Publication:
DNA degradation in human teeth exposed to thermal stress

dc.contributor.authorLozano-Peral, Diego
dc.contributor.authorRubio, Leticia
dc.contributor.authorSantos, Ignacio
dc.contributor.authorGaitán, María Jesús
dc.contributor.authorViguera, Enrique
dc.contributor.authorMartín-de-Las-Heras, Stella
dc.contributor.authoraffiliation[Lozano-Peral,D; Rubio,L; Santos,I; Gaitán,MJ; Martín-de-Las-Heras,S] Department of Forensic Dentistry and Medicine, Instituto de Investigación Biomédica de Málaga-IBIMA (CE-18), School of Medicine, University of Malaga, Malaga, Spain. [Lozano-Peral.D] Supercomputing and Bioinnovation Center, Servicios Centrales de Apoyo a la Investigación, University of Malaga, Malaga, Spain. [Viguera,E] Department of Cellular Biology, Genetics and Physiology, University of Malaga, Malaga, Spain. [Lozano-Peral,D; Rubio,L] These authors contributed equally: Diego Lozano-Peral and Leticia Rubio
dc.contributor.funderTis research was funded by the University of Malaga (PPIT.UMA.B1.2020/20).
dc.date.accessioned2022-11-03T10:24:44Z
dc.date.available2022-11-03T10:24:44Z
dc.date.issued2021-06-09
dc.description.abstractHuman identification from burned remains poses a challenge to forensic laboratories, and DNA profiling is widely used for this purpose. Our aim was to evaluate the effect of temperature on DNA degradation in human teeth. Thirty teeth were exposed to temperatures of 100, 200, or 400 °C for 60 min. DNA was quantified by Real-Time qPCR (Quantifiler Human DNA Quantification Kit) and fluorescence spectroscopy (Qubit 3.0 Fluorometer). DNA degradation was evaluated by using STR markers (AmpFLSTR Identifiler Plus PCR Amplification Kit) to determine the allele and locus dropout, inter-locus balance, and degradation slope (observed (Oa) to expected (Ea) locus peak height ratio against the molecular weight). Most of the genomic DNA was degraded between 100 °C and 200 °C. At 100 °C, locus dropout ratios showed significant differences between the largest loci (FGA, D7S820, D18S51, D16S539, D2S1338 and CSF1PO) and amelogenin. Inter-locus balance values significantly differed between all dye channels except between NED and PET. The dropout ratio between D18S51 (NED) and amelogenin (PET) can be recommended for the evaluation of DNA degradation. The Oa/Ea regression model can predict locus peak heights in DNA degradation (R2 = 0.7881). These findings may be useful to assess the reliability of DNA typing for human identification in teeth subjected to prolonged incineration.es_ES
dc.description.versionYeses_ES
dc.identifier.citationLozano-Peral D, Rubio L, Santos I, Gaitán MJ, Viguera E, Martín-de-Las-Heras S. DNA degradation in human teeth exposed to thermal stress. Sci Rep. 2021 Jun 9;11(1):12118es_ES
dc.identifier.doi10.1038/s41598-021-91505-8es_ES
dc.identifier.essn2045-2322
dc.identifier.pmcPMC8190102
dc.identifier.pmid34108558es_ES
dc.identifier.urihttp://hdl.handle.net/10668/4322
dc.journal.titleScientific Reports
dc.language.isoen
dc.page.number9 p.
dc.publisherNature Publishing Groupes_ES
dc.relation.publisherversionhttps://www.nature.com/articles/s41598-021-91505-8es_ES
dc.rightsAtribución 4.0 Internacional*
dc.rights.accessRightsAcceso abiertoes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectAmelogenines_ES
dc.subjectIncinerationes_ES
dc.subjectMolecular weightes_ES
dc.subjectDNA degradationes_ES
dc.subjectTeethes_ES
dc.subjectAmelogeninaes_ES
dc.subjectOdontología forensees_ES
dc.subjectIncineraciónes_ES
dc.subjectPeso moleculares_ES
dc.subjectFragmentación del ADNes_ES
dc.subjectDientees_ES
dc.subject.meshMedical Subject Headings::Persons::Persons::Age Groups::Adultes_ES
dc.subject.meshMedical Subject Headings::Persons::Persons::Age Groups::Adult::Agedes_ES
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Biometry::Biometric Identification::DNA Fingerprintinges_ES
dc.subject.meshMedical Subject Headings::Check Tags::Femalees_ES
dc.subject.meshMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humanses_ES
dc.subject.meshMedical Subject Headings::Check Tags::Malees_ES
dc.subject.meshMedical Subject Headings::Persons::Persons::Age Groups::Adult::Middle Agedes_ES
dc.subject.meshMedical Subject Headings::Anatomy::Stomatognathic System::Mouth::Dentition::Toothes_ES
dc.subject.meshMedical Subject Headings::Persons::Persons::Age Groups::Adult::Young Adultes_ES
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Physiological Phenomena::Physiological Processes::Stress, Physiological::Heat-Shock Responsees_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Dental Enamel Proteins::Amelogenines_ES
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Biological Phenomena::Ecological and Environmental Phenomena::Environment::Atmosphere::Weather::Temperaturees_ES
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Structures::Genome::Genome Components::Genes::Alleleses_ES
dc.subject.meshMedical Subject Headings::Anthropology, Education, Sociology and Social Phenomena::Social Sciences::Anthropology::Anthropology, Physical::Forensic Anthropologyes_ES
dc.subject.meshMedical Subject Headings::Health Care::Environment and Public Health::Public Health::Sanitation::Sanitary Engineering::Waste Management::Refuse Disposal::Incinerationes_ES
dc.subject.meshMedical Subject Headings::Health Care::Health Care Facilities, Manpower, and Services::Health Facilities::Laboratorieses_ES
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Chemical Phenomena::Physicochemical Phenomena::Molecular Weightes_ES
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Research Design::Reproducibility of Resultses_ES
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Photometry::Luminescent Measurements::Fluorometry::Spectrometry, Fluorescencees_ES
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reactiones_ES
dc.subject.meshMedical Subject Headings::Disciplines and Occupations::Natural Science Disciplines::Biological Science Disciplines::Biology::Computational Biology::Genomicses_ES
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Imaging::Image Interpretation, Computer-Assisted::Tomography, Emission-Computed::Positron-Emission Tomographyes_ES
dc.titleDNA degradation in human teeth exposed to thermal stresses_ES
dc.typeresearch article
dc.type.hasVersionVoR
dspace.entity.typePublication

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