Publication: Discovery of a Novel, Isothiazolonaphthoquinone-Based Small Molecule Activator of FOXO Nuclear-Cytoplasmic Shuttling
dc.contributor.author | Cautain, Bastien | |
dc.contributor.author | Castillo, Francisco | |
dc.contributor.author | Musso, Loana | |
dc.contributor.author | Ferreira, Bibiana I. | |
dc.contributor.author | de Pedro, Nuria | |
dc.contributor.author | Quesada, Lorena Rodriguez | |
dc.contributor.author | Machado, Susana | |
dc.contributor.author | Vicente, Francisca | |
dc.contributor.author | Dallavalle, Sabrina | |
dc.contributor.author | Link, Wolfgang | |
dc.contributor.authoraffiliation | [Cautain, Bastien] Fdn MEDINA, Parque Tecnol Ciencias Salud, Granada, Spain | |
dc.contributor.authoraffiliation | [Castillo, Francisco] Fdn MEDINA, Parque Tecnol Ciencias Salud, Granada, Spain | |
dc.contributor.authoraffiliation | [de Pedro, Nuria] Fdn MEDINA, Parque Tecnol Ciencias Salud, Granada, Spain | |
dc.contributor.authoraffiliation | [Quesada, Lorena Rodriguez] Fdn MEDINA, Parque Tecnol Ciencias Salud, Granada, Spain | |
dc.contributor.authoraffiliation | [Vicente, Francisca] Fdn MEDINA, Parque Tecnol Ciencias Salud, Granada, Spain | |
dc.contributor.authoraffiliation | [Musso, Loana] Univ Milan, DeFENS Dept Food Environm & Nutr Sci, I-20122 Milan, Italy | |
dc.contributor.authoraffiliation | [Dallavalle, Sabrina] Univ Milan, DeFENS Dept Food Environm & Nutr Sci, I-20122 Milan, Italy | |
dc.contributor.authoraffiliation | [Ferreira, Bibiana I.] Ctr Biomed Res CBMR, Gambelas Campus, Faro, Portugal | |
dc.contributor.authoraffiliation | [Machado, Susana] Ctr Biomed Res CBMR, Gambelas Campus, Faro, Portugal | |
dc.contributor.authoraffiliation | [Link, Wolfgang] Ctr Biomed Res CBMR, Gambelas Campus, Faro, Portugal | |
dc.contributor.authoraffiliation | [Ferreira, Bibiana I.] Univ Algarve, Dept Biomed Sci & Med, Regenerat Med Program, Campus Gambelas, Faro, Portugal | |
dc.contributor.authoraffiliation | [Link, Wolfgang] Univ Algarve, Dept Biomed Sci & Med, Regenerat Med Program, Campus Gambelas, Faro, Portugal | |
dc.contributor.funder | Fundacao para a Ciencia e a Tecnologia (FCT) Research Center | |
dc.contributor.funder | FCT | |
dc.contributor.funder | ProRegem grant | |
dc.date.accessioned | 2023-02-12T02:22:21Z | |
dc.date.available | 2023-02-12T02:22:21Z | |
dc.date.issued | 2016-12-09 | |
dc.description.abstract | FOXO factors are tumour suppressor proteins commonly inactivated in human tumours by posttranslational modifications. Furthermore, genetic variation within the FOXO3a gene is consistently associated with human longevity. Therefore, the pharmacological activation of FOXO proteins is considered as an attractive therapeutic approach to treat cancer and age-related diseases. In order to identify agents capable of activating FOXOs, we tested a collection of small chemical compounds using image-based high content screening technology. Here, we report the discovery of LOM612 (compound 1a), a newly synthesized isothiazolonaphthoquinone as a potent FOXO relocator. Compound 1a induces nuclear translocation of a FOXO3a reporter protein as well as endogenous FOXO3a and FOXO1 in U2OS cells in a dose-dependent manner. This activity does not affect the subcellular localization of other cellular proteins including NFkB or inhibit CRM1-mediated nuclear export. Furthermore, compound 1a shows a potent antiproliferative effect in human cancer cell lines. | |
dc.identifier.doi | 10.1371/journal.pone.0167491 | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.unpaywallURL | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0167491&type=printable | |
dc.identifier.uri | http://hdl.handle.net/10668/19166 | |
dc.identifier.wosID | 389587100109 | |
dc.issue.number | 12 | |
dc.journal.title | Plos one | |
dc.journal.titleabbreviation | Plos one | |
dc.language.iso | en | |
dc.organization | Fundación MEDINA (Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía) | |
dc.organization | Fundación MEDINA | |
dc.publisher | Public library science | |
dc.rights | Attribution 4.0 International | |
dc.rights.accessRights | open access | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Forkhead transcription factor | |
dc.subject | Dual-specificity phosphatase | |
dc.subject | Cycloaddition reactions | |
dc.subject | Nitrile sulfides | |
dc.subject | Inhibitors | |
dc.subject | Cancer | |
dc.subject | Translocation | |
dc.subject | Screen | |
dc.subject | Potent | |
dc.subject | Cells | |
dc.title | Discovery of a Novel, Isothiazolonaphthoquinone-Based Small Molecule Activator of FOXO Nuclear-Cytoplasmic Shuttling | |
dc.type | research article | |
dc.type.hasVersion | VoR | |
dc.volume.number | 11 | |
dc.wostype | Article | |
dspace.entity.type | Publication |