et al.Histological and histochemical evaluation of human oral mucosa constructs developed by tissue engineering. Histol Histopathol. 2007 Jun;22(6):631-40es
dc.identifier.issn0213-3911 (Print)-
dc.identifier.issn1699-5848 (Online)-
dc.identifier.urihttp://hdl.handle.net/10668/683-
dc.descriptionJournal Article; Research Support, Non-U.S. Gov't;es
dc.description.abstractReconstruction of large oral mucosa defects is often challenging, since the shortage of healthy oral mucosa to replace the excised tissues is very common. In this context, tissue engineering techniques may provide a source of autologous tissues available for transplant in these patients. In this work, we developed a new model of artificial oral mucosa generated by tissue engineering using a fibrin-agarose scaffold. For that purpose, we generated primary cultures of human oral mucosa fibroblasts and keratinocytes from small biopsies of normal oral mucosa using enzymatic treatments. Then we determined the viability of the cultured cells by electron probe quantitative X-ray microanalysis, and we demonstrated that most of the cells in the primary cultures were alive and had high K/Na ratios. Once cell viability was determined, we used the cultured fibroblasts and keratinocytes to develop an artificial oral mucosa construct by using a fibrin-agarose extracellular matrix and a sequential culture technique using porous culture inserts. Histological analysis of the artificial tissues showed high similarities with normal oral mucosa controls. The epithelium of the oral substitutes had several layers, with desmosomes and apical microvilli and microplicae. Both the controls and the oral mucosa substitutes showed high suprabasal expression of cytokeratin 13 and low expression of cytokeratin 10. All these results suggest that our model of oral mucosa using fibrin-agarose scaffolds show several similarities with native human oral mucosa.es
dc.description.sponsorshipThis work was supported by the grants FIS 03/0141 and FIS 04/1306 from the Spanish National Ministry of Health (Instituto de Salud Carlos III) and by CM 2005/011 from Junta de Andalucia.es
dc.language.isoenes
dc.publisherUniversidad de Murciaes
dc.relation.ispartofHistology and histopathologyes
dc.subjectTécnicas de Cultivo de Célulaes
dc.subjectSupervivencia Celulares
dc.subjectCélulas Cultivadases
dc.subjectMicroanálisis por Sonda Electrónicaes
dc.subjectFibrinaes
dc.subjectFibroblastoses
dc.subjectExpresión Génicaes
dc.subjectPerfilación de la Expresión Génicaes
dc.subjectHumanoses
dc.subjectHidrogeleses
dc.subjectInmunohistoquímicaes
dc.subjectQueratinocitoses
dc.subjectMicroscopía Electrónicaes
dc.subjectMucosa Bucales
dc.subjectAnálisis de Secuencia en Orden de Oligonucleótidoes
dc.subjectSefarosaes
dc.subjectIngeniería de Tejidoses
dc.subjectFibrin-agarosees
dc.subjectConstructses
dc.subjectTissue engineeringes
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Survivales
dc.subject.meshMedical Subject Headings::Anatomy::Cells::Cells, Culturedes
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Imaging::Microscopy::Microscopy, Electron::Electron Probe Microanalysises
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Fibrines
dc.subject.meshMedical Subject Headings::Anatomy::Cells::Connective Tissue Cells::Fibroblastses
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expressiones
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Gene Expression Profilinges
dc.subject.meshMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humanses
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Complex Mixtures::Colloids::Gels::Hydrogelses
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Cytological Techniques::Histocytochemistry::Immunohistochemistryes
dc.subject.meshMedical Subject Headings::Anatomy::Cells::Epithelial Cells::Keratinocyteses
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Imaging::Microscopy::Microscopy, Electrones
dc.subject.meshMedical Subject Headings::Anatomy::Tissues::Membranes::Mucous Membrane::Mouth Mucosaes
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Microchip Analytical Procedures::Microarray Analysis::Oligonucleotide Array Sequence Analysises
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Carbohydrates::Polysaccharides::Sepharosees
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Culture Techniques::Cell Engineering::Tissue Engineeringes
dc.titleHistological and histochemical evaluation of human oral mucosa constructs developed by tissue engineeringes
dc.typeinfo:eu-repo/semantics/articlees
dc.description.versionYeses
dc.identifier.pmid17357093-
dc.rights.accessRightsAcceso abiertoes
dc.type.versioninfo:eu-repo/semantics/publishedes
dc.relation.publisherversionhttp://www.hh.um.es/Abstracts/Vol_22/22_6/22_6_631.htmes
dc.contributor.authoraffiliation[Sanchez-Quevedo,MC; Alaminos,M; Garzon,I; Crespo,PV; Campos,A.] Department of Histology, University of Granada, Spain. [Alaminos,M] Fundación Hospital Clínico, Granada, Spain.[Capitan,LM] Division of Oral and Maxillofacial Surgery, University Hospital Virgen de las Nieves, Granada, Spain. [Moreu,G] Department of Stomatology, University of Granada, Spain.es
dc.type.subtypeArtículoes
Appears in Collections:01- Artículos - Hospital San Cecilio
01- Artículos - Hospital Virgen de las Nieves

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