Publication: Local Mesenchymal Stem Cell Therapy in Experimentally Induced Colitis in the Rat.
dc.contributor.author | de-la-Portilla, Fernando | |
dc.contributor.author | Yuste, Yaiza | |
dc.contributor.author | Pereira, Sheila | |
dc.contributor.author | Olano, Carolina | |
dc.contributor.author | Maestre, Maria Victoria | |
dc.contributor.author | Padillo, Francisco Javier | |
dc.contributor.funder | Nodo-Biobanco Hospital Virgen de Rocio-IBiS (Biobanco SSPA y Red Biobancos) | |
dc.date.accessioned | 2023-01-25T10:07:34Z | |
dc.date.available | 2023-01-25T10:07:34Z | |
dc.date.issued | 2018-05-30 | |
dc.description.abstract | Multipotent mesenchymal stem cells (MSCs) have been used in inflammatory bowel diseases because of their immunomodulatory and regenerative properties. We investigated their local use in an experimental model of colitis in the rat. Colitis was induced into 20 Wistar rats with local TNBS instillation. Allogeneic stem cells were derived from rat adipose tissue and labeled with PKH2 linker dye with creation of a control and a second group treated by a local injection into the rectal wall of 2×10⁶ allogeneic adipose tissue-derived stem cells (ADSCs). The thicknesses of different components of the rectum were measured with comparisons made in different parts of the colon of the Hunter inflammatory score. PKH2-dyed ADSCs were detected by fluorescence microscopy. Total colitis was induced in 19/20 rats with homing of fluorescent ADSCs. to the crypt base and perivascular space of the submucosa. There were no differences in component rectal wall thicknesses with a higher Hunter score in the treated group compared with the controls, in the rectum (3.8±2.74 vs. 1.5±2.37, respectively; p=0.017) and in right colon (2.5±1.08 vs. 0.20±0.42, respectively; p=0.0001). Local colonic injection of allogeneic adipose stem cells. in experimental colitis is feasible and safe. There is demonstrable homing of cells in chemically-induced colitis both to the treated region and parts of the colon distant to the MSC treatment site. Such cells readily proliferate in vitro and could potentially be a source for future treatment of resistant disease. | |
dc.description.version | Si | |
dc.identifier.citation | de la Portilla F, Yuste Y, Pereira S, Olano C, Maestre MV, Padillo FJ. Local Mesenchymal Stem Cell Therapy in Experimentally Induced Colitis in the Rat. Int J Stem Cells. 2018 May 30;11(1):39-47 | |
dc.identifier.doi | 10.15283/ijsc17074 | |
dc.identifier.issn | 2005-3606 | |
dc.identifier.pmc | PMC5984057 | |
dc.identifier.pmid | 29699385 | |
dc.identifier.pubmedURL | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5984057/pdf | |
dc.identifier.unpaywallURL | http://www.ijstemcell.com/journal/download_pdf.php?doi=10.15283/ijsc17074 | |
dc.identifier.uri | http://hdl.handle.net/10668/12401 | |
dc.issue.number | 1 | |
dc.journal.title | International journal of stem cells | |
dc.journal.titleabbreviation | Int J Stem Cells | |
dc.language.iso | en | |
dc.organization | Instituto de Biomedicina de Sevilla-IBIS | |
dc.organization | Hospital Universitario Virgen del Rocío | |
dc.page.number | 39-47 | |
dc.provenance | Realizada la curación de contenido 14/04/2025 | |
dc.publisher | Korean Society for Stem Cell Research,Han'Gug Julgi Sepo Haghoe | |
dc.pubmedtype | Journal Article | |
dc.relation.projectID | ISCIII-PT13/0010/0056 | |
dc.relation.publisherversion | http://www.ijstemcell.com/journal/DOIx.php?id=10.15283/ijsc17074 | |
dc.rights | Attribution-NonCommercial 4.0 International | |
dc.rights.accessRights | open access | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | |
dc.subject | Animal models | |
dc.subject | Colitis | |
dc.subject | Inflammatory bowel disease | |
dc.subject | Mesenchymal stem cells | |
dc.subject.decs | Colitis | |
dc.subject.decs | Células Madre | |
dc.subject.decs | Terapéutica | |
dc.subject.decs | Tejido adiposo | |
dc.subject.decs | Enfermedades Inflamatorias del Intestino | |
dc.subject.decs | Ratas Wistar | |
dc.subject.decs | Microscopía fluorescente | |
dc.subject.decs | Colorantes | |
dc.subject.decs | Predicción | |
dc.subject.mesh | Rats, Wistar | |
dc.subject.mesh | Rectum | |
dc.subject.mesh | Coloring Agents | |
dc.subject.mesh | Adipose Tissue | |
dc.subject.mesh | Models, Theoretical | |
dc.subject.mesh | Hematopoietic Stem Cell Transplantation | |
dc.title | Local Mesenchymal Stem Cell Therapy in Experimentally Induced Colitis in the Rat. | |
dc.type | research article | |
dc.type.hasVersion | VoR | |
dc.volume.number | 11 | |
dspace.entity.type | Publication |
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