Publication:
Sensitization to Anisakis simplex species in the population of northern Morocco.

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Date

2012

Authors

Abattouy, N
Valero, A
Martín-Sánchez, J
Peñalver, M C
Lozano, J

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ESMON Publicidad
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Abstract

Objetivos: La finalidad de este estudio ha sido la de investigar la sensibilización frente a la larva L3 de Anisakis, de una población elegida al azar del norte de Marruecos.Métodos: Hemos analizado sueros de pacientes atendidos en laboratorios de análisis clínicos de Tánger y Tetuán, incluyendo una subpoblación de pescadores del puerto de Tánger. La edad de la población ha estado comprendida entre 6-83 años. Se han utilizado las técnicas de ImmunoCAP e Immunoblotting para determinar la IgE total e IgE específi ca y se ha aplicado el estadístico de 􀁲2 o el test de Fisher, para conocer el grado de signifi cación entre las distintas variables. Resultados: La seroprevalencia obtenida ha sido del 5.1%, detectándose el mayor nº de sueros positivos en el intervalo de edad de 31-43 años. La sensibilización no está asociada con la procedencia, sexo, ocupación y edad de los individuos estudiados. Entre los sueros positivos por InmunoCAP, se detectaron por inmunoblotting, numerosas bandas entre 7 kDa y >209 kDa, predominando las de ~20 kDa – 24 kDa. Conclusiones: Aunque la anisakiasis humana por ahora no ha sido denunciada en Marruecos, nosotros aportamos datos acerca de la sensibilización con proteínas de Anisakis, de una población elegida al azar en el norte de Marruecos
OBJECTIVE To investigate sensitization to third-stage Anisakis simplex larvae in a randomly selected population in northern Morocco. METHODS We studied sera obtained from clinical analysis laboratories in Tangier and Tetuouan and from fishermen at Tangier port. The age of the study population ranged from 6 to 83 years. ImmunoCAP and immunoblotting techniques were used to determine total and specific immunoglobulin (Ig) E values and the chi2 and Fisher exact tests were applied to analyze relationships between study variables. RESULTS A seroprevalence of 5.1% was found, with a higher percentage of positive sera in the 31-to-43-year age group. Sensitization was not significantly associated with the origin, sex, occupation, or age of the individuals studied. In sera positive by InmunoCAP, immunoblotting studies detected numerous bands of between 7 kDa and >209 kDa, with a predominance of bands in the approximately 20-kDa to 24-kDa range. CONCLUSIONS Although no cases of human anisakiasis have been reported in Morocco to date, part of a randomly selected population in Northern Morocco shows sensitization to A simplex proteins.

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Journal Article; Research Support, Non-U.S. Gov't;

MeSH Terms

Medical Subject Headings::Named Groups::Persons::Age Groups::Adult
Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Aged
Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Aged::Aged, 80 and over
Medical Subject Headings::Organisms::Eukaryota::Animals
Medical Subject Headings::Organisms::Eukaryota::Animals::Invertebrates::Helminths::Nematoda::Secernentea::Ascaridida::Ascaridoidea::Anisakis
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Immunoproteins::Immunoglobulins::Antibodies::Antibodies, Helminth
Medical Subject Headings::Named Groups::Persons::Age Groups::Child
Medical Subject Headings::Check Tags::Female
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Diseases::Immune System Diseases::Hypersensitivity
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Immunologic Techniques::Immunoassay::Immunoblotting
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Immunoproteins::Immunoglobulins::Antibodies::Immunoglobulin Isotypes::Immunoglobulin E
Medical Subject Headings::Check Tags::Male
Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Middle Aged
Medical Subject Headings::Geographicals::Geographic Locations::Africa::Africa, Northern::Morocco
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Seroepidemiologic Studies
Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Young Adult
Medical Subject Headings::Named Groups::Persons::Age Groups::Adolescent

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Keywords

Anisakis, Morocco, Sensitization, IgE, Allergens, Marruecos, Sensibilización, Inmunotransferencia, Estudios Seroepidemiológicos, Inmunoglobulina E

Citation

Abattouy N, Valero A, Martín-Sánchez J, Peñalver MC, Lozano J. Sensitization to Anisakis simplex species in the population of northern Morocco. J Investig Allergol Clin Immunol. 2012; 22(7):514-9