Publication: Chromatin modifiers and recombination factors promote a telomere fold-back structure, that is lost during replicative senescence
dc.contributor.author | Wagner, Tina | |
dc.contributor.author | Pérez-Martínez, Lara | |
dc.contributor.author | Schellhaas, René | |
dc.contributor.author | Barrientos-Moreno, Marta | |
dc.contributor.author | Öztürk, Merve | |
dc.contributor.author | Prado, Félix | |
dc.contributor.author | Butter, Falk | |
dc.contributor.author | Luke, Brian | |
dc.contributor.authoraffiliation | [Wagner,T; Luke,B] Institute of Developmental Biology and Neurobiology (IDN), Johannes Gutenberg-Universität, Mainz, Germany. [Pérez-Martínez,L; Schellhaas,R; Öztürk,M; Butter,F; Luke,B] Institute of Molecular Biology (IMB) gGmbH, Mainz, Germany. [Barrientos-Moreno,M; Prado,F] Department of Genome Biology, Andalusian Molecular Biology and Regenerative Medicine Center (CABIMER), CSIC-University of Seville-University Pablo de Olavide, Seville, Spain. | |
dc.contributor.funder | BL’s lab was supported by the Heisenberg Program of the Deutsche Forschungsgemeinschaft DFG - LU 1709/2-1. Research in FP’s lab was funded by grants from the Spanish government, Ministerio de Economía y Competitividad (BFU2015-63689-P). | |
dc.date.accessioned | 2022-07-11T08:15:32Z | |
dc.date.available | 2022-07-11T08:15:32Z | |
dc.date.issued | 2020-12-28 | |
dc.description.abstract | Telomeres have the ability to adopt a lariat conformation and hence, engage in long and short distance intra-chromosome interactions. Budding yeast telomeres were proposed to fold back into subtelomeric regions, but a robust assay to quantitatively characterize this structure has been lacking. Therefore, it is not well understood how the interactions between telomeres and non-telomeric regions are established and regulated. We employ a telomere chromosome conformation capture (Telo-3C) approach to directly analyze telomere folding and its maintenance in S. cerevisiae. We identify the histone modifiers Sir2, Sin3 and Set2 as critical regulators for telomere folding, which suggests that a distinct telomeric chromatin environment is a major requirement for the folding of yeast telomeres. We demonstrate that telomeres are not folded when cells enter replicative senescence, which occurs independently of short telomere length. Indeed, Sir2, Sin3 and Set2 protein levels are decreased during senescence and their absence may thereby prevent telomere folding. Additionally, we show that the homologous recombination machinery, including the Rad51 and Rad52 proteins, as well as the checkpoint component Rad53 are essential for establishing the telomere fold-back structure. This study outlines a method to interrogate telomere-subtelomere interactions at a single unmodified yeast telomere. Using this method, we provide insights into how the spatial arrangement of the chromosome end structure is established and demonstrate that telomere folding is compromised throughout replicative senescence. | es_ES |
dc.description.version | Yes | es_ES |
dc.identifier.citation | Wagner T, Pérez-Martínez L, Schellhaas R, Barrientos-Moreno M, Öztürk M, Prado F, et al. Chromatin modifiers and recombination factors promote a telomere fold-back structure, that is lost during replicative senescence. PLoS Genet. 2020 Dec 28;16(12):e1008603 | es_ES |
dc.identifier.doi | 10.1371/journal.pgen.1008603 | es_ES |
dc.identifier.essn | 1553-7404 | |
dc.identifier.pmc | PMC7793543 | |
dc.identifier.pmid | 33370275 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10668/3774 | |
dc.journal.title | PLOS Genetics | |
dc.language.iso | en | |
dc.page.number | 28 p. | |
dc.publisher | Public Library of Science | es_ES |
dc.relation.publisherversion | https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1008603 | es_ES |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.accessRights | Acceso abierto | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Chromatin | es_ES |
dc.subject | Telomere | es_ES |
dc.subject | Chromosomes | es_ES |
dc.subject | Saccharomyces cerevisiae | es_ES |
dc.subject | DNA Damage | es_ES |
dc.subject | Cellular Senescence | es_ES |
dc.subject | Rad52 DNA Repair and Recombination Protein | es_ES |
dc.subject | Cromatina | es_ES |
dc.subject | Telómero | es_ES |
dc.subject | Cromosomas | es_ES |
dc.subject | Daño del ADN | es_ES |
dc.subject | Senescencia celular | es_ES |
dc.subject | Proteína recombinante y reparadora de ADN Rad52 | es_ES |
dc.subject.mesh | Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases::Amidohydrolases::Histone Deacetylases | es_ES |
dc.subject.mesh | Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Transferases::One-Carbon Group Transferases::Methyltransferases | es_ES |
dc.subject.mesh | Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Recombinases::Rec A Recombinases::Rad51 Recombinase | es_ES |
dc.subject.mesh | Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::DNA-Binding Proteins::Rad52 DNA Repair and Recombination Protein | es_ES |
dc.subject.mesh | Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Transcription Factors::Repressor Proteins | es_ES |
dc.subject.mesh | Medical Subject Headings::Organisms::Eukaryota::Fungi::Yeasts::Saccharomyces::Saccharomyces cerevisiae | es_ES |
dc.subject.mesh | Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Fungal Proteins::Saccharomyces cerevisiae Proteins | es_ES |
dc.subject.mesh | Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Fungal Proteins::Saccharomyces cerevisiae Proteins::Silent Information Regulator Proteins, Saccharomyces cerevisiae | es_ES |
dc.subject.mesh | Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Intracellular Signaling Peptides and Proteins::Sirtuins::Sirtuin 2 | es_ES |
dc.subject.mesh | Medical Subject Headings::Anatomy::Cells::Cellular Structures::Intracellular Space::Cell Nucleus::Cell Nucleus Structures::Intranuclear Space::Chromosomes::Chromosome Structures::Telomere | es_ES |
dc.subject.mesh | Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Cycle::Cell Division::Telomere Homeostasis | es_ES |
dc.subject.mesh | Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::DNA Replication | es_ES |
dc.title | Chromatin modifiers and recombination factors promote a telomere fold-back structure, that is lost during replicative senescence | es_ES |
dc.type | research article | |
dc.type.hasVersion | VoR | |
dspace.entity.type | Publication |
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