Publication:
A Quantitative Digital Analysis of Tissue Immune Components Reveals an Immunosuppressive and Anergic Immune Response with Relevant Prognostic Significance in Glioblastoma.

dc.contributor.authorIdoate Gastearena, Miguel A
dc.contributor.authorLópez-Janeiro, Álvaro
dc.contributor.authorLecumberri Aznarez, Arturo
dc.contributor.authorArana-Iñiguez, Iñigo
dc.contributor.authorGuillén-Grima, Francisco
dc.date.accessioned2023-05-03T13:48:50Z
dc.date.available2023-05-03T13:48:50Z
dc.date.issued2022-07-21
dc.description.abstractObjectives: Immunostimulatory therapies using immune checkpoint blockers show clinical activity in a subset of glioblastoma (GBM) patients. Several inhibitory mechanisms play a relevant role in the immune response to GBM. With the objective of analyzing the tumor immune microenvironment and its clinical significance, we quantified several relevant immune biomarkers. Design: We studied 76 primary (non-recurrent) GBMs with sufficient clinical follow-up, including a subgroup of patients treated with a dendritic cell vaccine. The IDH-mutation, EGFR-amplification, and MGMT methylation statuses were determined. Several relevant immune biomarkers, including CD163, CD8, PD1, and PDL1, were quantified in representative selected areas by digital image analysis and semiquantitative evaluation. The percentage of each immune expression was calculated with respect to the total number of tumor cells. Results: All GBMs were wild-type IDH, with a subgroup of classical GBMs according to the EGFR amplification (44%). Morphologically, CD163 immunostained microglia and intratumor clusters of macrophages were observed. A significant direct correlation was found between the expression of CD8 and the mechanisms of lymphocyte immunosuppression, in such a way that higher values of CD8 were directly associated with higher values of CD163 (p
dc.identifier.doi10.3390/biomedicines10071753
dc.identifier.issn2227-9059
dc.identifier.pmcPMC9313250
dc.identifier.pmid35885058
dc.identifier.pubmedURLhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313250/pdf
dc.identifier.unpaywallURLhttps://www.mdpi.com/2227-9059/10/7/1753/pdf?version=1658455182
dc.identifier.urihttp://hdl.handle.net/10668/20844
dc.issue.number7
dc.journal.titleBiomedicines
dc.journal.titleabbreviationBiomedicines
dc.language.isoen
dc.organizationHospital Universitario Virgen Macarena
dc.organizationHospital Universitario Virgen Macarena
dc.pubmedtypeJournal Article
dc.rightsAttribution 4.0 International
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectM2 macrophage
dc.subjectglioblastoma
dc.subjectimage analysis
dc.subjectimmune barrier
dc.subjectimmunotherapy
dc.subjectmicroglia
dc.titleA Quantitative Digital Analysis of Tissue Immune Components Reveals an Immunosuppressive and Anergic Immune Response with Relevant Prognostic Significance in Glioblastoma.
dc.typeresearch article
dc.type.hasVersionVoR
dc.volume.number10
dspace.entity.typePublication

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