Publication:
Human Pluripotent Stem Cell Differentiation into Functional Epicardial Progenitor Cells.

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dc.contributor.authorGuadix, Juan Antonio
dc.contributor.authorOrlova, Valeria V
dc.contributor.authorGiacomelli, Elisa
dc.contributor.authorBellin, Milena
dc.contributor.authorRibeiro, Marcelo C
dc.contributor.authorMummery, Christine L
dc.contributor.authorPérez-Pomares, José M
dc.contributor.authorPassier, Robert
dc.date.accessioned2023-01-25T10:01:38Z
dc.date.available2023-01-25T10:01:38Z
dc.date.issued2017-11-22
dc.description.abstractHuman pluripotent stem cells (hPSCs) are widely used to study cardiovascular cell differentiation and function. Here, we induced differentiation of hPSCs (both embryonic and induced) to proepicardial/epicardial progenitor cells that cover the heart during development. Addition of retinoic acid (RA) and bone morphogenetic protein 4 (BMP4) promoted expression of the mesodermal marker PDGFRα, upregulated characteristic (pro)epicardial progenitor cell genes, and downregulated transcription of myocardial genes. We confirmed the (pro)epicardial-like properties of these cells using in vitro co-culture assays and in ovo grafting of hPSC-epicardial cells into chick embryos. Our data show that RA + BMP4-treated hPSCs differentiate into (pro)epicardial-like cells displaying functional properties (adhesion and spreading over the myocardium) of their in vivo counterpart. The results extend evidence that hPSCs are an excellent model to study (pro)epicardial differentiation into cardiovascular cells in human development and evaluate their potential for cardiac regeneration.
dc.identifier.doi10.1016/j.stemcr.2017.10.023
dc.identifier.essn2213-6711
dc.identifier.pmcPMC5785703
dc.identifier.pmid29173898
dc.identifier.pubmedURLhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5785703/pdf
dc.identifier.unpaywallURLhttp://www.cell.com/article/S2213671117304782/pdf
dc.identifier.urihttp://hdl.handle.net/10668/11845
dc.issue.number6
dc.journal.titleStem cell reports
dc.journal.titleabbreviationStem Cell Reports
dc.language.isoen
dc.organizationCentro Andaluz de Nanomedicina y Biotecnología-BIONAND
dc.organizationInstituto de Investigación Biomédica de Málaga-IBIMA
dc.page.number1754-1764
dc.pubmedtypeJournal Article
dc.pubmedtypeResearch Support, Non-U.S. Gov't
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectcardiovascular
dc.subjectdifferentiation
dc.subjecthuman pluripotent stem cells
dc.subjectproepicardium
dc.subjectprogenitor cells
dc.subject.meshAnimals
dc.subject.meshBone Morphogenetic Protein 4
dc.subject.meshCardiovascular System
dc.subject.meshCell Adhesion
dc.subject.meshCell Culture Techniques
dc.subject.meshCell Differentiation
dc.subject.meshChick Embryo
dc.subject.meshEmbryonic Development
dc.subject.meshGene Expression Regulation, Developmental
dc.subject.meshHeart
dc.subject.meshHumans
dc.subject.meshInduced Pluripotent Stem Cells
dc.subject.meshMyocardium
dc.subject.meshMyocytes, Cardiac
dc.subject.meshPericardium
dc.subject.meshReceptor, Platelet-Derived Growth Factor alpha
dc.subject.meshStem Cells
dc.subject.meshTretinoin
dc.titleHuman Pluripotent Stem Cell Differentiation into Functional Epicardial Progenitor Cells.
dc.typeresearch article
dc.type.hasVersionVoR
dc.volume.number9
dspace.entity.typePublication

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