Publication: Prediction of degradation pathways of phenolic compounds in the human gut microbiota through enzyme promiscuity methods.
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Identifiers
Date
2022-06-20
Authors
Balzerani, Francesco
Hinojosa-Nogueira, Daniel
Cendoya, Xabier
Blasco, Telmo
Perez-Burillo, Sergio
Apaolaza, Iñigo
Francino, M Pilar
Rufian-Henares, Jose Angel
Planes, Francisco J
Advisors
Journal Title
Journal ISSN
Volume Title
Publisher
Nature Publishing Group
Abstract
The relevance of phenolic compounds in the human diet has increased in recent years, particularly due to their role as natural antioxidants and chemopreventive agents in different diseases. In the human body, phenolic compounds are mainly metabolized by the gut microbiota; however, their metabolism is not well represented in public databases and existing reconstructions. In a previous work, using different sources of knowledge, bioinformatic and modelling tools, we developed AGREDA, an extended metabolic network more amenable to analyze the interaction of the human gut microbiota with diet. Despite the substantial improvement achieved by AGREDA, it was not sufficient to represent the diverse metabolic space of phenolic compounds. In this article, we make use of an enzyme promiscuity approach to complete further the metabolism of phenolic compounds in the human gut microbiota. In particular, we apply RetroPath RL, a previously developed approach based on Monte Carlo Tree Search strategy reinforcement learning, in order to predict the degradation pathways of compounds present in Phenol-Explorer, the largest database of phenolic compounds in the literature. Reactions predicted by RetroPath RL were integrated with AGREDA, leading to a more complete version of the human gut microbiota metabolic network. We assess the impact of our improvements in the metabolic processing of various foods, finding previously undetected connections with output microbial metabolites. By means of untargeted metabolomics data, we present in vitro experimental validation for output microbial metabolites released in the fermentation of lentils with feces of children representing different clinical conditions.
Description
MeSH Terms
Child
Feces
Fermentation
Gastrointestinal Microbiome
Humans
Metabolomics
Phenols
Feces
Fermentation
Gastrointestinal Microbiome
Humans
Metabolomics
Phenols
DeCS Terms
Fenoles
Fermentación
Heces
Humanos
Metabolómica
Microbioma gastrointestinal
Niño
Fermentación
Heces
Humanos
Metabolómica
Microbioma gastrointestinal
Niño
CIE Terms
Keywords
Physiology, Biochemical networks, Computational biology and bioinformatics
Citation
Balzerani F, Hinojosa-Nogueira D, Cendoya X, Blasco T, Pérez-Burillo S, Apaolaza I, et al. Prediction of degradation pathways of phenolic compounds in the human gut microbiota through enzyme promiscuity methods. NPJ Syst Biol Appl. 2022 Jul 12;8(1):24.