Publication:
The human bone marrow harbors a CD45- CD11B+ cell progenitor permitting rapid microglia-like cell derivative approaches.

dc.contributor.authorBruzelius, Andreas
dc.contributor.authorHidalgo, Isabel
dc.contributor.authorBoza-Serrano, Antonio
dc.contributor.authorHjelmér, Anna-Giorgia
dc.contributor.authorTison, Amelie
dc.contributor.authorDeierborg, Tomas
dc.contributor.authorBengzon, Johan
dc.contributor.authorRamos-Moreno, Tania
dc.date.accessioned2023-02-09T10:38:09Z
dc.date.available2023-02-09T10:38:09Z
dc.date.issued2020-12-09
dc.description.abstractMicroglia, the immune sentinel of the central nervous system (CNS), are generated from yolk sac erythromyeloid progenitors that populate the developing CNS. Interestingly, a specific type of bone marrow-derived monocyte is able to express a yolk sac microglial signature and populate CNS in disease. Here we have examined human bone marrow (hBM) in an attempt to identify novel cell sources for generating microglia-like cells to use in cell-based therapies and in vitro modeling. We demonstrate that hBM stroma harbors a progenitor cell that we name stromal microglial progenitor (STR-MP). STR-MP single-cell gene analysis revealed the expression of the consensus genetic microglial signature and microglial-specific genes present in development and CNS pathologies. STR-MPs can be expanded and generate microglia-like cells in vitro, which we name stromal microglia (STR-M). STR-M cells show phagocytic ability, classically activate, and survive and phagocyte in human brain tissue. Thus, our results reveal that hBM harbors a source of microglia-like precursors that can be used in patient-centered fast derivative approaches.
dc.identifier.doi10.1002/sctm.20-0127
dc.identifier.essn2157-6580
dc.identifier.pmcPMC7980218
dc.identifier.pmid33295698
dc.identifier.pubmedURLhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7980218/pdf
dc.identifier.unpaywallURLhttps://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/sctm.20-0127
dc.identifier.urihttp://hdl.handle.net/10668/16762
dc.issue.number4
dc.journal.titleStem cells translational medicine
dc.journal.titleabbreviationStem Cells Transl Med
dc.language.isoen
dc.organizationInstituto de Biomedicina de Sevilla-IBIS
dc.organizationHospital Universitario Virgen del Rocío
dc.page.number582-597
dc.pubmedtypeJournal Article
dc.pubmedtypeResearch Support, Non-U.S. Gov't
dc.rightsAttribution 4.0 International
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectbone marrow
dc.subjectcommon myeloid progenitor
dc.subjecthuman bone marrow
dc.subjectmicroglia
dc.subjectmicroglia-like cell in vitro model
dc.subjectmicroglial precursor
dc.subjectpluripotent stem cell
dc.subjectprimitive myeloid progenitor
dc.subject.meshBone Marrow
dc.subject.meshCD11b Antigen
dc.subject.meshCentral Nervous System
dc.subject.meshHumans
dc.subject.meshLeukocyte Common Antigens
dc.subject.meshMicroglia
dc.subject.meshStem Cells
dc.titleThe human bone marrow harbors a CD45- CD11B+ cell progenitor permitting rapid microglia-like cell derivative approaches.
dc.typeresearch article
dc.type.hasVersionVoR
dc.volume.number10
dspace.entity.typePublication

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