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Biological Effects of Maslinic Acid on Human Epithelial Cells Used in Tissue Engineering.

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dc.contributor.authorOrtiz-Arrabal, Olimpia
dc.contributor.authorChato-Astrain, Jesus
dc.contributor.authorCrespo, Pascual Vicente
dc.contributor.authorGarzon, Ingrid
dc.contributor.authorMesa-Garcia, Maria Dolores
dc.contributor.authorAlaminos, Miguel
dc.contributor.authorGomez-Llorente, Carolina
dc.contributor.funderConsejería de Salud y Familias, Junta de Andalucía, Spain,
dc.contributor.funderproyectos de I + D + i en el marco del Programa Operativo FEDER Andalucía 2014-2020, Universidad de Granada and Consejería de Transformación Económica, Industria, Conocimiento y Universidades)
dc.contributor.funderSpanish Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica (I + D + i) of the Spanish Ministry of Science and Innovation
dc.contributor.funderInstituto de Salud Carlos III, co-financed by the European Regional Development Fund (ERDF) through the “Una manera de hacer Europa” program, European Union.
dc.date.accessioned2023-05-03T13:38:55Z
dc.date.available2023-05-03T13:38:55Z
dc.date.issued2022-04-08
dc.description.abstractIn the present work, we evaluated the potential of maslinic acid (MA) to improve currently available keratinocyte culture methods for use in skin tissue engineering. Results showed that MA can increase cell proliferation and WST-1 activity of human keratinocytes after 24, 48, and 72 h, especially at the concentration of 5 μg/ml, without affecting cell viability. This effect was associated to a significant increase of KI-67 protein expression and upregulation of several genes associated to cell proliferation (PCNA) and differentiation (cytokeratins, intercellular junctions and basement membrane related genes). When human keratinocytes were isolated from skin biopsies, we found that MA at the concentration of 5 μg/ml significantly increased the efficiency of the explant and the cell dissociation methods. These results revealed the positive effects of MA to optimize human keratinocyte culture protocols for use in skin tissue engineering.
dc.description.versionSi
dc.identifier.citationOrtiz-Arrabal O, Chato-Astrain J, Crespo PV, Garzón I, Mesa-García MD, Alaminos M, et al. Biological Effects of Maslinic Acid on Human Epithelial Cells Used in Tissue Engineering. Front Bioeng Biotechnol. 2022 Apr 27;10:876734.
dc.identifier.doi10.3389/fbioe.2022.876734
dc.identifier.issn2296-4185
dc.identifier.pmcPMC9159156
dc.identifier.pmid35662841
dc.identifier.pubmedURLhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9159156/pdf
dc.identifier.unpaywallURLhttps://www.frontiersin.org/articles/10.3389/fbioe.2022.876734/pdf
dc.identifier.urihttp://hdl.handle.net/10668/20523
dc.journal.titleFrontiers in bioengineering and biotechnology
dc.journal.titleabbreviationFront Bioeng Biotechnol
dc.language.isoen
dc.organizationInstituto de Investigación Biosanitaria de Granada (ibs.GRANADA)
dc.page.number11
dc.provenanceRealizada la curación de contenido 13/08/2024
dc.publisherFrontiers Research Foundation
dc.pubmedtypeJournal Article
dc.relation.projectIDB-CTS-450-UGR20
dc.relation.projectIDPE-0395-2019
dc.relation.projectIDICI19-00024 (BIOCLEFT)
dc.relation.projectIDFIS PI20/0317
dc.relation.projectIDFIS PI21/0980
dc.relation.publisherversionhttps://doi.org/10.3389/fbioe.2022.876734
dc.rightsAttribution 4.0 International
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectcell culture
dc.subjectcell proliferation
dc.subjectepithelial cells
dc.subjectmaslinic acid
dc.subjecttissue engineering
dc.subject.decsAntígeno Ki-67
dc.subject.decsAntígeno nuclear de célula en proliferación
dc.subject.decsHumanos
dc.subject.decsIngeniería de tejidos
dc.subject.decsMembrana basal
dc.subject.decsProliferación celular
dc.subject.decsQueratinas
dc.subject.decsQueratinocitos
dc.subject.decsRegulación hacia arriba
dc.subject.decsSupervivencia celular
dc.subject.decsÁcidos
dc.subject.meshHumans
dc.subject.meshHumans
dc.subject.meshKi-67 Antigen
dc.subject.meshProliferating Cell Nuclear Antigen
dc.subject.meshTissue Engineering
dc.subject.meshBasement Membrane
dc.subject.meshCell Survival
dc.subject.meshUp-Regulation
dc.subject.meshmaslinic acid
dc.subject.meshKeratinocytes
dc.subject.meshKeratins
dc.subject.meshCell Proliferation
dc.titleBiological Effects of Maslinic Acid on Human Epithelial Cells Used in Tissue Engineering.
dc.typeresearch article
dc.type.hasVersionVoR
dc.volume.number10
dspace.entity.typePublication

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