Publication: Insulin and Leptin Signaling in Placenta from Gestational Diabetic Subjects.
dc.contributor.author | Pérez-Pérez, A. | |
dc.contributor.author | Guadix, P. | |
dc.contributor.author | Maymó, J. | |
dc.contributor.author | Dueñas, J. L. | |
dc.contributor.author | Varone, C. | |
dc.contributor.author | Fernández-Sánchez, M. | |
dc.contributor.author | Sánchez-Margalet, V. | |
dc.contributor.authoraffiliation | [Pérez-Pérez,A; Sánchez-Margalet,V] Department of Medical Biochemistry and Molecular Biology, School of Medicine, Virgen Macarena University Hospital, Seville, Spain. [Guadix,P; Dueñas,JL] Obstetrics and Gynecology Service, Virgen Macarena University Hospital, Seville, Spain. [Maymó,J; Varone,C] Department of Biological Chemistry, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina. [Fernández-Sánchez,M] IVI Seville, Seville, Spain. | es |
dc.date.accessioned | 2016-06-08T08:39:11Z | |
dc.date.available | 2016-06-08T08:39:11Z | |
dc.date.issued | 2016-01 | |
dc.description | Journal Article; | es |
dc.description.abstract | Insulin and leptin receptors are known to share signaling pathways, such as JAK2/STAT-3 (Janus kinase2/signal transduction and activator of transcription3), MAPK (Mitogen activated protein kinase), and PI3K (phosphoinositide 3-kinase). Both positive and negative cross-talk have been previously found in different cellular systems. Gestational diabetes (GDM) is a pathophysiological state with high circulating levels of both insulin and leptin. We have previously found that these 3 signaling pathways are activated in placenta from GDM patients to promote translation, involving the activation of leptin receptor. Now, we have tested the hypothesis that both leptin and insulin receptors might contribute to this activation in a positive way that may become negative when the system is overactivated. We studied the activation of leptin and insulin receptors in placenta from GDM and healthy pregnancies. We have also performed in vitro studies with insulin and leptin stimulation of trophoblast explants from healthy placenta. We have found that both leptin and insulin receptors are activated in placenta from GDM. In vitro stimulation of trophoblast explants with both leptin and insulin at submaximal doses (0.1 nM) potentiated the activation of signaling, whereas preincubation with maximal concentrations of insulin (10 nM) and further stimulation with leptin showed negative effect. Trophoblastic explants from GDM placenta, which presented high signaling levels, had a negative signaling effect when further incubated in vitro with leptin. In conclusion, insulin and leptin receptors have positive effects on signaling, contributing to high signaling levels in GDM placenta, but insulin and leptin have negative effects upon overstimulation. | es |
dc.description.version | Yes | es |
dc.identifier.citation | Pérez-Pérez A, Guadix P, Maymó J, Dueñas JL, Varone C, Fernández-Sánchez M, et al. Insulin and Leptin Signaling in Placenta from Gestational Diabetic Subjects. Horm. Metab. Res. 2016; 48(1):62-9 | es |
dc.identifier.doi | 10.1055/s-0035-1559722 | |
dc.identifier.essn | 1439-4286 | |
dc.identifier.issn | 0018-5043 | |
dc.identifier.pmid | 26584065 | |
dc.identifier.uri | http://hdl.handle.net/10668/2210 | |
dc.journal.title | Hormone and Metabolic Research | |
dc.language.iso | en | |
dc.publisher | Thieme Publishing | es |
dc.relation.publisherversion | https://www.thieme-connect.com/DOI/DOI?10.1055/s-0035-1559722 | es |
dc.rights.accessRights | open access | |
dc.subject | Leptin | es |
dc.subject | Insulin | es |
dc.subject | Signal transduction | es |
dc.subject | Gestational diabetes | es |
dc.subject | Placenta | es |
dc.subject | Proteínas quinasas activadas por mitógenos | es |
dc.subject | Fosfatidilinositol 3-Quinasas | es |
dc.subject | Trofoblastos | es |
dc.subject.mesh | Medical Subject Headings::Diseases::Female Urogenital Diseases and Pregnancy Complications::Pregnancy Complications::Diabetes, Gestational | es |
dc.subject.mesh | Medical Subject Headings::Check Tags::Female | es |
dc.subject.mesh | Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans | es |
dc.subject.mesh | Medical Subject Headings::Chemicals and Drugs::Hormones, Hormone Substitutes, and Hormone Antagonists::Hormones::Peptide Hormones::Pancreatic Hormones::Insulins | es |
dc.subject.mesh | Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Transferases::Phosphotransferases::Phosphotransferases (Alcohol Group Acceptor)::Protein Kinases::Protein-Tyrosine Kinases::Janus Kinases::Janus Kinase 2 | es |
dc.subject.mesh | Medical Subject Headings::Chemicals and Drugs::Hormones, Hormone Substitutes, and Hormone Antagonists::Hormones::Peptide Hormones::Adipokines::Leptin | es |
dc.subject.mesh | Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Transferases::Phosphotransferases::Phosphotransferases (Alcohol Group Acceptor)::Protein Kinases::Protein-Serine-Threonine Kinases::Mitogen-Activated Protein Kinases | es |
dc.subject.mesh | Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Transferases::Phosphotransferases::Phosphotransferases (Alcohol Group Acceptor)::Phosphatidylinositol 3-Kinases::Phosphatidylinositol 3-Kinase | es |
dc.subject.mesh | Medical Subject Headings::Phenomena and Processes::Reproductive and Urinary Physiological Phenomena::Reproductive Physiological Phenomena::Reproductive Physiological Processes::Reproduction::Pregnancy | es |
dc.subject.mesh | Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Transferases::Phosphotransferases::Phosphotransferases (Alcohol Group Acceptor)::Protein Kinases::Protein-Tyrosine Kinases::Receptor Protein-Tyrosine Kinases::Receptor, Insulin | es |
dc.subject.mesh | Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Membrane Proteins::Receptors, Cell Surface::Receptors, Adipokine::Receptors, Leptin | es |
dc.subject.mesh | Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::Signal Transduction | es |
dc.subject.mesh | Medical Subject Headings::Anatomy::Cells::Endocrine Cells::Trophoblasts | es |
dc.title | Insulin and Leptin Signaling in Placenta from Gestational Diabetic Subjects. | es |
dc.type | research article | |
dc.type.hasVersion | VoR | |
dspace.entity.type | Publication |
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