Publication: Effect of Quercetin on Hepatitis C Virus Life Cycle: From Viral to Host Targets.
| dc.contributor.author | Rojas, Ángela | |
| dc.contributor.author | Del Campo, Jose A | |
| dc.contributor.author | Clement, Sophie | |
| dc.contributor.author | Lemasson, Matthieu | |
| dc.contributor.author | García-Valdecasas, Marta | |
| dc.contributor.author | Gil-Gómez, Antonio | |
| dc.contributor.author | Ranchal, Isidora | |
| dc.contributor.author | Bartosch, Birke | |
| dc.contributor.author | Bautista, Juan D | |
| dc.contributor.author | Rosenberg, Arielle R | |
| dc.contributor.author | Negro, Francesco | |
| dc.contributor.author | Romero-Gómez, Manuel | |
| dc.contributor.authoraffiliation | [Rojas,A; Garcia-Valdecasas,M; Gil-Gómez,A; Romero-Gómez,M] UCM Digestive Diseases, Virgen Macarena-Virgen del Rocío University Hospitals and CIBERehd, Institute of Biomedicine, University of Sevilla, Sevilla, Spain. [Rojas,A; Del Campo,JA; Garcia-Valdecasas,M; Gil-Gómez,A; Ranchal,I] Unit for the Clinical Management of Digestive Diseases, Hospital Universitario Valme de Sevilla, Sevilla, Spain. [Cement,S; Negro,F] Division of Clinical Pathology, University Hospital, Geneva, Switzerland. [Lemasson,M; Rosenberg,AR] University Paris Descartes, EA 4474 "Hepatitis C Virology", France. [Bartosch,B] Inserm U1052, Cancer Research Centre, University of Lyon, France DevWeCan Laboratories of Excellence Network (Labex), Lyon, France. [Bautista,JD] Biochemistry and Molecular Biology, Faculty of Pharmacy, University of Sevilla, Spain. [Negro,F] Division of Gastroenterology and Hepatology, University Hospital, Geneva, Switzerland. | es |
| dc.contributor.funder | This research was supported by the Spanish Ministry of Economy, Innovation and Competition, Instituto de Salud Carlos III PI10/00611, PI13/01192 and by the Government of Andalusia (PI-0892-2012). We thank to AEEH, which awarded a postdoctoral research fellowship to ÁR. | |
| dc.date.accessioned | 2016-10-25T07:50:55Z | |
| dc.date.available | 2016-10-25T07:50:55Z | |
| dc.date.issued | 2016-08-22 | |
| dc.description | Journal Article; | es |
| dc.description.abstract | Quercetin is a natural flavonoid, which has been shown to have anti hepatitis C virus (HCV) properties. However, the exact mechanisms whereby quercetin impacts the HCV life cycle are not fully understood. We assessed the effect of quercetin on different steps of the HCV life cycle in Huh-7.5 cells and primary human hepatocytes (PHH) infected with HCVcc. In both cell types, quercetin significantly decreased i) the viral genome replication; ii) the production of infectious HCV particles and iii) the specific infectivity of the newly produced viral particles (by 85% and 92%, Huh7.5 and PHH respectively). In addition, when applied directly on HCV particles, quercetin reduced their infectivity by 65%, suggesting that it affects the virion integrity. Interestingly, the HCV-induced up-regulation of diacylglycerol acyltransferase (DGAT) and the typical localization of the HCV core protein to the surface of lipid droplets, known to be mediated by DGAT, were both prevented by quercetin. In conclusion, quercetin appears to have direct and host-mediated antiviral effects against HCV. | es |
| dc.description.version | Yes | es |
| dc.identifier.citation | Rojas Á, Del Campo JA, Clement S, Lemasson M, García-Valdecasas M, Gil-Gómez A, et al. Effect of Quercetin on Hepatitis C Virus Life Cycle: From Viral to Host Targets. Sci Rep. 2016 ; 6:31777 | es |
| dc.identifier.doi | 10.1038/srep31777 | |
| dc.identifier.essn | 2045-2322 | |
| dc.identifier.pmc | PMC4992894 | |
| dc.identifier.pmid | 27546480 | |
| dc.identifier.uri | http://hdl.handle.net/10668/2493 | |
| dc.journal.title | Scientific Reports | |
| dc.language.iso | en | |
| dc.publisher | Thieme Publishing | es |
| dc.relation.publisherversion | http://www.nature.com/articles/srep31777 | es |
| dc.rights.accessRights | open access | |
| dc.subject | Antivirales | es |
| dc.subject | Diacilglicerol o-acetiltransferasa | es |
| dc.subject | Genoma viral | es |
| dc.subject | Hepacivirus | es |
| dc.subject | Hepatitis C | es |
| dc.subject | Quercetina | es |
| dc.subject | Regulación hacia arriba | es |
| dc.subject.mesh | Medical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Anti-Infective Agents::Antiviral Agents | es |
| dc.subject.mesh | Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Transferases::Acyltransferases::Diacylglycerol O-Acyltransferase | es |
| dc.subject.mesh | Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Structures::Genome::Genome, Viral | es |
| dc.subject.mesh | Medical Subject Headings::Organisms::Viruses::Hepatitis Viruses::Hepacivirus | es |
| dc.subject.mesh | Medical Subject Headings::Diseases::Virus Diseases::Hepatitis, Viral, Human::Hepatitis C | es |
| dc.subject.mesh | Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans | es |
| dc.subject.mesh | Medical Subject Headings::Chemicals and Drugs::Heterocyclic Compounds::Heterocyclic Compounds, 2-Ring::Benzopyrans::Chromones::Flavonoids::Flavonols::Quercetin | es |
| dc.subject.mesh | Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::Up-Regulation | es |
| dc.subject.mesh | Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Viral Proteins::Viral Structural Proteins::Nucleocapsid Proteins::Viral Core Proteins | es |
| dc.subject.mesh | Medical Subject Headings::Anatomy::Viral Structures::Virion | es |
| dc.subject.mesh | Medical Subject Headings::Phenomena and Processes::Microbiological Phenomena::Microbiological Processes::Virus Physiological Processes::Virus Replication | es |
| dc.title | Effect of Quercetin on Hepatitis C Virus Life Cycle: From Viral to Host Targets. | es |
| dc.type | research article | |
| dc.type.hasVersion | VoR | |
| dspace.entity.type | Publication |