Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/19504
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dc.contributor.authorSan Martin-Alonso, Marta
dc.contributor.authorSoler-Oliva, María E
dc.contributor.authorGarcía-Rubio, María
dc.contributor.authorGarcía-Muse, Tatiana
dc.contributor.authorAguilera, Andrés
dc.date.accessioned2023-02-12T02:34:43Z-
dc.date.available2023-02-12T02:34:43Z-
dc.date.issued2021-07-22
dc.identifier.urihttp://hdl.handle.net/10668/19504-
dc.description.abstractIdentifying how R-loops are generated is crucial to know how transcription compromises genome integrity. We show by genome-wide analysis of conditional yeast mutants that the THO transcription complex, prevents R-loop formation in G1 and S-phase, whereas the Sen1 DNA-RNA helicase prevents them only in S-phase. Interestingly, damage accumulates asymmetrically downstream of the replication fork in sen1 cells but symmetrically in the hpr1 THO mutant. Our results indicate that: R-loops form co-transcriptionally independently of DNA replication; that THO is a general and cell-cycle independent safeguard against R-loops, and that Sen1, in contrast to previously believed, is an S-phase-specific R-loop resolvase. These conclusions have important implications for the mechanism of R-loop formation and the role of other factors reported to affect on R-loop homeostasis.
dc.language.isoen
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.meshCell Cycle
dc.subject.meshDNA Damage
dc.subject.meshDNA Helicases
dc.subject.meshDNA, Fungal
dc.subject.meshGenes, Fungal
dc.subject.meshGenomic Instability
dc.subject.meshModels, Biological
dc.subject.meshMutation
dc.subject.meshNuclear Proteins
dc.subject.meshR-Loop Structures
dc.subject.meshRNA Helicases
dc.subject.meshRNA, Fungal
dc.subject.meshSaccharomyces cerevisiae
dc.subject.meshSaccharomyces cerevisiae Proteins
dc.subject.meshTranscription Factors
dc.titleHarmful R-loops are prevented via different cell cycle-specific mechanisms.
dc.typeresearch article
dc.identifier.pmid34294712
dc.rights.accessRightsopen access
dc.identifier.doi10.1038/s41467-021-24737-x
dc.identifier.essn2041-1723
dc.identifier.pmcPMC8298424
dc.identifier.unpaywallURLhttps://www.nature.com/articles/s41467-021-24737-x.pdf
dc.issue.number1
dc.journal.titleNature communications
dc.journal.titleabbreviationNat Commun
dc.organizationCentro Andaluz de Biología Molecular y Medicina Regenerativa-CABIMER
dc.page.number4451
dc.pubmedtypeJournal Article
dc.pubmedtypeResearch Support, Non-U.S. Gov't
dc.volume.number12
dc.type.hasVersionVoR
dc.identifier.pubmedURLhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8298424/pdf
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