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Título : Epidermal growth factor receptor and epididymis invasion as prognostic biomarkers in clinical stage I testicular germ cell tumours
Autor : Sanmamed, Miguel F
Esteban, E
Uriol, E
Zarate, R
Capelan, M
Muriel, C
Crespo, G
Berros, J P
Pardo-Coto, P
Perez, Q
Alvarez-Fernández, C
Jiménez Fonseca, P
Luque, M
Astudillo, A
Filiación: [Sanmamed,MF] Department of Immunobiology, School of Medicine, Yale University, New Haven, USA. [Sanmamed,MF; Estebam.E: Iropñ.E: Berrps.K^: Perez,Q; Alvarez-Fernández,C; Jiménez Fonseca,P; Luque,M] Department of Medical Oncology, Hospital Universitario Central de Asturias, Oviedo, Spain. [Zarate,R] Clinical Genetics Unit, Clínica Universidad de Navarra, Pamplona, Spain. [Capelan,M] Breast Unit, Royal Marsden NHS Foundation Trust, London, UK. [Muriel,C] Department of Medical Oncology, Hospital Regional Universitario Carlos Haya, Málaga, Spain. [Crespo,G] Department of Medical Oncology, Hospital Universitario de Burgos, Burgos, Spain. [Pardo-Coto,P] Department of Medical Oncology, Centro Médico de Asturias, Oviedo, Spain. [Astudillo,A] Department of Pathology, Hospital Universitario Central de Asturias, Oviedo, Spain.
Palabras clave : Testicular germ cell tumour;EGFR;Epididymis invarsion;hMSH-2;MSI;Carcinoma embrionario;Epidídimo;Exones;Humanos;Inmunohistoquímica;Masculino;Metilación;Inestabilidad de microsatélites;Recurrencia local de neoplasia;Orquiectomía;Pronóstico;Recurrence;Red Testicular;Neoplasias Testiculares
MeSH: Medical Subject Headings::Diseases::Neoplasms::Neoplasms by Histologic Type::Neoplasms, Germ Cell and Embryonal::Carcinoma, Embryonal
Medical Subject Headings::Anatomy::Urogenital System::Genitalia::Genitalia, Male::Epididymis
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Structures::Genome::Genome Components::Genes::Gene Components::Exons
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Histological Techniques::Histocytochemistry::Immunohistochemistry
Medical Subject Headings::Check Tags::Male
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Chemical Processes::Biochemical Processes::Alkylation::Methylation
Medical Subject Headings::Diseases::Pathological Conditions, Signs and Symptoms::Pathologic Processes::Genomic Instability::Microsatellite Instability
Medical Subject Headings::Diseases::Neoplasms::Neoplastic Processes::Neoplasm Recurrence, Local
Medical Subject Headings::Diseases::Neoplasms::Neoplasms by Histologic Type::Neoplasms, Germ Cell and Embryonal
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Surgical Procedures, Operative::Urogenital Surgical Procedures::Urologic Surgical Procedures::Urologic Surgical Procedures, Male::Orchiectomy
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Prognosis
Medical Subject Headings::Diseases::Pathological Conditions, Signs and Symptoms::Pathologic Processes::Disease Attributes::Recurrence
Medical Subject Headings::Anatomy::Urogenital System::Genitalia::Genitalia, Male::Testis::Rete Testis
Medical Subject Headings::Diseases::Male Urogenital Diseases::Urogenital Neoplasms::Genital Neoplasms, Male::Testicular Neoplasms
Fecha de publicación : 20-mar-2017
Editorial : Biomed Central
Cita Bibliográfica: Sanmamed MF, Esteban E, Uriol E, Zarate R, Capelan M, Muriel C, et al. Epidermal growth factor receptor and epididymis invasion as prognostic biomarkers in clinical stage I testicular germ cell tumours. J Transl Med. 2017; 15(1):62.
Abstract: Background Inguinal orchiectomy is curative in 70–80% of clinical stage I testicular germ cell tumours (CS I TGCT). The identification of patients who are at low risk of relapse is critical to avoid unnecessary treatment. The aim of this study is to explore EGFR, hMLH-1/hMSH-2 and microsatellite instability (MSI) as potential prognostic factors of recurrence in CS I TGCT. Methods Fifty-six CS I TGCT patients who underwent inguinal orchiectomy were included in this study. We analysed the relationship between clinicopathological and molecular factors with survival. Analysis of hMLH1, hMSH2 and EGFR expression was carried out by immunohistochemistry. Methylation status of the hMLH1 promoter was determined by pyrosequencing analysis in selected cases. EGFR exons 19, 20, 21 were analysed by PCR labeled-fragments and MSI status was determined using standard Multiplex MSI assays. Results Classical pathological factors such as lymphovascular invasion, high percentage of embryonal carcinoma, rete testis invasion or tumour size ≥4 cm showed a significant relationship with a higher risk of relapse. Additionally, it was found that an epididymis invasion proved to be a significant independent poor prognostic factor of recurrence (p = 0.001). hMLH1 or hMSH2 expression showed no significant association with risk of relapse and no MSI was found. EGFR expression was observed in 30.4% of samples and its expression was associated with higher risk of relapse (HR 3.5; 95% CI 1.3–9.8; p = 0.016). None of the cases presented EGFR kinase domain mutations. Conclusions Epididymis invasion and EGFR expression, but not hMLH-1/hMSH-2 or MSI, could be potentially useful as new prognostic factors of recurrence for CS I TGCT.
URI: http://hdl.handle.net/10668/2662
Versión del editor : https://translational-medicine.biomedcentral.com/articles/10.1186/s12967-017-1162-3#Abs1
DOI: 10.1186/s12967-017-1162-3
ISSN : 1479-5876 (Online)
Aparece en las colecciones: 01- Artículos - Hospital Regional de Málaga

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