Publication: DNA degradation in human teeth exposed to thermal stress
dc.contributor.author | Lozano-Peral, Diego | |
dc.contributor.author | Rubio, Leticia | |
dc.contributor.author | Santos, Ignacio | |
dc.contributor.author | Gaitán, María Jesús | |
dc.contributor.author | Viguera, Enrique | |
dc.contributor.author | Martín-de-Las-Heras, Stella | |
dc.contributor.authoraffiliation | [Lozano-Peral,D; Rubio,L; Santos,I; Gaitán,MJ; Martín-de-Las-Heras,S] Department of Forensic Dentistry and Medicine, Instituto de Investigación Biomédica de Málaga-IBIMA (CE-18), School of Medicine, University of Malaga, Malaga, Spain. [Lozano-Peral.D] Supercomputing and Bioinnovation Center, Servicios Centrales de Apoyo a la Investigación, University of Malaga, Malaga, Spain. [Viguera,E] Department of Cellular Biology, Genetics and Physiology, University of Malaga, Malaga, Spain. [Lozano-Peral,D; Rubio,L] These authors contributed equally: Diego Lozano-Peral and Leticia Rubio | |
dc.contributor.funder | Tis research was funded by the University of Malaga (PPIT.UMA.B1.2020/20). | |
dc.date.accessioned | 2022-11-03T10:24:44Z | |
dc.date.available | 2022-11-03T10:24:44Z | |
dc.date.issued | 2021-06-09 | |
dc.description.abstract | Human identification from burned remains poses a challenge to forensic laboratories, and DNA profiling is widely used for this purpose. Our aim was to evaluate the effect of temperature on DNA degradation in human teeth. Thirty teeth were exposed to temperatures of 100, 200, or 400 °C for 60 min. DNA was quantified by Real-Time qPCR (Quantifiler Human DNA Quantification Kit) and fluorescence spectroscopy (Qubit 3.0 Fluorometer). DNA degradation was evaluated by using STR markers (AmpFLSTR Identifiler Plus PCR Amplification Kit) to determine the allele and locus dropout, inter-locus balance, and degradation slope (observed (Oa) to expected (Ea) locus peak height ratio against the molecular weight). Most of the genomic DNA was degraded between 100 °C and 200 °C. At 100 °C, locus dropout ratios showed significant differences between the largest loci (FGA, D7S820, D18S51, D16S539, D2S1338 and CSF1PO) and amelogenin. Inter-locus balance values significantly differed between all dye channels except between NED and PET. The dropout ratio between D18S51 (NED) and amelogenin (PET) can be recommended for the evaluation of DNA degradation. The Oa/Ea regression model can predict locus peak heights in DNA degradation (R2 = 0.7881). These findings may be useful to assess the reliability of DNA typing for human identification in teeth subjected to prolonged incineration. | es_ES |
dc.description.version | Yes | es_ES |
dc.identifier.citation | Lozano-Peral D, Rubio L, Santos I, Gaitán MJ, Viguera E, Martín-de-Las-Heras S. DNA degradation in human teeth exposed to thermal stress. Sci Rep. 2021 Jun 9;11(1):12118 | es_ES |
dc.identifier.doi | 10.1038/s41598-021-91505-8 | es_ES |
dc.identifier.essn | 2045-2322 | |
dc.identifier.pmc | PMC8190102 | |
dc.identifier.pmid | 34108558 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10668/4322 | |
dc.journal.title | Scientific Reports | |
dc.language.iso | en | |
dc.page.number | 9 p. | |
dc.publisher | Nature Publishing Group | es_ES |
dc.relation.publisherversion | https://www.nature.com/articles/s41598-021-91505-8 | es_ES |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.accessRights | Acceso abierto | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Amelogenin | es_ES |
dc.subject | Incineration | es_ES |
dc.subject | Molecular weight | es_ES |
dc.subject | DNA degradation | es_ES |
dc.subject | Teeth | es_ES |
dc.subject | Amelogenina | es_ES |
dc.subject | Odontología forense | es_ES |
dc.subject | Incineración | es_ES |
dc.subject | Peso molecular | es_ES |
dc.subject | Fragmentación del ADN | es_ES |
dc.subject | Diente | es_ES |
dc.subject.mesh | Medical Subject Headings::Persons::Persons::Age Groups::Adult | es_ES |
dc.subject.mesh | Medical Subject Headings::Persons::Persons::Age Groups::Adult::Aged | es_ES |
dc.subject.mesh | Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Biometry::Biometric Identification::DNA Fingerprinting | es_ES |
dc.subject.mesh | Medical Subject Headings::Check Tags::Female | es_ES |
dc.subject.mesh | Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans | es_ES |
dc.subject.mesh | Medical Subject Headings::Check Tags::Male | es_ES |
dc.subject.mesh | Medical Subject Headings::Persons::Persons::Age Groups::Adult::Middle Aged | es_ES |
dc.subject.mesh | Medical Subject Headings::Anatomy::Stomatognathic System::Mouth::Dentition::Tooth | es_ES |
dc.subject.mesh | Medical Subject Headings::Persons::Persons::Age Groups::Adult::Young Adult | es_ES |
dc.subject.mesh | Medical Subject Headings::Phenomena and Processes::Physiological Phenomena::Physiological Processes::Stress, Physiological::Heat-Shock Response | es_ES |
dc.subject.mesh | Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Dental Enamel Proteins::Amelogenin | es_ES |
dc.subject.mesh | Medical Subject Headings::Phenomena and Processes::Biological Phenomena::Ecological and Environmental Phenomena::Environment::Atmosphere::Weather::Temperature | es_ES |
dc.subject.mesh | Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Structures::Genome::Genome Components::Genes::Alleles | es_ES |
dc.subject.mesh | Medical Subject Headings::Anthropology, Education, Sociology and Social Phenomena::Social Sciences::Anthropology::Anthropology, Physical::Forensic Anthropology | es_ES |
dc.subject.mesh | Medical Subject Headings::Health Care::Environment and Public Health::Public Health::Sanitation::Sanitary Engineering::Waste Management::Refuse Disposal::Incineration | es_ES |
dc.subject.mesh | Medical Subject Headings::Health Care::Health Care Facilities, Manpower, and Services::Health Facilities::Laboratories | es_ES |
dc.subject.mesh | Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Physicochemical Phenomena::Molecular Weight | es_ES |
dc.subject.mesh | Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Research Design::Reproducibility of Results | es_ES |
dc.subject.mesh | Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Photometry::Luminescent Measurements::Fluorometry::Spectrometry, Fluorescence | es_ES |
dc.subject.mesh | Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction | es_ES |
dc.subject.mesh | Medical Subject Headings::Disciplines and Occupations::Natural Science Disciplines::Biological Science Disciplines::Biology::Computational Biology::Genomics | es_ES |
dc.subject.mesh | Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Imaging::Image Interpretation, Computer-Assisted::Tomography, Emission-Computed::Positron-Emission Tomography | es_ES |
dc.title | DNA degradation in human teeth exposed to thermal stress | es_ES |
dc.type | research article | |
dc.type.hasVersion | VoR | |
dspace.entity.type | Publication |
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