Publication: The influence of the carrier molecule on amoxicillin recognition by specific IgE in patients with immediate hypersensitivity reactions to betalactams.
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Identifiers
Date
2016-10-12
Authors
Ariza, Adriana
Mayorga, Cristobalina
Salas, Maria
Doña, Inmaculada
Martin-Serrano, Angela
Perez-Inestrosa, Ezequiel
Perez-Sala, Dolores
Guzman, Antonio E
Montañez, Maria I
Torres, Maria J
Advisors
Journal Title
Journal ISSN
Volume Title
Publisher
Nature Publishing Group
Abstract
The optimal recognition of penicillin determinants, including amoxicillin (AX), by specific IgE antibodies is widely believed to require covalent binding to a carrier molecule. The nature of the carrier and its contribution to the antigenic determinant is not well known. Here we aimed to evaluate the specific-IgE recognition of different AX-derived structures. We studied patients with immediate hypersensitivity reactions to AX, classified as selective or cross-reactors to penicillins. Competitive immunoassays were performed using AX itself, amoxicilloic acid, AX bound to butylamine (AXO-BA) or to human serum albumin (AXO-HSA) in the fluid phase, as inhibitors, and amoxicilloyl-poli-L-lysine (AXO-PLL) in the solid-phase. Two distinct patterns of AX recognition by IgE were found: Group A showed a higher recognition of AX itself and AX-modified components of low molecular weights, whilst Group B showed similar recognition of both unconjugated and conjugated AX. Amoxicilloic acid was poorly recognized in both groups, which reinforces the need for AX conjugation to a carrier for optimal recognition. Remarkably, IgE recognition in Group A (selective responders to AX) is influenced by the mode of binding and/or the nature of the carrier; whereas IgE in Group B (cross-responders to penicillins) recognizes AX independently of the nature of the carrier.
Description
MeSH Terms
Adult
Aged
Drug Hypersensitivity
Female
Haptens
Humans
Hypersensitivity, Immediate
Immunoglobulin E
Male
Middle Aged
Penicillins
Polylysine
Serum Albumin, Human
Urticaria
Young Adult
beta-Lactams
Aged
Drug Hypersensitivity
Female
Haptens
Humans
Hypersensitivity, Immediate
Immunoglobulin E
Male
Middle Aged
Penicillins
Polylysine
Serum Albumin, Human
Urticaria
Young Adult
beta-Lactams
DeCS Terms
Inmunoglobulina E
Penicilinas
Amoxicilina
Inmunoensayo
Hipersensibilidad inmediata
Albúmina sérica humana
Penicilinas
Amoxicilina
Inmunoensayo
Hipersensibilidad inmediata
Albúmina sérica humana
CIE Terms
Keywords
Amoxicillin, Anaphylaxis, Antibody Specificity, Butylamines, Carrier Proteins, Cross Reactions
Citation
Ariza A, Mayorga C, Salas M, Doña I, Martín-Serrano Á, Pérez-Inestrosa E, et al. The influence of the carrier molecule on amoxicillin recognition by specific IgE in patients with immediate hypersensitivity reactions to betalactams. Sci Rep. 2016 Oct 12;6:35113