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http://hdl.handle.net/10668/19504
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DC Field | Value | Language |
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dc.contributor.author | San Martin-Alonso, Marta | |
dc.contributor.author | Soler-Oliva, María E | |
dc.contributor.author | García-Rubio, María | |
dc.contributor.author | García-Muse, Tatiana | |
dc.contributor.author | Aguilera, Andrés | |
dc.date.accessioned | 2023-02-12T02:34:43Z | - |
dc.date.available | 2023-02-12T02:34:43Z | - |
dc.date.issued | 2021-07-22 | |
dc.identifier.uri | http://hdl.handle.net/10668/19504 | - |
dc.description.abstract | Identifying how R-loops are generated is crucial to know how transcription compromises genome integrity. We show by genome-wide analysis of conditional yeast mutants that the THO transcription complex, prevents R-loop formation in G1 and S-phase, whereas the Sen1 DNA-RNA helicase prevents them only in S-phase. Interestingly, damage accumulates asymmetrically downstream of the replication fork in sen1 cells but symmetrically in the hpr1 THO mutant. Our results indicate that: R-loops form co-transcriptionally independently of DNA replication; that THO is a general and cell-cycle independent safeguard against R-loops, and that Sen1, in contrast to previously believed, is an S-phase-specific R-loop resolvase. These conclusions have important implications for the mechanism of R-loop formation and the role of other factors reported to affect on R-loop homeostasis. | |
dc.language.iso | en | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject.mesh | Cell Cycle | |
dc.subject.mesh | DNA Damage | |
dc.subject.mesh | DNA Helicases | |
dc.subject.mesh | DNA, Fungal | |
dc.subject.mesh | Genes, Fungal | |
dc.subject.mesh | Genomic Instability | |
dc.subject.mesh | Models, Biological | |
dc.subject.mesh | Mutation | |
dc.subject.mesh | Nuclear Proteins | |
dc.subject.mesh | R-Loop Structures | |
dc.subject.mesh | RNA Helicases | |
dc.subject.mesh | RNA, Fungal | |
dc.subject.mesh | Saccharomyces cerevisiae | |
dc.subject.mesh | Saccharomyces cerevisiae Proteins | |
dc.subject.mesh | Transcription Factors | |
dc.title | Harmful R-loops are prevented via different cell cycle-specific mechanisms. | |
dc.type | research article | |
dc.identifier.pmid | 34294712 | |
dc.rights.accessRights | open access | |
dc.identifier.doi | 10.1038/s41467-021-24737-x | |
dc.identifier.essn | 2041-1723 | |
dc.identifier.pmc | PMC8298424 | |
dc.identifier.unpaywallURL | https://www.nature.com/articles/s41467-021-24737-x.pdf | |
dc.issue.number | 1 | |
dc.journal.title | Nature communications | |
dc.journal.titleabbreviation | Nat Commun | |
dc.organization | Centro Andaluz de Biología Molecular y Medicina Regenerativa-CABIMER | |
dc.page.number | 4451 | |
dc.pubmedtype | Journal Article | |
dc.pubmedtype | Research Support, Non-U.S. Gov't | |
dc.volume.number | 12 | |
dc.type.hasVersion | VoR | |
dc.identifier.pubmedURL | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8298424/pdf | |
Appears in Collections: | Producción 2020 |
Files in This Item:
File | Size | Format | |
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PMC8298424.pdf | 2,32 MB | Adobe PDF | View/Open |
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