Publication:
Pdx1 Is Transcriptionally Regulated by EGR-1 during Nitric Oxide-Induced Endoderm Differentiation of Mouse Embryonic Stem Cells.

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2022-04-01

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Salguero-Aranda, Carmen
Beltran-Povea, Amparo
Postigo-Corrales, Fátima
Hitos, Ana Belén
Díaz, Irene
Caballano-Infantes, Estefanía
Fraga, Mario F
Hmadcha, Abdelkrim
Martín, Franz
Soria, Bernat

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Abstract

The transcription factor, early growth response-1 (EGR-1), is involved in the regulation of cell differentiation, proliferation, and apoptosis in response to different stimuli. EGR-1 is described to be involved in pancreatic endoderm differentiation, but the regulatory mechanisms controlling its action are not fully elucidated. Our previous investigation reported that exposure of mouse embryonic stem cells (mESCs) to the chemical nitric oxide (NO) donor diethylenetriamine nitric oxide adduct (DETA-NO) induces the expression of early differentiation genes such as pancreatic and duodenal homeobox 1 (Pdx1). We have also evidenced that Pdx1 expression is associated with the release of polycomb repressive complex 2 (PRC2) and P300 from the Pdx1 promoter; these events were accompanied by epigenetic changes to histones and site-specific changes in the DNA methylation. Here, we investigate the role of EGR-1 on Pdx1 regulation in mESCs. This study reveals that EGR-1 plays a negative role in Pdx1 expression and shows that the binding capacity of EGR-1 to the Pdx1 promoter depends on the methylation level of its DNA binding site and its acetylation state. These results suggest that targeting EGR-1 at early differentiation stages might be relevant for directing pluripotent cells into Pdx1-dependent cell lineages.

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Animals
Cell Differentiation
Embryonic Stem Cells
Endoderm
Mice
Mouse Embryonic Stem Cells
Nitric Oxide

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Keywords

EGR-1, Pdx1, endoderm differentiation, mESCs, nitric oxide

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