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dc.contributor.authorGarrido-Sánchez, Lourdes-
dc.contributor.authorEscote, Xavier-
dc.contributor.authorCoín-Aragüez, Leticia-
dc.contributor.authorFernández-García, José Carlos-
dc.contributor.authorEl Bekay, Rajaa-
dc.contributor.authorVendrell, Joan-
dc.contributor.authorGarcía-Fuentes, Eduardo-
dc.contributor.authorTinahones, Francisco J-
dc.identifier.citationGarrido-Sanchez L, Escote X, Coin-Aragüez L, Fernandez-Garcia JC, El Bekay R, Vendrell J, et al. Munc18c in adipose tissue is downregulated in obesity and is associated with insulin. PLoS ONE. 2013; 8(5):e63937es
dc.identifier.issn1932-6203 (Online)-
dc.descriptionJournal Article;es
dc.description.abstractOBJECTIVE Munc18c is associated with glucose metabolism and could play a relevant role in obesity. However, little is known about the regulation of Munc18c expression. We analyzed Munc18c gene expression in human visceral (VAT) and subcutaneous (SAT) adipose tissue and its relationship with obesity and insulin. MATERIALS AND METHODS We evaluated 70 subjects distributed in 12 non-obese lean subjects, 23 overweight subjects, 12 obese subjects and 23 nondiabetic morbidly obese patients (11 with low insulin resistance and 12 with high insulin resistance). RESULTS The lean, overweight and obese persons had a greater Munc18c gene expression in adipose tissue than the morbidly obese patients (p<0.001). VAT Munc18c gene expression was predicted by the body mass index (B = -0.001, p = 0.009). In SAT, no associations were found by different multiple regression analysis models. SAT Munc18c gene expression was the main determinant of the improvement in the HOMA-IR index 15 days after bariatric surgery (B = -2148.4, p = 0.038). SAT explant cultures showed that insulin produced a significant down-regulation of Munc18c gene expression (p = 0.048). This decrease was also obtained when explants were incubated with liver X receptor alpha (LXRα) agonist, either without (p = 0.038) or with insulin (p = 0.050). However, Munc18c gene expression was not affected when explants were incubated with insulin plus a sterol regulatory element-binding protein-1c (SREBP-1c) inhibitor (p = 0.504). CONCLUSIONS Munc18c gene expression in human adipose tissue is down-regulated in morbid obesity. Insulin may have an effect on the Munc18c expression, probably through LXRα and
dc.description.sponsorshipThis work was supported in part by grants from Instituto de Salud Carlos III [CP04/00133, PS09/01060, PS09/00997], Servicio Andaluz de Salud [PI0255/2007]. L. Garrido-Sánchez is supported by a fellowship from the Programa Juan de la Cierva [JCI-2009-04086]. E. Garcia-Fuentes is supported by the Research Stabilization Program of the Instituto de Salud Carlos III (ISCIII). R. El Bekay is supported by fellowships from the Fondo de Investigación Sanitaria (FIS) "Miguel Servet" [CP07/00288].es
dc.publisherPublic Library of Sciencees
dc.relation.ispartofPloS onees
dc.subjectResistencia a la Insulinaes
dc.subjectObesidad Mórbidaes
dc.subjectTejido Adiposoes
dc.subjectRegulación de la Expresión Génicaes
dc.subjectProteínas Munc18es
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Physiological Phenomena::Pharmacological Phenomena::Drug Resistance::Insulin Resistancees
dc.subject.meshMedical Subject Headings::Diseases::Nutritional and Metabolic Diseases::Nutrition Disorders::Overnutrition::Obesity::Obesity, Morbides
dc.subject.meshMedical Subject Headings::Anatomy::Tissues::Connective Tissue::Adipose Tissuees
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Carbohydrates::Monosaccharides::Hexoses::Glucosees
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Lipids::Lipoproteins::Lipoproteins, HDL::Cholesterol, HDLes
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression Regulationes
dc.subject.meshMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humanses
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Cohort Studies::Longitudinal Studies::Prospective Studieses
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Membrane Proteins::Vesicular Transport Proteins::Munc18 Proteinses
dc.titleMunc18c in adipose tissue is downregulated in obesity and is associated with
dc.rights.accessRightsAcceso abiertoes
dc.contributor.authoraffiliation[Garrido-Sánchez,L; Escote,X; Vendrell,J] Endocrinology and Diabetes Unit, Joan XXIII University Hospital, IISPV, Universitat Rovira i Virgili, Tarragona, Spain. [Garrido-Sánchez,L; Escote,X; Vendrell,J] CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Instituto de Salud Carlos III, Tarragona, Spain. [Coín-Aragüez,L; Fernández-García,JC; El Bekay,R; Tinahones,FJ] Servicio de Endocrinología y Nutrición, Hospital Universitario Virgen de la Victoria, Málaga, Spain. [Coín-Aragüez,L; El Bekay,R; García-Fuentes,E; Tinahones,FJ] Ciber Fisiopatología Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Málaga, Spain. [El Bekay,R; García-Fuentes,E] Instituto de Investigaciones Biomédicas de Málaga (IBIMA), Málaga, Spain. [García-Fuentes,E] Servicio de Endocrinología y Nutrición, Hospital Regional Universitario Carlos Haya, Málaga,
Appears in Collections:01- Artículos - Hospital Regional de Málaga
01- Artículos - Hospital Virgen de la Victoria
01- Artículos - IBIMA. Instituto de Investigación Biomédica de Málaga

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