Publication:
miR-122 direct detection in human serum by time-gated fluorescence imaging.

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dc.contributor.authorGarcia-Fernandez, Emilio
dc.contributor.authorGonzalez-Garcia, M Carmen
dc.contributor.authorPernagallo, Salvatore
dc.contributor.authorRuedas-Rama, Maria J
dc.contributor.authorFara, Mario A
dc.contributor.authorLópez-Delgado, Francisco J
dc.contributor.authorDear, James W
dc.contributor.authorIlyine, Hugh
dc.contributor.authorRess, Cristina
dc.contributor.authorDíaz-Mochón, Juan J
dc.contributor.authorOrte, Angel
dc.date.accessioned2023-02-08T14:37:51Z
dc.date.available2023-02-08T14:37:51Z
dc.date.issued2019-11-27
dc.description.abstractA simple method for direct detection of microRNAs (miRs) in human serum without the use of polymerase amplification is presented, achieving low miR-122 concentrations and importantly, discerning effectively single-base sequence mutations. The method is based on the capture of target miRs with synthetic peptide nucleic acid oligomers, dynamic chemical labelling, separation with quaternary amine microplatforms and detection using time-gated fluorescence imaging.
dc.identifier.doi10.1039/c9cc08069d
dc.identifier.essn1364-548X
dc.identifier.pmid31774422
dc.identifier.unpaywallURLhttps://pubs.rsc.org/en/content/articlepdf/2019/cc/c9cc08069d
dc.identifier.urihttp://hdl.handle.net/10668/14751
dc.issue.number99
dc.journal.titleChemical communications (Cambridge, England)
dc.journal.titleabbreviationChem Commun (Camb)
dc.language.isoen
dc.organizationCentro Pfizer-Universidad de Granada-Junta de Andalucía de Genómica e Investigación Oncológica-GENYO
dc.page.number14958-14961
dc.pubmedtypeJournal Article
dc.rightsAttribution 4.0 International
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.meshFluorescent Dyes
dc.subject.meshHumans
dc.subject.meshLimit of Detection
dc.subject.meshMicroRNAs
dc.subject.meshOptical Imaging
dc.titlemiR-122 direct detection in human serum by time-gated fluorescence imaging.
dc.typeresearch article
dc.type.hasVersionVoR
dc.volume.number55
dspace.entity.typePublication

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