Publication:
Poly(ethylmethacrylate-co-diethylaminoethyl acrylate) coating improves endothelial re-population, bio-mechanical and anti-thrombogenic properties of decellularized carotid arteries for blood vessel replacement.

dc.contributor.authorLópez-Ruiz, Elena
dc.contributor.authorVenkateswaran, Seshasailam
dc.contributor.authorPerán, Macarena
dc.contributor.authorJiménez, Gema
dc.contributor.authorPernagallo, Salvatore
dc.contributor.authorDíaz-Mochón, Juan J
dc.contributor.authorTura-Ceide, Olga
dc.contributor.authorArrebola, Francisco
dc.contributor.authorMelchor, Juan
dc.contributor.authorSoto, Juan
dc.contributor.authorRus, Guillermo
dc.contributor.authorReal, Pedro J
dc.contributor.authorDiaz-Ricart, María
dc.contributor.authorConde-González, Antonio
dc.contributor.authorBradley, Mark
dc.contributor.authorMarchal, Juan A
dc.date.accessioned2023-01-25T09:44:21Z
dc.date.available2023-01-25T09:44:21Z
dc.date.issued2017-03-24
dc.description.abstractDecellularized vascular scaffolds are promising materials for vessel replacements. However, despite the natural origin of decellularized vessels, issues such as biomechanical incompatibility, immunogenicity risks and the hazards of thrombus formation, still need to be addressed. In this study, we coated decellularized vessels obtained from porcine carotid arteries with poly (ethylmethacrylate-co-diethylaminoethylacrylate) (8g7) with the purpose of improving endothelial coverage and minimizing platelet attachment while enhancing the mechanical properties of the decellularized vascular scaffolds. The polymer facilitated binding of endothelial cells (ECs) with high affinity and also induced endothelial cell capillary tube formation. In addition, platelets showed reduced adhesion on the polymer under flow conditions. Moreover, the coating of the decellularized arteries improved biomechanical properties by increasing its tensile strength and load. In addition, after 5 days in culture, ECs seeded on the luminal surface of 8g7-coated decellularized arteries showed good regeneration of the endothelium. Overall, this study shows that polymer coating of decellularized vessels provides a new strategy to improve re-endothelialization of vascular grafts, maintaining or enhancing mechanical properties while reducing the risk of thrombogenesis. These results could have potential applications in improving tissue-engineered vascular grafts for cardiovascular therapies with small caliber vessels.
dc.identifier.doi10.1038/s41598-017-00294-6
dc.identifier.essn2045-2322
dc.identifier.pmcPMC5412652
dc.identifier.pmid28341826
dc.identifier.pubmedURLhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412652/pdf
dc.identifier.unpaywallURLhttps://www.nature.com/articles/s41598-017-00294-6.pdf
dc.identifier.urihttp://hdl.handle.net/10668/11004
dc.issue.number1
dc.journal.titleScientific reports
dc.journal.titleabbreviationSci Rep
dc.language.isoen
dc.organizationCentro Pfizer-Universidad de Granada-Junta de Andalucía de Genómica e Investigación Oncológica-GENYO
dc.page.number407
dc.pubmedtypeJournal Article
dc.pubmedtypeResearch Support, Non-U.S. Gov't
dc.rightsAttribution 4.0 International
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.meshAnimals
dc.subject.meshBiocompatible Materials
dc.subject.meshBiopolymers
dc.subject.meshBlood Platelets
dc.subject.meshBlood Vessel Prosthesis
dc.subject.meshCarotid Arteries
dc.subject.meshCarotid Artery Thrombosis
dc.subject.meshEndothelial Cells
dc.subject.meshEndothelium, Vascular
dc.subject.meshHuman Umbilical Vein Endothelial Cells
dc.subject.meshHumans
dc.subject.meshMethylmethacrylates
dc.subject.meshSwine
dc.subject.meshTissue Engineering
dc.subject.meshTissue Scaffolds
dc.titlePoly(ethylmethacrylate-co-diethylaminoethyl acrylate) coating improves endothelial re-population, bio-mechanical and anti-thrombogenic properties of decellularized carotid arteries for blood vessel replacement.
dc.typeresearch article
dc.type.hasVersionVoR
dc.volume.number7
dspace.entity.typePublication

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