Publication:
The Roles of Imprinted SLC22A18 and SLC22A18AS Gene Overexpression Caused by Promoter CpG Island Hypomethylation as Diagnostic and Prognostic Biomarkers for Non-Small Cell Lung Cancer Patients

dc.contributor.authorNoguera-Uclés, José Francisco
dc.contributor.authorBoyero, Laura
dc.contributor.authorSalinas, Ana
dc.contributor.authorCordero Varela, Juan Antonio
dc.contributor.authorBenedetti, Johana Cristina
dc.contributor.authorBernabé-Caro, Reyes
dc.contributor.authorSánchez-Gastaldo, Amparo
dc.contributor.authorAlonso, Miriam
dc.contributor.authorPaz-Ares, Luis
dc.contributor.authorMolina-Pinelo, Sonia
dc.contributor.authoraffiliation[Noguera-Uclés,JF; Boyero,L; Salinas,A; Cordero Varela,JA; Benedetti,JC; Bernabé-Caro,R; Sánchez-Gastaldo,A; Alonso,M; Molina-Pinelo,S] Institute of Biomedicine of Seville (IBiS) (HUVR, CSIC, Universidad de Sevilla), Seville, Spain. [Benedetti,JC; Bernabé-Caro,R; Sánchez-Gastaldo,A; Alonso,M; Molina-Pinelo,S] Medical Oncology Department, Hospital Universitario Virgen del Rocío, Seville, Spain. [Paz-Ares,L; Molina-Pinelo,S] Centro de Investigación Biomédica en Red de Cáncer (CIBERONC), Madrid, Spain. [Paz-Ares,L] H12O-CNIO Lung Cancer Clinical Research Unit, Instituto de Investigación Hospital 12 de Octubre & Centro Nacional de Investigaciones Oncológicas (CNIO), Madrid, Spain. [Paz-Ares,L] Medical Oncology Department, Hospital Universitario 12 de Octubre, Madrid, Spain.
dc.contributor.funderThis research was funded by the Ministry of Health and Social Welfare of Junta de Andalucía (PI-0046-2012, Nicolas Monardes Program C-0040-2016), and Instituto de Salud Carlos III though the project PI17/00033 (Co-funded by European Regional Development Fund) "A way to make Europe".
dc.date.accessioned2022-09-27T12:18:26Z
dc.date.available2022-09-27T12:18:26Z
dc.date.issued2020-07-27
dc.description.abstractGenomic imprinting is a process that involves one gene copy turned-off in a parent-of-origin-dependent manner. The regulation of imprinted genes is broadly dependent on promoter methylation marks, which are frequently associated with both oncogenes and tumor suppressors. The purpose of this study was to assess the DNA methylation patterns of the imprinted solute-carrier family 22 member 18 (SLC22A18) and SLC22A18 antisense (SLC22A18AS) genes in non-small cell lung cancer (NSCLC) patients to study their relevance to the disease. We found that both genes were hypomethylated in adenocarcinoma and squamous cell carcinoma patients. Due to this imprinting loss, SLC22A18 and SLC22A18AS were found to be overexpressed in NSCLC tissues, which is significantly more evident in lung adenocarcinoma patients. These results were validated through analyses of public databases of NSCLC patients. The reversed gene profile of both genes was achieved in vitro by treatment with ademetionine. We then showed that high SLC22A18 and SLC22A18AS expression levels were significantly associated with worsening disease progression. In addition, low levels of SLC22A18AS were also correlated with better overall survival for lung adenocarcinoma patients. We found that SLC22A18 and SLC22A18AS knockdown inhibits cell proliferation in vitro. All these results suggest that both genes may be useful as diagnostic and prognostic biomarkers in NSCLC, revealing novel therapeutic opportunities.es_ES
dc.description.versionYeses_ES
dc.identifier.citationNoguera-Uclés JF, Boyero L, Salinas A, Cordero Varela JA, Benedetti JC, Bernabé-Caro R, et al. The Roles of Imprinted SLC22A18 and SLC22A18AS Gene Overexpression Caused by Promoter CpG Island Hypomethylation as Diagnostic and Prognostic Biomarkers for Non-Small Cell Lung Cancer Patients. Cancers. 2020 Jul 27;12(8):2075es_ES
dc.identifier.doi10.3390/cancers12082075es_ES
dc.identifier.essn2072-6694
dc.identifier.pmcPMC7466018
dc.identifier.pmid32726996es_ES
dc.identifier.urihttp://hdl.handle.net/10668/4166
dc.journal.titleCancers
dc.language.isoen
dc.page.number18 p.
dc.publisherMDPIes_ES
dc.relation.publisherversionhttps://www.mdpi.com/2072-6694/12/8/2075/htmes_ES
dc.rightsAtribución 4.0 Internacional*
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectNSCLCes_ES
dc.subjectSLC22A18es_ES
dc.subjectIMPT1es_ES
dc.subjectTSSC5es_ES
dc.subjectSLC22A18ASes_ES
dc.subjectGenomic imprintinges_ES
dc.subjectPrognosises_ES
dc.subjectDiagnostices_ES
dc.subjectBiomarkerses_ES
dc.subjectMethylationes_ES
dc.subjectOncogeneses_ES
dc.subjectLung canceres_ES
dc.subjectImpresión genómicaes_ES
dc.subjectDiagnósticoes_ES
dc.subjectBiomarcadoreses_ES
dc.subjectMetilaciónes_ES
dc.subjectNeoplasias pulmonareses_ES
dc.subject.meshMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humanses_ES
dc.subject.meshMedical Subject Headings::Diseases::Neoplasms::Neoplasms by Site::Thoracic Neoplasms::Respiratory Tract Neoplasms::Lung Neoplasms::Bronchial Neoplasms::Carcinoma, Bronchogenic::Carcinoma, Non-Small-Cell Lunges_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Organic Chemicals::Sulfur Compounds::Amino Acids, Sulfur::Methionine::S-Adenosylmethioninees_ES
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression Regulation::Epigenesis, Genetic::Genomic Imprintinges_ES
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::DNA Methylationes_ES
dc.subject.meshMedical Subject Headings::Diseases::Neoplasms::Neoplasms by Site::Thoracic Neoplasms::Respiratory Tract Neoplasms::Lung Neoplasmses_ES
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Prognosises_ES
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Growth Processes::Cell Proliferationes_ES
dc.subject.meshMedical Subject Headings::Diseases::Pathological Conditions, Signs and Symptoms::Pathologic Processes::Disease Attributes::Disease Progressiones_ES
dc.subject.meshMedical Subject Headings::Diseases::Neoplasms::Neoplasms by Histologic Type::Neoplasms, Glandular and Epithelial::Carcinoma::Carcinoma, Squamous Celles_ES
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Structures::Genome::Genome Components::Genes::Genes, Neoplasm::Oncogeneses_ES
dc.titleThe Roles of Imprinted SLC22A18 and SLC22A18AS Gene Overexpression Caused by Promoter CpG Island Hypomethylation as Diagnostic and Prognostic Biomarkers for Non-Small Cell Lung Cancer Patientses_ES
dc.typeresearch article
dc.type.hasVersionVoR
dspace.entity.typePublication

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