Publication:
miR-30b-5p Downregulation as a Predictive Biomarker of Coronary In-Stent Restenosis.

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Date

2021-03-30

Authors

Gutierrez-Carretero, Encarnacion
Mayoral-Gonzalez, Isabel
Jesus Moron, Francisco
Fernandez-Quero, Monica
Dominguez-Rodriguez, Alejandro
Ordoñez, Antonio
Smani, Tarik

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MDPI AG
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Abstract

In-stent restenosis (ISR) is one of the main limitations of percutaneous coronary intervention (PCI) therapy with drug-eluting stents (DES) implantation. The aim of this study was to determine if circulating microRNAs (miRNAs) have diagnostic capability for determining ISR in a cohort of matched patients. Blood samples were collected from 55 patients who underwent previously PCI and were readmitted for a new coronary angiography. Patients were divided into subgroups comprising patients who presented ISR or not (non-ISR). A microarray analysis determined that up to 49 miRNAs were differentially expressed between ISR and non-ISR patients. Of these, 10 miRNAs are related to vascular smooth muscle and endothelial cells proliferation, migration, and differentiation, well-known hallmarks of vascular remodeling. Additionally, we identified that the expression of miR-30b-5p is significantly lower in serum samples of ISR patients, as compared to non-ISR. A further analysis demonstrated that miR-30b-5p provides better values of the receiver operator characteristic curve than other miRNAs and biochemical parameters. Finally, the in-silico analysis suggests that miR-30b-5p is predicted to target 62 genes involved in different signaling pathways involved in vascular remodeling. In conclusion, we determined for the first time that circulating mi-R30b-5p can reliably prognose restenosis in patient with implanted DES, which could be potentially helpful in the establishment of an early diagnosis and therapy of ISR.

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MeSH Terms

MicroRNAs
Drug-Eluting Stents
Coronary Angiography
Percutaneous Coronary Intervention
Circulating MicroRNA
Muscle, Smooth, Vascular
Coronary Restenosis
Endothelial Cells
Vascular Remodeling
Prognosis
Microarray Analysis

DeCS Terms

Pacientes
MicroARNs
Remodelación vascular
Angiografía coronaria
Stents liberadores de fármacos
Genes
Análisis por micromatrices
Stents
Suero

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Keywords

Primary Coronary Intervention, biomarker, in-stent restenosis, miRNAs

Citation

Gutierrez-Carretero E, Mayoral-González I, Jesús Morón F, Fernández-Quero M, Domínguez-Rodríguez A, Ordóñez A, et al. miR-30b-5p Downregulation as a Predictive Biomarker of Coronary In-Stent Restenosis. Biomedicines. 2021 Mar 30;9(4):354