Strategy for optimizing DNA amplification in a peripheral blood PCR assay used for diagnosis of human brucellosis.

Morata, Pilar; Queipo-Ortuño, María Isabel; de Dios Colmenero, Juan de Dios

Publication:
Strategy for optimizing DNA amplification in a peripheral blood PCR assay used for diagnosis of human brucellosis.

dc.contributor.author	Morata, Pilar
dc.contributor.author	Queipo-Ortuño, María Isabel
dc.contributor.author	de Dios Colmenero, Juan de Dios
dc.contributor.authoraffiliation	[Morata,P; Queipo-Ortuño,MI] Department of Biochemistry and Molecular Biology, Málaga, University, Málaga, Spain. [Colmenero,J de D] Infectious Diseases Unit, "CarlosHaya" Regional Hospital, Málaga, Spain.	es
dc.contributor.funder	This research was supported by FIS grant 97/0713 and funds from PAI 54/97 Consejeria de Salud, Junta de Andalucia, Spain.
dc.date.accessioned	2016-03-02T09:53:41Z
dc.date.available	2016-03-02T09:53:41Z
dc.date.issued	1998-09
dc.description	Journal Article; Research Support, Non-U.S. Gov't;	es
dc.description.abstract	We studied two of the possible factors which can interfere with specific DNA amplification in a peripheral-blood PCR assay used for the diagnosis of human brucellosis. We found that high concentrations of leukocyte DNA and heme compounds inhibit PCR. These inhibitors can be efficiently suppressed by increasing the number of washings to four or five and decreasing the amount of total DNA to 2 to 4 microg, thereby avoiding false-negative results.	es
dc.description.version	Yes	es
dc.identifier.citation	Morata P, Queipo-Ortuño MI, Colmenero J de D. Strategy for optimizing DNA amplification in a peripheral blood PCR assay used for diagnosis of human brucellosis. J Clin Microbiol. 1998; 36(9):2443-6	es
dc.identifier.essn	1098-660X
dc.identifier.issn	0095-1137
dc.identifier.pmc	PMC105141
dc.identifier.pmid	9705371
dc.identifier.uri	http://hdl.handle.net/10668/2146
dc.journal.title	Journal of Clinical Microbiology
dc.language.iso	en
dc.publisher	American Society for Microbiology	es
dc.relation.publisherversion	http://jcm.asm.org/content/36/9/2443.abstract	es
dc.rights.accessRights	open access
dc.subject	España	es
dc.subject	Brucelosis	es
dc.subject	ADN bacteriano	es
dc.subject	Electroforesis en gel de agar	es
dc.subject	Leucocitos	es
dc.subject	Reacción en cadena de la polimerasa	es
dc.subject.mesh	Medical Subject Headings::Organisms::Bacteria::Gram-Negative Bacteria::Gram-Negative Aerobic Bacteria::Gram-Negative Aerobic Rods and Cocci::Brucellaceae::Brucella::Brucella abortus	es
dc.subject.mesh	Medical Subject Headings::Diseases::Bacterial Infections and Mycoses::Bacterial Infections::Gram-Negative Bacterial Infections::Brucellosis	es
dc.subject.mesh	Medical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::DNA::DNA, Bacterial	es
dc.subject.mesh	Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Electrophoresis::Electrophoresis, Agar Gel	es
dc.subject.mesh	Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans	es
dc.subject.mesh	Medical Subject Headings::Anatomy::Hemic and Immune Systems::Blood::Blood Cells::Leukocytes	es
dc.subject.mesh	Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction	es
dc.subject.mesh	Medical Subject Headings::Geographicals::Geographic Locations::Europe::Spain	es
dc.subject.mesh	Medical Subject Headings::Organisms::Bacteria::Gram-Negative Bacteria::Gram-Negative Aerobic Bacteria::Gram-Negative Aerobic Rods and Cocci::Brucellaceae::Brucella	es
dc.title	Strategy for optimizing DNA amplification in a peripheral blood PCR assay used for diagnosis of human brucellosis.	es
dc.type	research article
dc.type.hasVersion	VoR
dspace.entity.type	Publication

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Publication: Strategy for optimizing DNA amplification in a peripheral blood PCR assay used for diagnosis of human brucellosis.

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Publication:
Strategy for optimizing DNA amplification in a peripheral blood PCR assay used for diagnosis of human brucellosis.