Publication:
Probiotics Prevent Hypertension in a Murine Model of Systemic Lupus Erythematosus Induced by Toll-Like Receptor 7 Activation.

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Date

2021-07-30

Authors

de la Visitacion, Nestor
Robles-Vera, Iñaki
Moleon-Moya, Javier
Sanchez, Manuel
Jimenez, Rosario
Gomez-Guzman, Manuel
Gonzalez-Correa, Cristina
Olivares, Monica
Toral, Marta
Romero, Miguel

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MDPI AG
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Abstract

Our group tested the effects of Lactobacillus fermentum CECT5716 (LC40) and/or Bifidobacterium breve CECT7263 (BFM) in the prevention of gut dysbiosis, hypertension and endothelial dysfunction in a pharmacologically-induced model of systemic lupus erythematosus (SLE). We treated eight-week-old BALB/cByJRj mice without (Ctrl) or with the agonist of TLR-7 Imiquimod (IMQ) for 8 weeks. Concomitantly, LC40 (109 CFU/mL) and BFM (109 CFU/mL) were administered through oral gavage once a day. IMQ induced intestinal dysbiosis consisting of a decrease in the α-diversity measured with Chao-richness and numbers of species. LC40 and BFM did not restore these parameters. The three-dimensional principal component analysis of bacterial taxa in stool samples presented perfect clustering between Ctrl and IMQ groups. Clusters corresponding to LC40 and BFM were more akin to IMQ. BFM and LC40 were detected colonizing the gut microbiota of mice treated respectively. LC40 and BFM decreased plasma double-stranded DNA autoantibodies, and B cells in spleen, which were increased in the IMQ group. Also, LC40 and BFM treatments activated TLR9, reduced T cells activation, and Th17 polarization in mesenteric lymph nodes. Aortae from IMQ mice displayed a decreased endothelium-dependent vasodilator response to acetylcholine linked to pro-inflammatory and pro-oxidative status, which were normalized by both BFM and LC40. In conclusion, we demonstrate for the first time that the chronic treatment with LC40 or BFM prevented hypertension and endothelial dysfunction in a mouse lupus model induced by TLR-7 activation.

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MeSH Terms

Animals
Bifidobacterium breve
Blood Pressure
Disease Models, Animal
Dysbiosis
Female
Gastrointestinal Microbiome
Hypertension
Immunity
Limosilactobacillus fermentum
Lupus Erythematosus, Systemic
Membrane Glycoproteins
Mice
Mice, Inbred BALB C
NADPH Oxidases
Oxidative Stress
Probiotics
RNA, Ribosomal, 16S
Reactive Oxygen Species
Toll-Like Receptor 7

DeCS Terms

ARN ribosómico 16S
Bifidobacterium breve
Disbiosis
Especies reactivas de oxígeno
Estrés oxidativo
Glicoproteínas de membrana
Hipertensión
Inmunidad
Limosilactobacillus fermentum
Lupus eritematoso sistémico
NADPH oxidasas
Presión sanguínea

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Keywords

TLR-7 activation, endothelial dysfunction, hypertension, lupus, probiotics

Citation

de la Visitación N, Robles-Vera I, Moleón-Moya J, Sánchez M, Jiménez R, Gómez-Guzmán M, et al. Probiotics Prevent Hypertension in a Murine Model of Systemic Lupus Erythematosus Induced by Toll-Like Receptor 7 Activation. Nutrients. 2021 Jul 31;13(8):2669.