Publication:
Oleic Acid Protects Against Insulin Resistance by Regulating the Genes Related to the PI3K Signaling Pathway

Simple item page
dc.contributor.authorLópez-Gómez, Carlos
dc.contributor.authorSantiago-Fernández, Concepción
dc.contributor.authorGarcía-Serrano, Sara
dc.contributor.authorGarcía-Escobar, Eva
dc.contributor.authorGutiérrez-Repiso, Carolina
dc.contributor.authorRodríguez-Díaz, Cristina
dc.contributor.authorHo-Plágaro, Ailec
dc.contributor.authorMartín-Reyes, Flores
dc.contributor.authorGarrido-Sánchez, Lourdes
dc.contributor.authorValdés, Sergio
dc.contributor.authorRodríguez-Cañete, Alberto
dc.contributor.authorRodríguez-Pacheco, Francisca
dc.contributor.authorGarcía-Fuentes, Eduardo
dc.contributor.authoraffiliation[López-Gómez,C; Santiago-Fernández,C; Rodríguez-Díaz,C; Ho-Plágaro,A; Martín-Reyes,F; Rodríguez-Pacheco,F; García-Fuentes,E] Unidad de Gestión Clínica de Aparato Digestivo, Hospital Universitario Virgen de la Victoria/Instituto de Investigación Biomédica de Málaga-IBIMA, Málaga, Spain. [García-Serrano,S; García-Escobar,E; Valdés,S] Unidad de Gestión Clínica de Endocrinología y Nutrición, Hospital Regional Universitario de Málaga/Instituto de Investigación Biomédica de Málaga-IBIMA, Málaga, Spain. [García-Serrano,S; García-Escobar,E; Valdés,S; Rodríguez-Pacheco,F] CIBER de Diabetes y Enfermedades Metabólicas Asociadas-CIBERDEM, Málaga, Spain. [Gutiérrez-Repiso,C; Garrido-Sánchez,L] Unidad de Gestión Clínica de Endocrinología y Nutrición, Hospital Universitario Virgen de la Victoria/Instituto de Investigación Biomédica de Málaga-IBIMA, Málaga, Spain. [Garrido-Sánchez,L] CIBER Fisiopatología de la Obesidad y Nutrición-CIBEROBN, Málaga, Spain. [Rodríguez-Cañete,A] Unidad de Gestión Clínica de Cirugía General, Digestiva y Trasplantes, Hospital Regional Universitario de Málaga, Málaga, Spain.
dc.contributor.funderThis work was supported in part by a grant from the Instituto de Salud Carlos III, Spain (PI09/01016), and from a grant of the Consejería de Economía, Innovación y Ciencia de la Junta de Andalucía, Spain (CTS8081). This study has been co-funded by FEDER funds (“A way to make Europe”) (“Andalucía se mueve con Europa”). CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN) and CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM) are ISCIII projects. all authors have read the journal’s authorship statement. CLG is supported by a grant from the Ministerio de Educación y Formación Professional (Spain) (IJCI-2017-31466). CSF is supported by a grant from the ISCIII (Spain) (FI16/00241). CGR is supported by a grant from the Ministerio de Educación y Formación Professional (Spain) (IJCI-2017-33065). CRD is supported by a grant from the ISCIII (Spain) (CD18/00188). AHP is supported by a grant from the Ministerio de Educación y Formación Professional (Spain) (FPU14/01972). FMR is supported by a grant from the ISCIII (Spain) (“PFIS” program, FI19/00189). LGS is supported by the Miguel Servet program from the ISCIII (Spain) (“Miguel Servet II” program, CPII18/00030) and Nicolas Monardes program from the Consejería de Salud de Andalucía (Spain) (C-0028-2018). FRP is supported by the Miguel Servet program from the ISCIII (Spain) (“Miguel Servet I” program, CP18/00042). EGF is supported by the Nicolas Monardes program from the Consejería de Salud de Andalucía (Spain) (C-0031-2016).
dc.date.accessioned2022-09-22T08:47:44Z
dc.date.available2022-09-22T08:47:44Z
dc.date.issued2020-08-12
dc.description.abstractThe effects of different types of fatty acids on the gene expression of key players in the IRS1/PI3K signaling pathway have been poorly studied. Material and Methods: We analyzed IRS1, p85α, and p110β mRNA expression and the fatty acid composition of phospholipids in visceral adipose tissue from patients with morbid obesity and from non-obese patients. Moreover, we analyzed the expression of those genes in visceral adipocytes incubated with oleic, linoleic, palmitic and dosahexaenoic acids. Results: We found a reduced IRS1 expression in patients with morbid obesity, independent of insulin resistance, and a reduced p110β expression in those with lower insulin resistance. A positive correlation was found between p85α and stearic acid, and between IRS1 and p110β with palmitic and dosahexaenoic acid. In contrast, a negative correlation was found between p85α and oleic acid, and between IRS1 and p110β with linoleic, arachidonic and adrenic acid. Incubation with palmitic acid decreased IRS1 expression. p85α was down-regulated after incubation with oleic and dosahexaenoic acid and up-regulated with palmitic acid. p110β expression was increased and decreased after incubation with oleic and palmitic acid, respectively. The ratio p85α/p110β was decreased by oleic and dosahexaenoic acid and increased by palmitic acid. Conclusions: Our in vitro results suggest a detrimental role of palmitic acid on the expression of gene related to insulin signaling pathway, with oleic acid being the one with the higher and more beneficial effects. DHA had a slight beneficial effect. Fatty acid-induced regulation of genes related to the IRS1/PI3K pathway may be a novel mechanism by which fatty acids regulate insulin sensitivity in visceral adipocytes.es_ES
dc.description.versionYeses_ES
dc.identifier.citationLópez-Gómez C, Santiago-Fernández C, García-Serrano S, García-Escobar E, Gutiérrez-Repiso C, Rodríguez-Díaz C, et al. Oleic Acid Protects Against Insulin Resistance by Regulating the Genes Related to the PI3K Signaling Pathway. J Clin Med. 2020 Aug 12;9(8):2615es_ES
dc.identifier.doi10.3390/jcm9082615es_ES
dc.identifier.essn2077-0383
dc.identifier.pmcPMC7463472
dc.identifier.pmid32806641es_ES
dc.identifier.urihttp://hdl.handle.net/10668/4103
dc.journal.titleJournal of Clinical Medicine
dc.language.isoen
dc.page.number14 p.
dc.publisherMDPIes_ES
dc.relation.publisherversionhttps://www.mdpi.com/2077-0383/9/8/2615/htmes_ES
dc.rightsAtribución 4.0 Internacional*
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectMorbid obesityes_ES
dc.subjectInsulin resistancees_ES
dc.subjectIRS1es_ES
dc.subjectp85αes_ES
dc.subjectp110βes_ES
dc.subjectPalmitic acides_ES
dc.subjectOleic acides_ES
dc.subjectDocosahexaenoic acides_ES
dc.subjectLinoleic acides_ES
dc.subjectVisceral adipose tissuees_ES
dc.subjectObesidad mórbidaes_ES
dc.subjectResistencia a la insulinaes_ES
dc.subjectÁcido palmíticoes_ES
dc.subjectÁcido oléicoes_ES
dc.subjectÁcidos docosahexaenoicoses_ES
dc.subjectÁcido linoleicoes_ES
dc.subjectGrasa intraabdominales_ES
dc.subjectAndalucíaes_ES
dc.subject.meshMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humanses_ES
dc.subject.meshMedical Subject Headings::Diseases::Nutritional and Metabolic Diseases::Metabolic Diseases::Glucose Metabolism Disorders::Hyperinsulinism::Insulin Resistancees_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Transferases::Phosphotransferases::Phosphotransferases (Alcohol Group Acceptor)::Phosphatidylinositol 3-Kinaseses_ES
dc.subject.meshMedical Subject Headings::Anatomy::Tissues::Connective Tissue::Adipose Tissue::Adipose Tissue, White::Abdominal Fat::Intra-Abdominal Fates_ES
dc.subject.meshMedical Subject Headings::Diseases::Nutritional and Metabolic Diseases::Nutrition Disorders::Overnutrition::Obesity::Obesity, Morbides_ES
dc.subject.meshMedical Subject Headings::Anatomy::Cells::Connective Tissue Cells::Adipocyteses_ES
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Signal Transductiones_ES
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expressiones_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Lipids::Fatty Acids::Palmitic Acidses_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Lipids::Phospholipidses_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Lipids::Fatty Acids::Stearic Acidses_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Lipids::Fatty Acids::Fatty Acids, Unsaturated::Fatty Acids, Monounsaturated::Oleic Acidses_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Hormones, Hormone Substitutes, and Hormone Antagonists::Hormones::Peptide Hormones::Pancreatic Hormones::Insulinses_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::RNA::RNA, Messengeres_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Lipids::Fatty Acids::Fatty Acids, Unsaturatedes_ES
dc.subject.meshMedical Subject Headings::Geographical Locations::Geographic Locations::Europe::Spaines_ES
dc.titleOleic Acid Protects Against Insulin Resistance by Regulating the Genes Related to the PI3K Signaling Pathwayes_ES
dc.typeresearch article
dc.type.hasVersionVoR
dspace.entity.typePublication

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
LopezGomez_OleicAcid.pdf
Size:
505.5 KB
Format:
Adobe Portable Document Format
Description:
Artículo original
Download

Collections

SAS - Hospital Universitario Virgen de la Victoria
Instituto de Investigación Biomédica de Málaga - Plataforma Bionand (IBIMA)
SAS - Hospital Regional Universitario de Málaga

© 2023 – Repositorio Institucional de Salud de Andalucía - Fundación Pública Andaluza Progreso y Salud - Consejería de Salud y Consumo de la Junta de Andalucía