Publication:
Chromatin modifiers and recombination factors promote a telomere fold-back structure, that is lost during replicative senescence

dc.contributor.authorWagner, Tina
dc.contributor.authorPérez-Martínez, Lara
dc.contributor.authorSchellhaas, René
dc.contributor.authorBarrientos-Moreno, Marta
dc.contributor.authorÖztürk, Merve
dc.contributor.authorPrado, Félix
dc.contributor.authorButter, Falk
dc.contributor.authorLuke, Brian
dc.contributor.authoraffiliation[Wagner,T; Luke,B] Institute of Developmental Biology and Neurobiology (IDN), Johannes Gutenberg-Universität, Mainz, Germany. [Pérez-Martínez,L; Schellhaas,R; Öztürk,M; Butter,F; Luke,B] Institute of Molecular Biology (IMB) gGmbH, Mainz, Germany. [Barrientos-Moreno,M; Prado,F] Department of Genome Biology, Andalusian Molecular Biology and Regenerative Medicine Center (CABIMER), CSIC-University of Seville-University Pablo de Olavide, Seville, Spain.
dc.contributor.funderBL’s lab was supported by the Heisenberg Program of the Deutsche Forschungsgemeinschaft DFG - LU 1709/2-1. Research in FP’s lab was funded by grants from the Spanish government, Ministerio de Economía y Competitividad (BFU2015-63689-P).
dc.date.accessioned2022-07-11T08:15:32Z
dc.date.available2022-07-11T08:15:32Z
dc.date.issued2020-12-28
dc.description.abstractTelomeres have the ability to adopt a lariat conformation and hence, engage in long and short distance intra-chromosome interactions. Budding yeast telomeres were proposed to fold back into subtelomeric regions, but a robust assay to quantitatively characterize this structure has been lacking. Therefore, it is not well understood how the interactions between telomeres and non-telomeric regions are established and regulated. We employ a telomere chromosome conformation capture (Telo-3C) approach to directly analyze telomere folding and its maintenance in S. cerevisiae. We identify the histone modifiers Sir2, Sin3 and Set2 as critical regulators for telomere folding, which suggests that a distinct telomeric chromatin environment is a major requirement for the folding of yeast telomeres. We demonstrate that telomeres are not folded when cells enter replicative senescence, which occurs independently of short telomere length. Indeed, Sir2, Sin3 and Set2 protein levels are decreased during senescence and their absence may thereby prevent telomere folding. Additionally, we show that the homologous recombination machinery, including the Rad51 and Rad52 proteins, as well as the checkpoint component Rad53 are essential for establishing the telomere fold-back structure. This study outlines a method to interrogate telomere-subtelomere interactions at a single unmodified yeast telomere. Using this method, we provide insights into how the spatial arrangement of the chromosome end structure is established and demonstrate that telomere folding is compromised throughout replicative senescence.es_ES
dc.description.versionYeses_ES
dc.identifier.citationWagner T, Pérez-Martínez L, Schellhaas R, Barrientos-Moreno M, Öztürk M, Prado F, et al. Chromatin modifiers and recombination factors promote a telomere fold-back structure, that is lost during replicative senescence. PLoS Genet. 2020 Dec 28;16(12):e1008603es_ES
dc.identifier.doi10.1371/journal.pgen.1008603es_ES
dc.identifier.essn1553-7404
dc.identifier.pmcPMC7793543
dc.identifier.pmid33370275es_ES
dc.identifier.urihttp://hdl.handle.net/10668/3774
dc.journal.titlePLOS Genetics
dc.language.isoen
dc.page.number28 p.
dc.publisherPublic Library of Sciencees_ES
dc.relation.publisherversionhttps://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1008603es_ES
dc.rightsAtribución 4.0 Internacional*
dc.rights.accessRightsAcceso abiertoes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectChromatines_ES
dc.subjectTelomerees_ES
dc.subjectChromosomeses_ES
dc.subjectSaccharomyces cerevisiaees_ES
dc.subjectDNA Damagees_ES
dc.subjectCellular Senescencees_ES
dc.subjectRad52 DNA Repair and Recombination Proteines_ES
dc.subjectCromatinaes_ES
dc.subjectTelómeroes_ES
dc.subjectCromosomases_ES
dc.subjectDaño del ADNes_ES
dc.subjectSenescencia celulares_ES
dc.subjectProteína recombinante y reparadora de ADN Rad52es_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases::Amidohydrolases::Histone Deacetylaseses_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Transferases::One-Carbon Group Transferases::Methyltransferaseses_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Recombinases::Rec A Recombinases::Rad51 Recombinasees_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::DNA-Binding Proteins::Rad52 DNA Repair and Recombination Proteines_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Transcription Factors::Repressor Proteinses_ES
dc.subject.meshMedical Subject Headings::Organisms::Eukaryota::Fungi::Yeasts::Saccharomyces::Saccharomyces cerevisiaees_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Fungal Proteins::Saccharomyces cerevisiae Proteinses_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Fungal Proteins::Saccharomyces cerevisiae Proteins::Silent Information Regulator Proteins, Saccharomyces cerevisiaees_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Intracellular Signaling Peptides and Proteins::Sirtuins::Sirtuin 2es_ES
dc.subject.meshMedical Subject Headings::Anatomy::Cells::Cellular Structures::Intracellular Space::Cell Nucleus::Cell Nucleus Structures::Intranuclear Space::Chromosomes::Chromosome Structures::Telomerees_ES
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Cycle::Cell Division::Telomere Homeostasises_ES
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::DNA Replicationes_ES
dc.titleChromatin modifiers and recombination factors promote a telomere fold-back structure, that is lost during replicative senescencees_ES
dc.typeresearch article
dc.type.hasVersionVoR
dspace.entity.typePublication

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