Publication: Nuclear ingression of cytoplasmic bodies accompanies a boost in autophagy.
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Identifiers
Date
2022-05-13
Authors
Garcia, Manon
Kumanski, Sylvain
Elias-Villalobos, Alberto
Cazevieille, Chantal
Soulet, Caroline
Moriel-Carretero, Maria
Advisors
Journal Title
Journal ISSN
Volume Title
Publisher
Life Science Alliance LLC
Abstract
Membrane contact sites are functional nodes at which organelles reorganize metabolic pathways and adapt to changing cues. In Saccharomyces cerevisiae, the nuclear envelope subdomain surrounding the nucleolus, very plastic and prone to expansion, can establish contacts with the vacuole and be remodeled in response to various metabolic stresses. While using genotoxins with unrelated purposes, we serendipitously discovered a fully new remodeling event at this nuclear subdomain: the nuclear envelope partitions into its regular contact with the vacuole and a dramatic internalization within the nucleus. This leads to the nuclear engulfment of a globular, cytoplasmic portion. In spite of how we discovered it, the phenomenon is likely DNA damage-independent. We define lipids supporting negative curvature, such as phosphatidic acid and sterols, as bona fide drivers of this event. Mechanistically, we suggest that the engulfment of the cytoplasm triggers a suction phenomenon that enhances the docking of proton pump-containing vesicles with the vacuolar membrane, which we show matches a boost in autophagy. Thus, our findings unveil an unprecedented remodeling of the nucleolus-surrounding membranes with impact on metabolic adaptation.
Description
MeSH Terms
Autophagy
Cytoplasm
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Vacuoles
Cytoplasm
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Vacuoles
DeCS Terms
sitios de contacto membrana
unión núcleo‑vacuola
ácido fosfatídico
esteroles
autofagia
saccharomyces cerevisiae
unión núcleo‑vacuola
ácido fosfatídico
esteroles
autofagia
saccharomyces cerevisiae
CIE Terms
Keywords
Membrane Contact Sites, Nucleus–Vacuole Junction, Phosphatidic Acid, Sterols, Autophagy, Saccharomyces cerevisiae
Citation
Garcia M, Kumanski S, Elías-Villalobos A, Cazevieille C, Soulet C, Moriel-Carretero M. Nuclear ingression of cytoplasmic bodies accompanies a boost in autophagy. Life Sci Alliance. 2022 May 13;5(9):e202101160.