Publication:
A Parasite Biomarker Set for Evaluating Benznidazole Treatment Efficacy in Patients with Chronic Asymptomatic Trypanosoma cruzi Infection.

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2019-09-23

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Egui, Adriana
Thomas, M Carmen
Fernández-Villegas, Ana
Pérez-Antón, Elena
Gómez, Inmaculada
Carrilero, Bartolomé
Del Pozo, Ángel
Ceballos, Maialen
Andrés-León, Eduardo
López-Ruz, Miguel Ángel

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Abstract

One of the current greatest challenges of Chagas disease is the establishment of biomarkers to assess the efficacy of drugs in a short period of time. In this context, the reactivity of sera from 66 adults with chronic indeterminate Chagas disease (IND) for a set of four Trypanosoma cruzi antigens (KMP11, PFR2, HSP70, and 3973d) was analyzed before and after benznidazole treatment. The results showed that the reactivity against these antigens decreased at 9, 24, and 48 months after treatment. Moreover, the 42.4% and 68.75% of IND patients met the established standard criteria of therapeutic efficacy (STEC) at 24 and 48 months posttreatment, respectively. Meeting the STEC implied that there was a continuous decrease in the reactivity of the patient sera against the four antigens after treatment and that there was a substantial decrease in the reactivity for at least two of the antigens. This important decrease in reactivity may be associated with a drastic reduction in the parasite load, but it is not necessarily associated with a parasitological cure. After treatment, a positive PCR result was only obtained in patients who did not meet the STEC. The percentage of granzyme B+/perforin+ CD8+ T cells was significantly higher in patients who met the STEC than in those who did not meet the STEC (35.2% versus 2.2%; P 

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Adult
Biomarkers
CD8-Positive T-Lymphocytes
Chagas Disease
Cytokines
Enzyme-Linked Immunosorbent Assay
Female
Granzymes
Humans
Male
Middle Aged
Nitroimidazoles
Perforin
Polymerase Chain Reaction
Trypanocidal Agents
Trypanosoma cruzi
Young Adult

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Keywords

Chagas disease, Trypanosoma cruzi, antibodies, benznidazole, biomarkers, cellular response, cytokines, cytotoxic molecules, seroconversion, therapeutic response

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