In vitro effect of qnrA1, qnrB1, and qnrS1 genes on fluoroquinolone activity against isogenic Escherichia coli isolates with mutations in gyrA and parC.

Briales, A; Rodríguez-Martínez, J M; Velasco, C; Díaz de Alba, P; Domínguez-Herrera, J; Pachón, J; Pascual, A

Publication:
In vitro effect of qnrA1, qnrB1, and qnrS1 genes on fluoroquinolone activity against isogenic Escherichia coli isolates with mutations in gyrA and parC.

dc.contributor.author	Briales, A
dc.contributor.author	Rodríguez-Martínez, J M
dc.contributor.author	Velasco, C
dc.contributor.author	Díaz de Alba, P
dc.contributor.author	Domínguez-Herrera, J
dc.contributor.author	Pachón, J
dc.contributor.author	Pascual, A
dc.contributor.authoraffiliation	[Briales,A; Rodríguez-MartínezJM; Velasco,C; Díaz de Alba,P; Pascual,A] Department of Microbiology, University of Seville, Seville, Spain. [Domínguez-Herrera,J; Pachón,J] Infectious Diseases Service, Biomedicine Institute of Seville (IBiS), University Hospitals Virgen del Rocío/CSIC/University of Seville, Seville, Spain. [Pascual,A] University Hospital Virgen Macarena, Seville, Spain.	es
dc.contributor.funder	This work was supported by the Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III (project PI060580) and the Consejería de Innovación Ciencia y Empresa, Junta de Andalucía (P07-CTS-02908), Spain. It was partly supported by the Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III-FEDER, Spanish Network for Research in Infectious Diseases (REIPI RD06/0008). A.B. was funded by a predoctoral grant from the Instituto de Salud Carlos III (PFIS), Spain.
dc.date.accessioned	2016-08-10T10:52:10Z
dc.date.available	2016-08-10T10:52:10Z
dc.date.issued	2011-03
dc.description	Journal Article; Research Support, Non-U.S. Gov't;	es
dc.description.abstract	This article provides an analysis of the in vitro effect of qnrA1, qnrB1, and qnrS1 genes, combined with quinolone-resistant Ser83Leu substitutions in GyrA and/or Ser80Arg in ParC, on fluoroquinolone (FQ) resistance in isogenic Escherichia coli strains. The association of Ser83Leu substitution in GyrA, Ser80Arg substitution in ParC, and qnr gene expression increased the MIC of ciprofloxacin to 2 μg/ml. qnr genes present in E. coli that harbored a Ser83Leu substitution in GyrA increased mutant prevention concentration (MPC) values to 8 to 32 μg/ml. qnr gene expression in E. coli may play an important role in selecting for one-step FQ-resistant mutants.	es
dc.description.version	Yes	es
dc.identifier.citation	Briales A, Rodríguez-Martínez JM, Velasco C, Díaz de Alba P, Domínguez-Herrera J, Pachón J, et al. In vitro effect of qnrA1, qnrB1, and qnrS1 genes on fluoroquinolone activity against isogenic Escherichia coli isolates with mutations in gyrA and parC. Antimicrob. Agents Chemother. 2011; 55(3):1266-9	es
dc.identifier.doi	10.1128/AAC.00927-10
dc.identifier.essn	1098-6596
dc.identifier.issn	0066-4804
dc.identifier.pmc	PMC3067081
dc.identifier.pmid	21173174
dc.identifier.uri	http://hdl.handle.net/10668/2364
dc.journal.title	Antimicrobial Agents and Chemotherapy
dc.language.iso	en
dc.publisher	American Society for Microbiology	es
dc.relation.publisherversion	http://aac.asm.org/content/55/3/1266.abstract	es
dc.rights.accessRights	open access
dc.subject	Pruebas de sensibilidad microbiana	es
dc.subject	Mutación	es
dc.subject	Ciprofloxacino	es
dc.subject.mesh	Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Isomerases::DNA Topoisomerases::DNA Topoisomerases, Type II::DNA Gyrase	es
dc.subject.mesh	Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Isomerases::DNA Topoisomerases::DNA Topoisomerases, Type II::DNA Topoisomerase IV	es
dc.subject.mesh	Medical Subject Headings::Organisms::Bacteria::Gram-Negative Bacteria::Gram-Negative Facultatively Anaerobic Rods::Enterobacteriaceae::Escherichia::Escherichia coli	es
dc.subject.mesh	Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Bacterial Proteins::Escherichia coli Proteins	es
dc.subject.mesh	Medical Subject Headings::Chemicals and Drugs::Heterocyclic Compounds::Heterocyclic Compounds, 2-Ring::Quinolines::Quinolones::Fluoroquinolones	es
dc.subject.mesh	Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Microbiological Techniques::Microbial Sensitivity Tests	es
dc.subject.mesh	Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Variation::Mutation	es
dc.subject.mesh	Medical Subject Headings::Chemicals and Drugs::Heterocyclic Compounds::Heterocyclic Compounds, 2-Ring::Quinolines::Quinolones::Fluoroquinolones::Ciprofloxacin	es
dc.title	In vitro effect of qnrA1, qnrB1, and qnrS1 genes on fluoroquinolone activity against isogenic Escherichia coli isolates with mutations in gyrA and parC.	es
dc.type	research article
dc.type.hasVersion	VoR
dspace.entity.type	Publication

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Publication: In vitro effect of qnrA1, qnrB1, and qnrS1 genes on fluoroquinolone activity against isogenic Escherichia coli isolates with mutations in gyrA and parC.

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Publication:
In vitro effect of qnrA1, qnrB1, and qnrS1 genes on fluoroquinolone activity against isogenic Escherichia coli isolates with mutations in gyrA and parC.