Publication:
Point-of-care detection of SARS-CoV-2 antigen among symptomatic vs. asymptomatic persons: Testing for COVID-19 vs. infectivity.

dc.contributor.authorNeukam, Karin
dc.contributor.authorLucero, Alicia
dc.contributor.authorGutierrez-Valencia, Alicia
dc.contributor.authorAmaya, Lucas
dc.contributor.authorEchegoyen, Natalia
dc.contributor.authorMartelli, Antonella
dc.contributor.authorVidela, Cristina
dc.contributor.authorDi-Lello, Federico A
dc.contributor.authorMartinez, Alfredo P
dc.date.accessioned2023-05-03T13:45:36Z
dc.date.available2023-05-03T13:45:36Z
dc.date.issued2022-10-17
dc.description.abstract Management of the coronavirus disease 2019 (COVID-19) pandemic caused by a novel severe acute respiratory syndrome coronavirus-2 ( SARS-CoV-2 ) requires rapid and simple methods to detect COVID-19 patients and identify potential infectors. This study aimed to evaluate the utility of a point-of-care (PoC) rapid antigen diagnostic test (Ag-RDT) in these settings. Patients and methods : Individuals who consecutively presented for SARS-CoV-2 testing at a tertiary care center in Buenos Aires, Argentina , underwent PoC Ag-RDT testing and real- time RT-PCR (qRT- PCR ) on the same day during June 2021.Results: Of 584 included subjects, 108 (18.5%) were symptomatic for COVID-19 while the remaining presented for miscellaneous reasons unrelated to possible or confirmed contact with a SARS-CoV-2 -infected individual. A positive Ag-RDT result was obtained in 26 (24.1%) symptomatic and 7 (1.5%) asymptomatic persons (p < 0.001), which was concordant with qRT- PCR in 105/108 [97.2%, Cohen's kappa coefficient (κ) = 0.927] symptomatic and 467/476 (98.1% κ = 0.563) asymptomatic participants, with a positive percentage agreement (PPA; 95% confidence interval ) of 89.7% (71.5-97.3%) and 42.9% (18.8-70.4%), respectively. None of the 11 false-negative diagnoses showed a Ct-value ≤20. Considering only failures with a Ct-value below 31 as hypothetical infectivity threshold of 105 SARS-CoV-2 RNA copies/mL, concordance was observed in 98.1% (κ = 0.746) in the asymptomatic population , accounting for a PPA of 66.7% (30.9-91%).Conclusions: PoC Ag-RDT accurately detected active SARS-CoV-2 infection and showed acceptable diagnostic performance in asymptomatic persons potentially spreading infectious virus . Ag-RDT may therefore be useful to slow down or stop transmission by enabling adequate decisions on isolation at a public health level.
dc.description.versionSi
dc.identifier.citationNeukam K, Lucero A, Gutiérrez-Valencia A, Amaya L, Echegoyen N, Martelli A, et al. Point-of-care detection of SARS-CoV-2 antigen among symptomatic vs. asymptomatic persons: Testing for COVID-19 vs. infectivity. Front Public Health. 2022 Oct 17;10:995249.
dc.identifier.doi10.3389/fpubh.2022.995249
dc.identifier.essn2296-2565
dc.identifier.pmcPMC9619045
dc.identifier.pmid36324442
dc.identifier.pubmedURLhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9619045/pdf
dc.identifier.unpaywallURLhttps://www.frontiersin.org/articles/10.3389/fpubh.2022.995249/pdf
dc.identifier.urihttp://hdl.handle.net/10668/20741
dc.journal.titleFrontiers in public health
dc.journal.titleabbreviationFront Public Health
dc.language.isoen
dc.organizationHospital Universitario Virgen del Rocío
dc.organizationInstituto de Biomedicina de Sevilla-IBIS
dc.page.number8
dc.provenanceRealizada la curación de contenido 27/06/2025.
dc.publisherFrontiers Research Foundation
dc.pubmedtypeJournal Article
dc.pubmedtypeResearch Support, Non-U.S. Gov't
dc.relation.publisherversionhttps://doi.org/10.3389/fpubh.2022.995249
dc.rightsAttribution 4.0 International
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectCOVID-19
dc.subjectPCR
dc.subjectSARS-CoV-2
dc.subjectpoint-of-care
dc.subjectpublic health
dc.subjectrapid antigen testing
dc.subjectsurveillance
dc.subjectviral kinetics
dc.subject.decsSARS-CoV-2
dc.subject.decsMétodos
dc.subject.decsPandemias
dc.subject.decsDiagnóstico
dc.subject.decsAntígenos
dc.subject.decsIntervalos de confianza
dc.subject.decsPruebas diagnósticas de rutina
dc.subject.meshHumans
dc.subject.meshCOVID-19
dc.subject.meshSARS-CoV-2
dc.subject.meshCOVID-19 Testing
dc.subject.meshPoint-of-Care Systems
dc.subject.meshRNA, Viral
dc.subject.meshSensitivity and Specificity
dc.titlePoint-of-care detection of SARS-CoV-2 antigen among symptomatic vs. asymptomatic persons: Testing for COVID-19 vs. infectivity.
dc.typeresearch article
dc.type.hasVersionVoR
dc.volume.number10
dspace.entity.typePublication

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