Publication: Use of MALDI-TOF MS for Identification of Nontuberculous Mycobacterium Species Isolated from Clinical Specimens.
| dc.contributor.author | Mediavilla-Gradolph, María Concepción | |
| dc.contributor.author | De Toro-Peinado, Inmaculada | |
| dc.contributor.author | Bermúdez-Ruiz, María Pilar | |
| dc.contributor.author | García-Martínez, María de los Ángeles | |
| dc.contributor.author | Ortega-Torres, María | |
| dc.contributor.author | Montiel Quezel-Guerraz, Natalia | |
| dc.contributor.author | Palop-Borrás, Begoña | |
| dc.contributor.authoraffiliation | [Mediavilla-Gradolph,MC; De Toro-Peinado,I; Bermúdez-Ruiz,MP; García-Martínez,MA; Ortega-Torres,M; Palop-Borrás,B] Infectious Diseases and Microbiology Service, Regional University and Virgen de la Victoria University Hospitals, IBIMA, Malaga, Spain. [Montiel Quezel-Guerraz,N] Microbiology Unit, Hospital Costa del Sol, Marbella, Spain. | es |
| dc.date.accessioned | 2016-06-29T11:01:17Z | |
| dc.date.available | 2016-06-29T11:01:17Z | |
| dc.date.issued | 2015 | |
| dc.description | Journal Article; | es |
| dc.description.abstract | The aim of this study was to compare the results obtained for identification by MALDI-TOF of nontuberculous mycobacteria (NTM) isolated in clinical samples with those obtained by GenoType Mycobacterium CM/AS (common mycobacteria/additional species). A total of 66 Mycobacterium isolates from various clinical specimens (mainly respiratory) were tested in this study. They were identified using MALDI-TOF Bruker from strains isolated in Lowenstein, following the recommended protocol of heat inactivation and extraction, and were simultaneously analyzed through hybridization by GenoType Mycobacterium from liquid culture MGIT. Our results showed that identification by MALDI-TOF was correct in 98.4% (65/66) of NTM isolated in our clinical practice (M. avium, M. intracellulare, M. abscessus, M. chelonae, M. fortuitum, M. mucogenicum, M. kansasii, and M. scrofulaceum). MALDI-TOF was found to be an accurate, rapid, and cost-effective system for identification of mycobacteria species. | es |
| dc.description.version | Yes | es |
| dc.identifier.citation | Mediavilla-Gradolph MC, De Toro-Peinado I, Bermúdez-Ruiz MP, García-Martínez Mde L, Ortega-Torres M, Montiel Quezel-Guerraz N, et al. Use of MALDI-TOF MS for Identification of Nontuberculous Mycobacterium Species Isolated from Clinical Specimens. Biomed Res Int. 2015; 2015:854078 | es |
| dc.identifier.doi | 10.1155/2015/854078 | |
| dc.identifier.essn | 2314-6141 | |
| dc.identifier.pmc | PMC4463989 | |
| dc.identifier.pmid | 26106617 | |
| dc.identifier.uri | http://hdl.handle.net/10668/2240 | |
| dc.journal.title | BioMed Research International | |
| dc.language.iso | en | |
| dc.publisher | Hindawi Publishing Corporation | es |
| dc.relation.publisherversion | http://www.hindawi.com/journals/bmri/2015/854078/abs/ | es |
| dc.rights.accessRights | open access | |
| dc.subject | Humanos | es |
| dc.subject | Genotipo | es |
| dc.subject | Infecciones por micobacterias no tuberculosas | es |
| dc.subject | Micobacterias no tuberculosas | es |
| dc.subject | Espectrometría de masas con desorción-ionización láser asistida por matriz | es |
| dc.subject.mesh | Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans | es |
| dc.subject.mesh | Medical Subject Headings::Diseases::Bacterial Infections and Mycoses::Bacterial Infections::Gram-Positive Bacterial Infections::Actinomycetales Infections::Mycobacterium Infections::Mycobacterium Infections, Nontuberculous | es |
| dc.subject.mesh | Medical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Actinobacteria::Actinomycetales::Mycobacteriaceae::Mycobacterium::Nontuberculous Mycobacteria | es |
| dc.subject.mesh | Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Mass Spectrometry::Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | es |
| dc.subject.mesh | Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genotype | es |
| dc.title | Use of MALDI-TOF MS for Identification of Nontuberculous Mycobacterium Species Isolated from Clinical Specimens. | es |
| dc.type | research article | |
| dc.type.hasVersion | VoR | |
| dspace.entity.type | Publication |
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