Publication: A novel capillary nano-immunoassay for assessing androgen receptor splice variant 7 in plasma. Correlation with CD133 antigen expression in circulating tumor cells. A pilot study in prostate cancer patients.
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Date
2017-06-09
Authors
Garcia, J L
Lozano, R
Misiewicz-Krzeminska, I
Fernandez-Mateos, J
Krzeminski, P
Alfonso, S
Marcos, R A
Garcia, R
Gomez-Veiga, F
Virseda, A
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Publisher
Springer
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Abstract
Androgen receptor (AR) splice variant 7 (AR-V7) has been related with both a higher risk of prostate cancer (PC) progression and differential responsiveness to hormonal agents versus chemotherapy. The objective of this study was to investigate the feasibility of a novel capillary nano-immunoassay in assessing AR-V7 in plasma from PC patients. Patients with either localized or advanced PC were included in the study. Assessment of AR-V7 in plasma was performed through a capillary nano-immunoassay platform. Correlation with clinical data, stem cell biomarkers (such as CD133+), AR amplification and PTEN status was identified. The study included 72 PC patients. AR-V7 signal was detected in 21 (29%) patients: 17 (81%) had a Gleason score ≥7, 15 (71%) castration-resistant prostate cancer (CRPC), 18 (86%) metastatic disease and PSA (median) high than AR-V7 negative (p Assessing the presence of AR-V7 in plasma from PC patients is feasible by a novel capillary nano-immunoassay. AR-V7 was observed in 29% of the tumors and is more frequent in aggressive tumors.
Description
MeSH Terms
AC133 Antigen
Alternative Splicing
Biomarkers, Tumor
Follow-Up Studies
Humans
Immunoassay
Male
Nanomedicine
Neoplastic Cells, Circulating
Pilot Projects
Prognosis
Prostatic Neoplasms
Receptors, Androgen
Alternative Splicing
Biomarkers, Tumor
Follow-Up Studies
Humans
Immunoassay
Male
Nanomedicine
Neoplastic Cells, Circulating
Pilot Projects
Prognosis
Prostatic Neoplasms
Receptors, Androgen
DeCS Terms
Inmunoensayo
Capilares
Plasma
Neoplasias de la próstata
Receptores androgénicos
Biomarcadores
Castración
Capilares
Plasma
Neoplasias de la próstata
Receptores androgénicos
Biomarcadores
Castración
CIE Terms
Keywords
AR-V7, Androgen receptor, CD133, Capillary nano-immunoassay, Circulating tumor cells, PTEN
Citation
García JL, Lozano R, Misiewicz-Krzeminska I, Fernández-Mateos J, Krzeminski P, Alfonso S, et al. A novel capillary nano-immunoassay for assessing androgen receptor splice variant 7 in plasma. Correlation with CD133 antigen expression in circulating tumor cells. A pilot study in prostate cancer patients. Clin Transl Oncol. 2017 Nov;19(11):1350-1357