Por favor, usa este identificador para citar o enlazar este ítem:
http://hdl.handle.net/10668/19504
Título : | Harmful R-loops are prevented via different cell cycle-specific mechanisms. |
Autor : | San Martin-Alonso, Marta Soler-Oliva, María E García-Rubio, María García-Muse, Tatiana Aguilera, Andrés |
MeSH: | Cell Cycle DNA Damage DNA Helicases DNA, Fungal Genes, Fungal Genomic Instability Models, Biological Mutation Nuclear Proteins R-Loop Structures RNA Helicases RNA, Fungal Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors |
Fecha de publicación : | 22-jul-2021 |
Abstract: | Identifying how R-loops are generated is crucial to know how transcription compromises genome integrity. We show by genome-wide analysis of conditional yeast mutants that the THO transcription complex, prevents R-loop formation in G1 and S-phase, whereas the Sen1 DNA-RNA helicase prevents them only in S-phase. Interestingly, damage accumulates asymmetrically downstream of the replication fork in sen1 cells but symmetrically in the hpr1 THO mutant. Our results indicate that: R-loops form co-transcriptionally independently of DNA replication; that THO is a general and cell-cycle independent safeguard against R-loops, and that Sen1, in contrast to previously believed, is an S-phase-specific R-loop resolvase. These conclusions have important implications for the mechanism of R-loop formation and the role of other factors reported to affect on R-loop homeostasis. |
URI: | http://hdl.handle.net/10668/19504 |
DOI: | 10.1038/s41467-021-24737-x |
Aparece en las colecciones: | Producción 2020 |
Ficheros en este ítem:
Fichero | Tamaño | Formato | |
---|---|---|---|
PMC8298424.pdf | 2,32 MB | Adobe PDF | Visualizar/Abrir |
Este ítem está protegido por copyright original |
Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons