Publication: Point-of-care detection of SARS-CoV-2 antigen among symptomatic vs. asymptomatic persons: Testing for COVID-19 vs. infectivity.
dc.contributor.author | Neukam, Karin | |
dc.contributor.author | Lucero, Alicia | |
dc.contributor.author | Gutierrez-Valencia, Alicia | |
dc.contributor.author | Amaya, Lucas | |
dc.contributor.author | Echegoyen, Natalia | |
dc.contributor.author | Martelli, Antonella | |
dc.contributor.author | Videla, Cristina | |
dc.contributor.author | Di-Lello, Federico A | |
dc.contributor.author | Martinez, Alfredo P | |
dc.date.accessioned | 2023-05-03T13:45:36Z | |
dc.date.available | 2023-05-03T13:45:36Z | |
dc.date.issued | 2022-10-17 | |
dc.description.abstract | Management of the coronavirus disease 2019 (COVID-19) pandemic caused by a novel severe acute respiratory syndrome coronavirus-2 ( SARS-CoV-2 ) requires rapid and simple methods to detect COVID-19 patients and identify potential infectors. This study aimed to evaluate the utility of a point-of-care (PoC) rapid antigen diagnostic test (Ag-RDT) in these settings. Patients and methods : Individuals who consecutively presented for SARS-CoV-2 testing at a tertiary care center in Buenos Aires, Argentina , underwent PoC Ag-RDT testing and real- time RT-PCR (qRT- PCR ) on the same day during June 2021.Results: Of 584 included subjects, 108 (18.5%) were symptomatic for COVID-19 while the remaining presented for miscellaneous reasons unrelated to possible or confirmed contact with a SARS-CoV-2 -infected individual. A positive Ag-RDT result was obtained in 26 (24.1%) symptomatic and 7 (1.5%) asymptomatic persons (p < 0.001), which was concordant with qRT- PCR in 105/108 [97.2%, Cohen's kappa coefficient (κ) = 0.927] symptomatic and 467/476 (98.1% κ = 0.563) asymptomatic participants, with a positive percentage agreement (PPA; 95% confidence interval ) of 89.7% (71.5-97.3%) and 42.9% (18.8-70.4%), respectively. None of the 11 false-negative diagnoses showed a Ct-value ≤20. Considering only failures with a Ct-value below 31 as hypothetical infectivity threshold of 105 SARS-CoV-2 RNA copies/mL, concordance was observed in 98.1% (κ = 0.746) in the asymptomatic population , accounting for a PPA of 66.7% (30.9-91%).Conclusions: PoC Ag-RDT accurately detected active SARS-CoV-2 infection and showed acceptable diagnostic performance in asymptomatic persons potentially spreading infectious virus . Ag-RDT may therefore be useful to slow down or stop transmission by enabling adequate decisions on isolation at a public health level. | |
dc.description.version | Si | |
dc.identifier.citation | Neukam K, Lucero A, Gutiérrez-Valencia A, Amaya L, Echegoyen N, Martelli A, et al. Point-of-care detection of SARS-CoV-2 antigen among symptomatic vs. asymptomatic persons: Testing for COVID-19 vs. infectivity. Front Public Health. 2022 Oct 17;10:995249. | |
dc.identifier.doi | 10.3389/fpubh.2022.995249 | |
dc.identifier.essn | 2296-2565 | |
dc.identifier.pmc | PMC9619045 | |
dc.identifier.pmid | 36324442 | |
dc.identifier.pubmedURL | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9619045/pdf | |
dc.identifier.unpaywallURL | https://www.frontiersin.org/articles/10.3389/fpubh.2022.995249/pdf | |
dc.identifier.uri | http://hdl.handle.net/10668/20741 | |
dc.journal.title | Frontiers in public health | |
dc.journal.titleabbreviation | Front Public Health | |
dc.language.iso | en | |
dc.organization | Hospital Universitario Virgen del Rocío | |
dc.organization | Instituto de Biomedicina de Sevilla-IBIS | |
dc.page.number | 8 | |
dc.provenance | Realizada la curación de contenido 27/06/2025. | |
dc.publisher | Frontiers Research Foundation | |
dc.pubmedtype | Journal Article | |
dc.pubmedtype | Research Support, Non-U.S. Gov't | |
dc.relation.publisherversion | https://doi.org/10.3389/fpubh.2022.995249 | |
dc.rights | Attribution 4.0 International | |
dc.rights.accessRights | open access | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | COVID-19 | |
dc.subject | PCR | |
dc.subject | SARS-CoV-2 | |
dc.subject | point-of-care | |
dc.subject | public health | |
dc.subject | rapid antigen testing | |
dc.subject | surveillance | |
dc.subject | viral kinetics | |
dc.subject.decs | SARS-CoV-2 | |
dc.subject.decs | Métodos | |
dc.subject.decs | Pandemias | |
dc.subject.decs | Diagnóstico | |
dc.subject.decs | Antígenos | |
dc.subject.decs | Intervalos de confianza | |
dc.subject.decs | Pruebas diagnósticas de rutina | |
dc.subject.mesh | Humans | |
dc.subject.mesh | COVID-19 | |
dc.subject.mesh | SARS-CoV-2 | |
dc.subject.mesh | COVID-19 Testing | |
dc.subject.mesh | Point-of-Care Systems | |
dc.subject.mesh | RNA, Viral | |
dc.subject.mesh | Sensitivity and Specificity | |
dc.title | Point-of-care detection of SARS-CoV-2 antigen among symptomatic vs. asymptomatic persons: Testing for COVID-19 vs. infectivity. | |
dc.type | research article | |
dc.type.hasVersion | VoR | |
dc.volume.number | 10 | |
dspace.entity.type | Publication |
Files
Original bundle
1 - 1 of 1