Publication:
Gut Microbiota Composition Is Associated With the Global DNA Methylation Pattern in Obesity.

dc.contributor.authorRamos-Molina, Bruno
dc.contributor.authorSánchez-Alcoholado, Lidia
dc.contributor.authorCabrera-Mulero, Amanda
dc.contributor.authorLopez-Dominguez, Raul
dc.contributor.authorCarmona-Saez, Pedro
dc.contributor.authorGarcia-Fuentes, Eduardo
dc.contributor.authorMoreno-Indias, Isabel
dc.contributor.authorTinahones, Francisco J
dc.contributor.funderCentros de Investigación Biomédica en Red
dc.contributor.funderInstitute of Health Carlos III
dc.contributor.funderEuropean Regional Development Fund
dc.date.accessioned2023-01-25T13:37:23Z
dc.date.available2023-01-25T13:37:23Z
dc.date.issued2019-07-03
dc.description.abstractObjective: Obesity and obesity-related metabolic diseases are characterized by gut microbiota and epigenetic alterations. Recent insight has suggested the existence of a crosstalk between the gut microbiome and the epigenome. However, the possible link between alterations in gut microbiome composition and epigenetic marks in obesity has been not explored yet. The aim of this work is to establish a link between the gut microbiota and the global DNA methylation profile in a group of obese subjects and to report potential candidate genes that could be epigenetically regulated by gut microbiota in adipose tissue. Methods: Gut microbiota composition was analyzed in DNA stool samples from 45 obese subjects by 16S ribosomal RNA (rRNA) gene sequencing. Twenty patients were selected based on their Bacteroidetes-to-Firmicutes ratio (BFR): HighBFR group (BFR > 2.5, n = 10) and LowBFR group (BFR < 1.2, n = 10). Genome-wide analysis of DNA methylation pattern in both whole blood and visceral adipose tissue of these selected patients was performed with an Infinium EPIC BeadChip array-based platform. Gene expression analysis of candidate genes was done in adipose tissue by real-time quantitative PCR. Results: Genome-wide analysis of DNA methylation revealed a completely different DNA methylome pattern in both blood and adipose tissue in the low BFR group vs. the high BFR group. Two hundred fifty-eight genes were differentially methylated in both blood and adipose tissue, of which several potential candidates were selected for gene expression analysis. We found that in adipose tissue, both HDAC7 and IGF2BP2 were hypomethylated and overexpressed in the low BFR group compared with the high BFR group. β values of both genes significantly correlated with the BFR ratio and the relative abundance of Bacteroidetes and/or Firmicutes. Conclusions: In this study, we demonstrate that the DNA methylation status is associated with gut microbiota composition in obese subjects and that the expression levels of candidate genes implicated in glucose and energy homeostasis (e.g., HDAC7 and IGF2BP2) could be epigenetically regulated by gut bacterial populations in adipose tissue.
dc.description.sponsorshipThis study was supported by the “Centros de Investigación Biomédica en Red” (CIBER) of the Institute of Health Carlos III (ISCIII) (CB06/03/0018) and research grants from the ISCIII (grant numbers PI15/01114 and PI18/01160) and co-financed by the European Regional Development Fund (ERDF). BR-M was a recipient of a Sara Borrell postdoctoral fellowship from the ISCIII (CD16/0003) and co-funded by the ERDF. IM-I was supported by the Miguel Servet Type I program (CP16/00163) from the ISCIII and co-funded by the ERDF.
dc.description.version
dc.identifier.citationRamos-Molina B, Sánchez-Alcoholado L, Cabrera-Mulero A, Lopez-Dominguez R, Carmona-Saez P, Garcia-Fuentes E, et al. Gut Microbiota Composition Is Associated With the Global DNA Methylation Pattern in Obesity. Front Genet. 2019;10:613. Published 2019 Jul 3.
dc.identifier.doi10.3389/fgene.2019.00613
dc.identifier.issn1664-8021
dc.identifier.pmcPMC6616130
dc.identifier.pmid31333715
dc.identifier.pubmedURLhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6616130/pdf
dc.identifier.unpaywallURLhttps://europepmc.org/articles/pmc6616130?pdf=render
dc.identifier.urihttp://hdl.handle.net/10668/14288
dc.journal.titleFrontiers in genetics
dc.journal.titleabbreviationFront Genet
dc.language.isoen
dc.organizationHospital Universitario Virgen de la Victoria
dc.organizationInstituto de Investigación Biomédica de Málaga-IBIMA
dc.page.number9
dc.provenanceRealizada la curación de contenido 25/06/2025
dc.publisherFrontiers
dc.pubmedtypeJournal Article
dc.relation.projectIDCB06/03/0018
dc.relation.projectIDPI15/01114
dc.relation.projectIDPI18/01160
dc.relation.projectIDCD16/0003
dc.relation.projectIDCP16/00163
dc.relation.publisherversionhttps://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2019.00613/full
dc.rightsAttribution 4.0 International
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectadipose tissue
dc.subjectepigenetics
dc.subjectgut microbiota
dc.subjectmethylation
dc.subjectobesity
dc.subject.decsMicrobioma Gastrointestinal
dc.subject.decsGenes
dc.subject.decsTejido Adiposo
dc.subject.decsMetilación de ADN
dc.subject.decsEpigenoma
dc.subject.decsBacteroidetes
dc.subject.decsGlucosa
dc.subject.decsGrasa Intraabdominal
dc.subject.decsADN
dc.subject.decsARN Ribosómico 16S
dc.subject.meshGastrointestinal Microbiome
dc.subject.meshEpigenome
dc.subject.meshRNA, Ribosomal, 16S
dc.subject.meshDNA Methylation
dc.subject.meshFirmicutes
dc.subject.meshBacteroidetes
dc.subject.meshGenes, rRNA
dc.subject.meshIntra-Abdominal Fat
dc.subject.meshDNA
dc.subject.meshEpigenesis, Genetic
dc.titleGut Microbiota Composition Is Associated With the Global DNA Methylation Pattern in Obesity.
dc.typeresearch article
dc.type.hasVersionVoR
dc.volume.number10
dspace.entity.typePublication

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
PMC6616130.pdf
Size:
1.55 MB
Format:
Adobe Portable Document Format