Publication:
Direct identification of microorganisms from thioglycolate broth by MALDI-TOF MS.

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dc.contributor.authorArca-Suárez, Jorge
dc.contributor.authorGalán-Sánchez, Fátima
dc.contributor.authorMarin-Casanova, Pilar
dc.contributor.authorRodríguez-Iglesias, Manuel A
dc.date.accessioned2023-01-25T09:52:29Z
dc.date.available2023-01-25T09:52:29Z
dc.date.issued2017-09-21
dc.description.abstractWe developed an easy MALDI-TOF MS-based assay to identify microorganisms directly from thioglycolate broth. A total of 101 positive thioglycolate broths inoculated with 15 different kinds of samples were evaluated. In 91 samples (90.1%), direct MALDI-TOF MS identifications were the same as those obtained after conventional laboratory procedures including subcultures. In 10 samples misidentified by direct processing, yeasts or mixed cultures grew in the thioglycolate subcultures, or high cellular debris hampered a correct analysis. This rapid method can provide a fast, clinically- relevant species-level identification without disturbing the daily workflow in clinical microbiology laboratories.
dc.identifier.doi10.1371/journal.pone.0185229
dc.identifier.essn1932-6203
dc.identifier.pmcPMC5608331
dc.identifier.pmid28934331
dc.identifier.pubmedURLhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5608331/pdf
dc.identifier.unpaywallURLhttps://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0185229&type=printable
dc.identifier.urihttp://hdl.handle.net/10668/11599
dc.issue.number9
dc.journal.titlePloS one
dc.journal.titleabbreviationPLoS One
dc.language.isoen
dc.organizationHospital Universitario Puerta del Mar
dc.page.numbere0185229
dc.pubmedtypeJournal Article
dc.rightsAttribution 4.0 International
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.meshBacteria
dc.subject.meshCandida albicans
dc.subject.meshCulture Media
dc.subject.meshLaboratories
dc.subject.meshSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
dc.subject.meshThioglycolates
dc.titleDirect identification of microorganisms from thioglycolate broth by MALDI-TOF MS.
dc.typeresearch article
dc.type.hasVersionVoR
dc.volume.number12
dspace.entity.typePublication

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